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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
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Last seen: 14 years, 16 days
dont flame me, sterilization questions
    #889086 - 09/17/02 06:25 PM (14 years, 2 months ago)

OK so I read that you gotta PC the jar for 45-60 min. doesnt that sound excessive? does it take that long to kill bacteria and spores? what about ovens or just sitting the jar on a washcloth and sticking it in a pan and let it heat up for 10 min?
if it gets contamunated, isnt it due to inocculating where you have to stick the syringe in the 4 holes on the top?


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InvisibleServator
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889095 - 09/17/02 06:30 PM (14 years, 2 months ago)

not gonna flame...
NOT....GOING...TO..FLAME....
Yes it's all necessary, it takes time for the heat to fully penetrate all of the substrate. Anything less WILL contaminate. If it weren't necessary, trust me, the experts wouldn't do it.


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
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Last seen: 14 years, 16 days
Re: dont flame me, sterilization questions [Re: Servator]
    #889125 - 09/17/02 06:39 PM (14 years, 2 months ago)

good answer there. thanks for not flaming me :smile: so hows anger management class going?  :wink: :cool:

one more ?, will the oven do or must a PC be used?


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InvisibleTM
The Mind, The Many, The Music.
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889166 - 09/17/02 06:55 PM (14 years, 2 months ago)

*flick!* *flick!* *flick!* ...DAMN!! Lighter's outa butane!! :mad: 


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================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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OfflineShaw
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Registered: 06/28/01
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Last seen: 8 months, 21 days
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889187 - 09/17/02 07:04 PM (14 years, 2 months ago)

i'll tell you what i told Z-tard. You don't even have to sterilize them. Just stick your thumb up your ass. Trust me, it helps make them steril.


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InvisibleServator
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889193 - 09/17/02 07:08 PM (14 years, 2 months ago)

Eh, I'm feelin happy today  :tongue:
oven works for pasteurizing bulk substrates, pretty sure it's gotta be pc for jars. PF's got a method that can be used with a regular pot, check www.fanaticus.com if you don't have a pc'er.


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Servator]
    #889205 - 09/17/02 07:13 PM (14 years, 2 months ago)



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InvisibleServator
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Re: dont flame me, sterilization questions [Re: matts]
    #889209 - 09/17/02 07:15 PM (14 years, 2 months ago)

Yea, but I flame this kid so damn much. I feel like I owe him a freebie....
:grin:


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Servator]
    #889216 - 09/17/02 07:18 PM (14 years, 2 months ago)



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OfflineSkikid16
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Registered: 06/27/02
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889217 - 09/17/02 07:18 PM (14 years, 2 months ago)

"if it gets contamunated, isnt it due to inocculating where you have to stick the syringe in the 4 holes on the top? "

The way that you have worded this makes it very hard to see what the question is. Contamination can be, and most of the time is introduced through innoculation, either due to a contaminated syringe, or filthy working conditions. Or it can be caused by improper sterilization.

About the 45-60 min in the PC, I've read some people only PC for like 20 min. (do a search to be sure.)


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Re-Defeat Bush in '04


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Skikid16]
    #889219 - 09/17/02 07:19 PM (14 years, 2 months ago)



Edited by matts (09/17/02 07:20 PM)


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OfflineXiC_clone
Calls Shots 'Round Here
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889282 - 09/17/02 07:42 PM (14 years, 2 months ago)

not..........................going......................to..................flame......

::runs away::


-XiC


--------------------
Don't soak your WBS dummy! There's an =>easy<= way.
For Your Health!


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Offlinecroftsk
cupcake

Registered: 07/19/02
Posts: 278
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889522 - 09/17/02 09:34 PM (14 years, 2 months ago)

ya know, USAlaser would have been a much cooler name! :tongue: 


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Expose yourself to your deepest fear; after that, fear has no power, and the fear of freedom shrinks and vanishes.
You are free. - Jim Morrison


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OfflineShaw
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Re: dont flame me, sterilization questions [Re: XiC_clone]
    #889524 - 09/17/02 09:34 PM (14 years, 2 months ago)

yes you are you fucking liar


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InvisibleTM
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Re: dont flame me, sterilization questions [Re: croftsk]
    #889539 - 09/17/02 09:39 PM (14 years, 2 months ago)

I think he should've chosen "IMALoser" LOL! :grin: 


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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OfflineXiC_clone
Calls Shots 'Round Here
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889560 - 09/17/02 09:47 PM (14 years, 2 months ago)

If it is just the time that bothers ya, 20 mins @ 15 PSI will do the job for :
PF tek cakes
Wheat Berries
PF wheat berry cakes
Inverto's

I caught my first jar of contam about 3 weeks ago. I am guessing it was the shorter PC'ing time. But waiting for it to cool after 20 min's is a big difference than an hour. So I like the 20 min style.

-XiC


--------------------
Don't soak your WBS dummy! There's an =>easy<= way.
For Your Health!


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 14 years, 16 days
Re: dont flame me, sterilization questions [Re: TM]
    #889569 - 09/17/02 09:52 PM (14 years, 2 months ago)

:laugh: heh! good idea but too late for that. maybe USEDbyLSA? how about LSAbuser?
or perhaps LSA2LSD

ok seriously do people ever break jars to get the mycellum cake out? someone said to break em. wont you get tiny pieces of glass, damage the cake and make a mess? also can you break the cake into pieces getting it out of the jar and bury it under soil in the fruiting container and let the shrooms grow out from the soil?
about the 4 holes, can 3 or even just 2 holes be used to reduce contamination?


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OfflineShaw
hick

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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889577 - 09/17/02 09:57 PM (14 years, 2 months ago)

do you like getting flamed? whoever said that is an idiot.


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InvisibleServator
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Re: dont flame me, sterilization questions [Re: LSAuser]
    #889581 - 09/17/02 09:58 PM (14 years, 2 months ago)

Ya gotta do what you gotta do...
Leave it at 4 for air exchange, makes it easier to innoculate more evenly as well. I don't think there would be any advantage to fewer holes.


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 14 years, 16 days
Re: dont flame me, sterilization questions [Re: Servator]
    #889594 - 09/17/02 10:04 PM (14 years, 2 months ago)

if air exchange was desired why block them using aluminum foil?


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