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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 21 years, 5 months
dont flame me, sterilization questions
    #889086 - 09/17/02 04:25 PM (21 years, 7 months ago)

OK so I read that you gotta PC the jar for 45-60 min. doesnt that sound excessive? does it take that long to kill bacteria and spores? what about ovens or just sitting the jar on a washcloth and sticking it in a pan and let it heat up for 10 min?
if it gets contamunated, isnt it due to inocculating where you have to stick the syringe in the 4 holes on the top?

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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889095 - 09/17/02 04:30 PM (21 years, 7 months ago)

not gonna flame...
NOT....GOING...TO..FLAME....
Yes it's all necessary, it takes time for the heat to fully penetrate all of the substrate. Anything less WILL contaminate. If it weren't necessary, trust me, the experts wouldn't do it.

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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 21 years, 5 months
Re: dont flame me, sterilization questions [Re: Servator]
    #889125 - 09/17/02 04:39 PM (21 years, 7 months ago)

good answer there. thanks for not flaming me :smile: so hows anger management class going?  :wink: :cool:

one more ?, will the oven do or must a PC be used?

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InvisibleTM
The Mind, The Many, The Music.
Male User Gallery

Folding@home Statistics
Registered: 06/11/02
Posts: 8,282
Loc: Under The Table And Dream...
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889166 - 09/17/02 04:55 PM (21 years, 7 months ago)

*flick!* *flick!* *flick!* ...DAMN!! Lighter's outa butane!! :mad: 


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================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:

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InvisibleShaw

Registered: 06/27/01
Posts: 2,263
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889187 - 09/17/02 05:04 PM (21 years, 7 months ago)

i'll tell you what i told Z-tard. You don't even have to sterilize them. Just stick your thumb up your ass. Trust me, it helps make them steril.


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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889193 - 09/17/02 05:08 PM (21 years, 7 months ago)

Eh, I'm feelin happy today  :tongue:
oven works for pasteurizing bulk substrates, pretty sure it's gotta be pc for jars. PF's got a method that can be used with a regular pot, check www.fanaticus.com if you don't have a pc'er.

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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Servator]
    #889205 - 09/17/02 05:13 PM (21 years, 7 months ago)


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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: matts]
    #889209 - 09/17/02 05:15 PM (21 years, 7 months ago)

Yea, but I flame this kid so damn much. I feel like I owe him a freebie....
:grin:

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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Servator]
    #889216 - 09/17/02 05:18 PM (21 years, 7 months ago)


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 19 years, 23 days
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889217 - 09/17/02 05:18 PM (21 years, 7 months ago)

"if it gets contamunated, isnt it due to inocculating where you have to stick the syringe in the 4 holes on the top? "

The way that you have worded this makes it very hard to see what the question is. Contamination can be, and most of the time is introduced through innoculation, either due to a contaminated syringe, or filthy working conditions. Or it can be caused by improper sterilization.

About the 45-60 min in the PC, I've read some people only PC for like 20 min. (do a search to be sure.)


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Re-Defeat Bush in '04

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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: Skikid16]
    #889219 - 09/17/02 05:19 PM (21 years, 7 months ago)


Edited by matts (09/17/02 05:20 PM)

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OfflineXiC_clone
Calls Shots 'Round Here
 User Gallery

Registered: 08/27/02
Posts: 505
Loc: Earth 25XX
Last seen: 21 days, 20 hours
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889282 - 09/17/02 05:42 PM (21 years, 7 months ago)

not..........................going......................to..................flame......

::runs away::


-XiC


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Don't soak your WBS dummy! There's an =>easy<= way.
For Your Health!

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Offlinecroftsk
cupcake

Registered: 07/19/02
Posts: 278
Last seen: 19 years, 1 month
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889522 - 09/17/02 07:34 PM (21 years, 7 months ago)

ya know, USAlaser would have been a much cooler name! :tongue: 


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Expose yourself to your deepest fear; after that, fear has no power, and the fear of freedom shrinks and vanishes.
You are free. - Jim Morrison

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InvisibleShaw

Registered: 06/27/01
Posts: 2,263
Re: dont flame me, sterilization questions [Re: XiC_clone]
    #889524 - 09/17/02 07:34 PM (21 years, 7 months ago)

yes you are you fucking liar


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InvisibleTM
The Mind, The Many, The Music.
Male User Gallery

Folding@home Statistics
Registered: 06/11/02
Posts: 8,282
Loc: Under The Table And Dream...
Re: dont flame me, sterilization questions [Re: croftsk]
    #889539 - 09/17/02 07:39 PM (21 years, 7 months ago)

I think he should've chosen "IMALoser" LOL! :grin: 


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:

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OfflineXiC_clone
Calls Shots 'Round Here
 User Gallery

Registered: 08/27/02
Posts: 505
Loc: Earth 25XX
Last seen: 21 days, 20 hours
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889560 - 09/17/02 07:47 PM (21 years, 7 months ago)

If it is just the time that bothers ya, 20 mins @ 15 PSI will do the job for :
PF tek cakes
Wheat Berries
PF wheat berry cakes
Inverto's

I caught my first jar of contam about 3 weeks ago. I am guessing it was the shorter PC'ing time. But waiting for it to cool after 20 min's is a big difference than an hour. So I like the 20 min style.

-XiC


--------------------
Don't soak your WBS dummy! There's an =>easy<= way.
For Your Health!

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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 21 years, 5 months
Re: dont flame me, sterilization questions [Re: TM]
    #889569 - 09/17/02 07:52 PM (21 years, 7 months ago)

:laugh: heh! good idea but too late for that. maybe USEDbyLSA? how about LSAbuser?
or perhaps LSA2LSD

ok seriously do people ever break jars to get the mycellum cake out? someone said to break em. wont you get tiny pieces of glass, damage the cake and make a mess? also can you break the cake into pieces getting it out of the jar and bury it under soil in the fruiting container and let the shrooms grow out from the soil?
about the 4 holes, can 3 or even just 2 holes be used to reduce contamination?

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InvisibleShaw

Registered: 06/27/01
Posts: 2,263
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889577 - 09/17/02 07:57 PM (21 years, 7 months ago)

do you like getting flamed? whoever said that is an idiot.


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InvisibleServator
Viral Agent

Registered: 08/30/02
Posts: 4,893
Re: dont flame me, sterilization questions [Re: LSAuser]
    #889581 - 09/17/02 07:58 PM (21 years, 7 months ago)

Ya gotta do what you gotta do...
Leave it at 4 for air exchange, makes it easier to innoculate more evenly as well. I don't think there would be any advantage to fewer holes.

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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 21 years, 5 months
Re: dont flame me, sterilization questions [Re: Servator]
    #889594 - 09/17/02 08:04 PM (21 years, 7 months ago)

if air exchange was desired why block them using aluminum foil?

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