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OfflineParesthesia
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Registered: 07/02/08
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Pioppino mycelium or contamination? (Now with OAT BRAN, and Pics)
    #8870851 - 09/03/08 10:18 AM (15 years, 5 months ago)

Pics will follow soon, I promise!

A little over a week ago, as an experiment, I made up some jars using those ziploc twist-n-seal things.  They were filled with a 30/70 mixture of  leftover rye grain from my last jar run and finely shredded hardwood mulch.  I used vermiculite as a filter layer.  These were PCd for a little over an hour and allowed to cool overnight.  (My PC lid is sticking a lot!  Argh.)

I just obtained a black poplar mushroom liquid culture syringe, and used part of it to inoculate the jars.  I didn't have much to spare so I only used about 1.5 ccs per jar and used the rest to make a liquid culture.

Well, the syringe had lumps in it.  A chunk at the tip and a huge one in the back.  When I inoculated my first jar I had to force it a bit to dislodge the chunk blocking the opening.

Since I used such a small amount of LC I wasn't expecting much.  Five of the jars are colonizing veeeery slowly, and the mycelium is almost impossible to see at this point.  However, the first jar saw an immediate explosion of growth.  Within five days it was over 50% colonized, and of course this makes me worry.

The plastic isn't completely clear, but from what I can see the mycelium is very fluffy and there is only one discolored spot near the injection site.  It's greenish yellow.

Of course I isolated the jar immediately!

So what could it be?  Is it that solid nugget of mycelium from the syringe, or a contaminant?  (It was a fresh needle, flame sterilized and injected under an alcohol soaked paper towel.)  I will post pics when I get home from work.



Edited by Paresthesia (09/04/08 08:52 PM)


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Offlineb3jamboree
yes we have no portabellas
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Re: Pioppino mycelium or contamination? [Re: Paresthesia]
    #8872358 - 09/03/08 03:41 PM (15 years, 5 months ago)

In my experience pioppino myc is pretty slow, around the same rate as shiitake. I would defiantly wonder if I had a jar colonize that quickly.


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OfflineParesthesia
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Registered: 07/02/08
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Re: Pioppino mycelium or contamination? [Re: b3jamboree]
    #8872617 - 09/03/08 04:39 PM (15 years, 5 months ago)

Awesome.  I may have to get the vendor to send me a new culture.

Is there a faster way than making up BRF cakes to tell if my liquid culture is contaminated?  I don't know if I'll be able to tell much from a dribble of LC on agar, especially peroxided agar.


--------------------
"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."

- T. S. Eliot


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OfflineSuchSmartMonkeys
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Re: Pioppino mycelium or contamination? [Re: Paresthesia]
    #8874117 - 09/03/08 09:39 PM (15 years, 5 months ago)

all you need is a drop of LC onto agar for it to work (if you've got a good colonized LC...).  If the LC is contaminated, it will grow contaminates on your petri plates...


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OfflineParesthesia
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Re: Pioppino mycelium or contamination? [Re: SuchSmartMonkeys]
    #8879412 - 09/04/08 08:52 PM (15 years, 5 months ago)





Here it is, fully colonized!  Or... fully contaminated.  That little brownish yellow spot is the inoculation point where the syringe touched the plastic.  The rest is white and fluffy looking.  It's been about a week and a half, I think.  Two?  I really need to start labeling jars and putting dates on them.


--------------------
"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."

- T. S. Eliot


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Offlineb3jamboree
yes we have no portabellas
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Re: Pioppino mycelium or contamination? [Re: Paresthesia]
    #8879488 - 09/04/08 09:10 PM (15 years, 5 months ago)

Doesn't look like any Pioppino I've ever seen.


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OfflineParesthesia
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Re: Pioppino mycelium or contamination? [Re: b3jamboree]
    #8879553 - 09/04/08 09:31 PM (15 years, 5 months ago)

Alright, how about this?!



Is that cobweb mold?  The horror... the horror...


--------------------
"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."

- T. S. Eliot


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OfflineFreeSporePrints
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Re: Pioppino mycelium or contamination? [Re: Paresthesia]
    #8886974 - 09/06/08 01:46 PM (15 years, 5 months ago)

It seems a kind of mold of the genus Trichoderma.

I'm sorry but you've to discharge the jars. Very strange however the white mycelium of the mold, without any spore.

You'll be luck the next time!
Fabio


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Offlinespazn420
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Re: Pioppino mycelium or contamination? [Re: FreeSporePrints]
    #8888114 - 09/06/08 07:18 PM (15 years, 5 months ago)

Thats pin mold - Rhizopus spp. just trash it.


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OfflineFreeSporePrints
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Re: Pioppino mycelium or contamination? [Re: spazn420]
    #8889825 - 09/07/08 06:46 AM (15 years, 5 months ago)

yes! Rhizopus:


Fabio


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OfflineParesthesia
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Re: Pioppino mycelium or contamination? [Re: FreeSporePrints]
    #8890536 - 09/07/08 11:34 AM (15 years, 5 months ago)

Wait, pin mold?  This isn't making spores, it's just gray... fluff.

The other pioppino jars I inoculated are growing very slowly, and the mycelium is fine and almost silvery. 

I'm going to take that jar out into the garage and introduce it to "fruiting conditions."  In a closed container, of course.  Bwahaha.


--------------------
"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."

- T. S. Eliot


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