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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Vermiculite

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Offlinesusej
we like cheese.
Registered: 05/20/02
Posts: 59
Last seen: 14 years, 2 months
sterile environment a waste of time?
    #888983 - 09/17/02 05:40 PM (14 years, 2 months ago)

This post concerns PF-tek and the preparations before you through your jars into the pressure cooker...I am posting after reading this post by FD3000 where he/she stated:

"It doesnt matter if you washed your hands, you can piss in a jar(maybe not) and as long as you pressure cook it there's no problem!"

The original post here

So is it a waste of time to methodically sterilize our work-area prior to pressure cooking if the PCin' is going to kill all the bacteria anyway? This would be an incredible time-saver if this is true!


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OfflineLSAuser
Full figuredwomen rule!
Registered: 08/24/02
Posts: 1,369
Loc: Florida
Last seen: 14 years, 19 days
Re: sterile environment a waste of time? [Re: susej]
    #889002 - 09/17/02 05:46 PM (14 years, 2 months ago)

I think if you PC it or even just pour boiling water all bacteria will die


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OfflineI_Fart_Blue
Stranger

Registered: 06/25/02
Posts: 3,495
Loc: SItting on the Group W Be...
Last seen: 12 years, 10 months
Re: sterile environment a waste of time? [Re: susej]
    #889008 - 09/17/02 05:48 PM (14 years, 2 months ago)

Nope it is not a waste of time. We sterilize the work area to reduce the risk of bacteria and baddies in the air and on work surfaces. PC'ing is good. It is great. It is not foolproof. Because it is not 100% effective it is essential to try and reduce the number of contams that your material is exposed to. This is a science which does take some patience. It shouldn't take that long to sterilize your work area, depending on the size. With out it, you may save 5-10 minutes in a process that takes a few weeks from start to beginning. Instead of saving 10 minutes you could wind up loosing weeks worth of work. Why risk it?


--------------------
"A study of the history of opinion is a necessary preliminary to the emancipation of the mind. I do not know which makes a man more conservative-to know nothing but the present, or nothing but the past." -John Maynard Keynes


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OfflineI_Fart_Blue
Stranger

Registered: 06/25/02
Posts: 3,495
Loc: SItting on the Group W Be...
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Re: sterile environment a waste of time? [Re: LSAuser]
    #889011 - 09/17/02 05:49 PM (14 years, 2 months ago)

Don't listen to LSAuser either. He knows not of which he speaks.


--------------------
"A study of the history of opinion is a necessary preliminary to the emancipation of the mind. I do not know which makes a man more conservative-to know nothing but the present, or nothing but the past." -John Maynard Keynes


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: sterile environment a waste of time? [Re: susej]
    #889016 - 09/17/02 05:52 PM (14 years, 2 months ago)

Pre PC needs not be sterile. After PC, be as clean as possible. Simple as that.

6T :tongue:


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineMortMtroN
journeyman
Registered: 09/09/02
Posts: 62
Last seen: 14 years, 1 month
Re: sterile environment a waste of time? [Re: SixTango]
    #889072 - 09/17/02 06:18 PM (14 years, 2 months ago)

You should keep the area you work in pretty clean anyway.


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Offlinezazz
mini F+

Registered: 05/28/02
Posts: 294
Last seen: 11 years, 9 months
Re: sterile environment a waste of time? [Re: LSAuser]
    #889117 - 09/17/02 06:37 PM (14 years, 2 months ago)

i dont go through all the trouble most people do, i just mix in a clean pot or bowl, with a clean spoon. throw in some tap water, sometiems i wipe the rim of the jar before i add the dry verm layer. then cook in a pot with water for an hour and that does fine. i havent experienced any visible contams yet, only some jars dont grow (that happened once) and some jars stop growing and/or look darker and some smell bad.

most has to do with steralizing the needle and making sure no contams get in the jars after or during innoculation


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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: sterile environment a waste of time? [Re: SixTango]
    #889175 - 09/17/02 06:58 PM (14 years, 2 months ago)

In reply to:

Pre PC needs not be sterile. After PC, be as clean as possible. Simple as that.



I agree completely. I handle all substrates with impunity exposing them to anything and everything before I PC them. Haven't had any issues yet.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: mickey_rourke]
    #889211 - 09/17/02 07:16 PM (14 years, 2 months ago)



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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Vermiculite

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