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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Invisibleimnotthere
Registered: 02/23/08
Posts: 343
using colonized substrate to inoculate new rye jars?
    #8882400 - 09/05/08 01:28 PM (15 years, 7 months ago)

im familiar with the whole g2g thang, and not doing too many generations.  I took some second generation jars of colonized rye (second gen meaning I had g2g from original jars to get these jars), and I spawned to coir.

all is going well in my 12 qt boxes, almost fully colonized.  I am out of spores, but would like to start additional rye jars.

Has anyone ever tried to take a small piece of the mycelium from a bulk tray to inoculate jars of rye?  If some coir got into the jars would it mess up?

Also would these jars that were g2g from the coir bulk be viable to grow?  What about if I were to g2g these to some more but of course stop there...

it would be original rye jars -> g2g to new rye jars ->
spawned to coir bulk trays -> g2g piece of mycelium to new rye jars -> then maybe to one last set of new rye jars,....

hope I didnt lose everyone there... would that final set be too many generations? 

is the original jar of rye from spores considered generation 1?  Generation may not even be the right term, i dunno...

thanks

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Offlineray40cal
omnitrippint
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Registered: 05/05/08
Posts: 1,308
Loc: midwest side
Last seen: 15 years, 4 months
Re: using colonized substrate to inoculate new rye jars? [Re: imnotthere]
    #8882452 - 09/05/08 01:36 PM (15 years, 7 months ago)

Generation is a cloning term as far as I understand. G2G isn't cloning, you still get multiple substrains put into the new jar.  Dunno about transfering colonized coir to grain, or how that would even mix well with it, i've used pieces of pf cake though...


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OfflinePan_
Pan


Registered: 09/04/08
Posts: 49
Loc: right here
Last seen: 15 years, 7 months
Re: using colonized substrate to inoculate new rye jars? [Re: ray40cal]
    #8882603 - 09/05/08 02:07 PM (15 years, 7 months ago)

"it would be original rye jars -> g2g to new rye jars ->spawned to coir bulk trays"

I would stop here or do one more g2g transfer before you spawn to bulk. If you do this you should have plenty to work with. No need to get too carried away and screw up or cause your mycelium to sinesce. You should get plenty of new spores to work with after you fruit what you have or you can put all that extra energy into isolating a good strain and keeping it viable...good luck
Pan


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"Whatever you do is evil for somebody"  -Joseph Campbell

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Invisiblegeorge castanzaM
Lord Of The Idiots!
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Registered: 10/21/02
Posts: 8,759
Trusted Cultivator
Re: using colonized substrate to inoculate new rye jars? [Re: imnotthere]
    #8882627 - 09/05/08 02:14 PM (15 years, 7 months ago)

You could also wait until you have some fruits growing and then use the actual fruit to spawn new substrate. 
Search around for the tek, I know it is here.


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KRAMER CAKES



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