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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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OfflineQuitDrinking
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Registered: 09/04/02
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Do I need H2O2 in Honey (dextrose actually) liquid
    #885568 - 09/16/02 11:47 AM (18 years, 7 months ago)

I started it 3 days ago and will use it in a few days, so do i need to add peroxide as suggested in the tek (some of them suggest it, some don't). Am I far more likely to have Contam's if i don't add it? Also do i have to chop up the threads that form or is it like a spore syringe where you don't have to see the stuff in every squirt for it to work.
My recipe was 1 tsp. of dextrose and 1/2 pint of water sterilized, cooled and added 2 cc's of PF race spore syringe...


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Invisiblemickey_rourke
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Registered: 07/18/02
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Loc: Playa del Carmen
Re: Do I need H2O2 in Honey (dextrose actually) liquid [Re: QuitDrinking]
    #885574 - 09/16/02 11:49 AM (18 years, 7 months ago)

I don't use H202 at all. When I'm ready to use the solution for innoculation, I stick the needle into the jar, suck in and squirt out the liquid several times to distribute the growth, and then fill the syringe for immediate use.


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OfflineErbaliza
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Registered: 08/31/02
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Re: Do I need H2O2 in Honey (dextrose actually) liquid [Re: mickey_rourke]
    #885591 - 09/16/02 11:57 AM (18 years, 7 months ago)

I use the 'shake the shit out of it' tek :grin:
Have never used H202 and been contam free so far(fingers crossed)...Peace 


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OnlineDERRAYLD
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Re: Do I need H2O2 in Honey (dextrose actually) liquid [Re: Erbaliza]
    #886699 - 09/16/02 09:14 PM (18 years, 7 months ago)

If you are innoculating the jar with spores then h2o2 will kill them.
If however you are cloning a mushron then the myceliul network is allready established so h2o2 will help prevent contamination.


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Offlinetchyted
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Registered: 09/03/01
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Loc: WA near seattle
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Re: Do I need H2O2 in Honey (dextrose actually) liquid [Re: QuitDrinking]
    #886734 - 09/16/02 09:39 PM (18 years, 7 months ago)

if you use hydrogen peroxide in honey water, it will cause the mycelium to float on the top of the licuid. as the enzymes that break down the H2O2 release oxeygen, it forms bubbles that make it float. this wouldn't be bad except that the top of the licuid ends up being depleated of peroxide, thus defeating the purpose of adding it in the first place.

worse yet, any mold spores that land on the surface, will have a substrate to grow on that keeps them on the surface of the licuid where mold does best, and will eventually be able to sporulate.

in short, adding peroxide, in my experiance, is actually counter-productive in licuid inoculants, with the possible exception of shortly before use.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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