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OfflineSkatterman420
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Contam Problem
    #8832821 - 08/26/08 09:09 PM (15 years, 7 months ago)

So Ive Had 10 Pre-Sterilized Mycobags
and 4 PF jars I made go bad on me

Now ive narrowed it down to
the syringes were contamd (but I doubt it)
Ive been innoculating them in my bathroom after dousing it with Oust, Im guessing this wasnt clean enough, I whiped down injection site and needle inbetween injections.

as for the PF jars I made, I used a dry verm layer and pcd 2 of them twice because my PC has no Weight for the top, I so guess I steamed them twice.

BTW: Contam = Trich

Any advice, I think Nexttime Ill just tape a bag to my arm like ive seen done.

Also i'm guessing filter patches do nothing to stop contams from getting in? As in one layer of tyvek.

Edited by Skatterman420 (08/26/08 09:11 PM)

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InvisibleMushHunter08
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Registered: 06/08/08
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Re: Contam Problem [Re: Skatterman420]
    #8833052 - 08/26/08 09:45 PM (15 years, 7 months ago)

You are inoculating with a glove box or in just an open air environment?


--------------------
"The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough."
-William Blake-

The most simple method of growing mushrooms:
www.mushroomvideos.com

MultiSync's lazy bastard print/syringe guide

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OfflineSkatterman420
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Re: Contam Problem [Re: MushHunter08]
    #8833103 - 08/26/08 09:53 PM (15 years, 7 months ago)

open air using the small room method(i see the flaws in this one now), with gloves and facemask whiping syringe with alcohol

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OfflineSkatterman420
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Re: Contam Problem [Re: Skatterman420]
    #8833109 - 08/26/08 09:54 PM (15 years, 7 months ago)

oh and I dug them all holes and covered with verm and a coir/dpoo mix

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InvisibleMushHunter08
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Re: Contam Problem [Re: Skatterman420]
    #8833128 - 08/26/08 09:58 PM (15 years, 7 months ago)

Get yourself a glove box brother. It should help a lot. Very easy to make and not expensive at all, and they will save you so many headaches. Good luck in the future.


--------------------
"The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough."
-William Blake-

The most simple method of growing mushrooms:
www.mushroomvideos.com

MultiSync's lazy bastard print/syringe guide

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InvisibleLoveOverAll
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Re: Contam Problem [Re: MushHunter08]
    #8833369 - 08/26/08 10:45 PM (15 years, 7 months ago)

i have a small bathroom so after i shower and the room is soaked i spray it down with water/bleach, after 10 mins i set up and innoc, ive done this 4x now and havent seen contaims yet:syringe:

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OfflineSkatterman420
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Re: Contam Problem [Re: LoveOverAll]
    #8834656 - 08/27/08 07:53 AM (15 years, 7 months ago)

Quote:

theblackhole said:
i have a small bathroom so after i shower and the room is soaked i spray it down with water/bleach, after 10 mins i set up and innoc, ive done this 4x now and havent seen contaims yet:syringe:





Wow how come I never thought of this, the moisture in the air would keep the mold spores weighed down i'd think.

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Offlineveda_sticks
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Re: Contam Problem [Re: Skatterman420]
    #8834718 - 08/27/08 08:09 AM (15 years, 7 months ago)

Bathroom is one of the worst places you could use, great breading ground for black mould.

Build a simple glove box. You dont even have to oust/lysol/bleach bomb it. Just wipe the walls down with some bleach solution.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineUncleMike
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Re: Contam Problem [Re: Skatterman420]
    #8834729 - 08/27/08 08:12 AM (15 years, 7 months ago)

Quote:

Skatterman420 said:
Quote:




Wow how come I never thought of this, the moisture in the air would keep the mold spores weighed down i'd think.




or send them into a super freenzy feeding stage and they begin to devour you:abduction:


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Live each day like it will be your last, tomorrow my never come.
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OfflineSkatterman420
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Re: Contam Problem [Re: veda_sticks]
    #8842730 - 08/28/08 04:27 PM (15 years, 6 months ago)

i'd imagine this is my best bet, I have some toilet flanges and long sleeved gloves. Just gotta throw it together I suppose.

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OfflineSkatterman420
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Re: Contam Problem [Re: UncleMike]
    #8842774 - 08/28/08 04:36 PM (15 years, 6 months ago)

Quote:

UncleMike said:
or send them into a super freenzy feeding stage and they begin to devour you:abduction:




Oh god, don't say that I start feeling goofy after I think I've breathed in some Moldspores. Ever since I started reading up on mycology i've thought alot harder and deeper about what lands on my food and drink and surfaces that are gross its like I can feel the mold spores :/

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Offlinejvm
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Re: Contam Problem [Re: Skatterman420]
    #8842870 - 08/28/08 04:54 PM (15 years, 6 months ago)

I used a small bathroom too after sterilizing it the best i could by cleaning the whole bathroom and removing ALL the items in there. Disinfected it a few times over a few hours and before i went in there. I also used a easy to make glove box. Just use a cardboard box and put plastic wrap over the top with two holes for your hands on the sides. Always rub your gloves and arms down with iso alcohol before entering the glove box.

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OfflineGojira45
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Re: Contam Problem [Re: Skatterman420]
    #8842873 - 08/28/08 04:54 PM (15 years, 6 months ago)

Quote:

Skatterman420 said:
Im guessing this wasnt clean enough, I whiped down injection site and needle inbetween injections.

Also i'm guessing filter patches do nothing to stop contams from getting in? As in one layer of tyvek.




Just curious - when you say wipe down, what do you mean exactly? Do you know the wiping surface is clean? Sure you are not just spreading contams onto the innoc port by not wiping with a contam free wipe?

Also, you are not flaming your syringes? I just set up a propane torch & make the needle nice & red...

Well, filter patches are better than having nothing there - but I don't think tyvek is rated to stop passage of biological material - but with the pressure regulated on each side, it shouldn't be sucking any in either...


--------------------
"The theft of the seals and the possibility of a Planet X really point to everything we are doing as humans.

It was all set up. Were doing the same thing Hitler did with artifacts and knowledge about hollow earth.

Everthing points to hollow earth and that ultimitaly points to planet X." 


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OfflineSkatterman420
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Re: Contam Problem [Re: Gojira45]
    #8843209 - 08/28/08 06:05 PM (15 years, 6 months ago)

By whiping down I mean with alcohol, is this not enough, should I flame sterilize?

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Invisiblethemange
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Re: Contam Problem [Re: Skatterman420]
    #8843236 - 08/28/08 06:09 PM (15 years, 6 months ago)

people will say either/or. t

he truth is: nothing will live thats on your needle if you make the end red hot. nothing.

better safe than sorry! especially something so easy to do.

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OfflineSkatterman420
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Re: Contam Problem [Re: themange]
    #8844076 - 08/28/08 08:49 PM (15 years, 6 months ago)

I'll take your advice

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Offlineveda_sticks
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Re: Contam Problem [Re: Skatterman420]
    #8845836 - 08/29/08 02:41 AM (15 years, 6 months ago)

always flame your needle before each jar or bag to prevent cross contamination.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

Extras: Filter Print Post Top
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