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OfflineGreen_Velvet
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wrapping agar plates with saran wrap
    #883926 - 09/15/02 08:14 PM (19 years, 7 days ago)

Just curious if it is okay to completely wrap agar filled petri dishes with saran wrap. I have read from various sources to just wrap the edges. I found it much easier to just take a big piece of saran wrap and wrap it around the whole thing. Is that okay?


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OfflineAnnoA
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Re: wrapping agar plates with saran wrap [Re: Green_Velvet]
    #884208 - 09/15/02 09:58 PM (19 years, 7 days ago)

It doesn?t matter how you wrap it, if only by the edge or as whole. To me it?s easyer if i wrat i t only by the edge.

As for the wraping material, saran wrap is NOT the best choice, soince it?s water and oxygene IMPERMEABLE.

You should use Polyethylene foil wrap, like Glad Cling Wrap.

http://www.glad.com/plasticwrap.html



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OfflineShaw
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Re: wrapping agar plates with saran wrap [Re: Green_Velvet]
    #884213 - 09/15/02 09:59 PM (19 years, 7 days ago)

as long as the edges are sealed.


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Offlinemixpix
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Re: wrapping agar plates with saran wrap [Re: Shaw]
    #885251 - 09/16/02 09:36 AM (19 years, 7 days ago)

I thought cling wrap was the plastic garbage?


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OfflineDinoMyc
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Re: wrapping agar plates with saran wrap [Re: Green_Velvet]
    #886332 - 09/16/02 07:03 PM (19 years, 6 days ago)

Just spring for some parafilm, its worth it and specifically designed for what you are doing.
I believe they may make more than one style, gas permiable being one of those.
best of luck.


--------------------
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InvisibleRoadkillM
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Re: wrapping agar plates with saran wrap [Re: DinoMyc]
    #886851 - 09/16/02 11:31 PM (19 years, 6 days ago)

Dino,

Your gonna laugh at me.

I just use black electrical tape.

I haven't had a contam yet on agar.

:grin:

 


--------------------
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OfflineAnnoA
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Re: wrapping agar plates with saran wrap [Re: Roadkill]
    #886917 - 09/17/02 12:33 AM (19 years, 6 days ago)

HAHAHAHA @roadkill :wink:

I tried parafilm, and found the glad cling wrap do a better, faster and much, much less expensive job. 


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OfflineUnity333
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Re: wrapping agar plates with saran wrap [Re: Green_Velvet]
    #887249 - 09/17/02 05:09 AM (19 years, 6 days ago)

I sometimes use ziplock bagies to seal my plates. It works great, and green and yellow make blue...


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Offlinejonnyshaggs420
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Re: wrapping agar plates with saran wrap [Re: Roadkill]
    #887759 - 09/17/02 10:01 AM (19 years, 6 days ago)

I too use the black electrical tape for my dishes.......


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Offlinepleezr
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Re: wrapping agar plates with saran wrap [Re: jonnyshaggs420]
    #889897 - 09/18/02 02:08 AM (19 years, 5 days ago)

As do I....er...did I.


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InvisibleRoadkillM
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Re: wrapping agar plates with saran wrap [Re: pleezr]
    #891310 - 09/18/02 04:20 PM (19 years, 4 days ago)

I'm switching to red electrical tape!

I wanna be different.  :wink:

 


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineShaw
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Re: wrapping agar plates with saran wrap [Re: Roadkill]
    #892054 - 09/18/02 09:59 PM (19 years, 4 days ago)

I've used electrical tape too. works just fine, and creates a perfect seal.


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Offlinemattymonkey
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Re: wrapping agar plates with saran wrap [Re: Roadkill]
    #4435675 - 07/21/05 11:18 PM (16 years, 2 months ago)

Hey RoadKill... THANKS!! ran outa parafilm tonight.. 1/4 of the way done... quick search on the shroomery, pulled this up, and im saved!

many blessings bro
peace

and give thanks for the shroomery! :smile:


--------------------
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OfflineRogerRabbitM
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Re: wrapping agar plates with saran wrap [Re: mattymonkey]
    #4447572 - 07/24/05 07:22 PM (16 years, 1 month ago)

Electrical tape is waterproof, so eventually it seems the dishes will run out of oxygen. Have any of you noticed the growth stalling after awhile?

A tip for cutting the price of parafilm in half is to cut it lengthwise before wrapping, so each piece can wrap two dishes. I wrap 1,000 petri dishes that way with each $20 roll of parafilm, which works out to only 2 cents per dish.
RR


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Invisiblethemills
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Re: wrapping agar plates with saran wrap [Re: RogerRabbit]
    #4448898 - 07/25/05 01:05 AM (16 years, 1 month ago)

Now thats what I call thinking! I can live with 2 cents a plate


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Offlinepshawny
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Re: wrapping agar plates with saran wrap [Re: Unity333]
    #4452993 - 07/26/05 12:36 AM (16 years, 1 month ago)

Quote:

Unity333 said:
I sometimes use ziplock bagies to seal my plates. It works great, and green and yellow make blue... 




How can two colors make one, thats absurd.  :rofl: j/k 
Good idea bye the way.


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Invisibledeanofmean
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Re: wrapping agar plates with saran wrap [Re: pshawny]
    #4453770 - 07/26/05 03:04 AM (16 years, 1 month ago)

Glade wrap works grate. it is gas permeable.
if you sharpen up your carving knife, you can slice the roll up to make 6, 2" rolls.
that should last a while. :smile:

edit: i got this tip from Anno.


Edited by deanofmean (08/06/05 02:23 AM)


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Offlinemattymonkey
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Re: wrapping agar plates with saran wrap [Re: mattymonkey]
    #4455375 - 07/26/05 02:11 PM (16 years, 1 month ago)

btw guys i dont suggest electrical tape anymore... seems a few of my plates just came right off! they were never moved or anything just left alone for a few days, i get ack and a bunch are uncovered, super strange! well anyway back to the drawing board..


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OfflineNo_Life_G33k
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Re: wrapping agar plates with saran wrap [Re: mattymonkey]
    #4465682 - 07/28/05 08:13 PM (16 years, 1 month ago)

Electrical tapes seals real well, but it's a pain to remove 'in action'.... is the parafilm easy to remove when you're doing the innocualtions???


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Offlinemattymonkey
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Re: wrapping agar plates with saran wrap [Re: No_Life_G33k]
    #4476589 - 07/31/05 09:32 AM (16 years, 1 month ago)

yes its very nice and easy to work with, i fold it over and make it stronger and that makes it easier to remove as well


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Offlinehyphae
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Re: wrapping agar plates with saran wrap [Re: deanofmean]
    #4488710 - 08/03/05 07:52 AM (16 years, 1 month ago)

Quote:

deanofmean said:
Glade wrap works grate. it is gas permeable.
if you sharpen up your carving knife, you can slice the roll up to make 6, 2" rolls.
that should last a while. :smile:



I cut mine in 1" increments. Cling Wrap is the best choice IME, plates will breath and thats why parafilm was created in the first place :wink: I've also used ziplocks to protect and hold in moisture with great results and for me is the easiest.


--------------------
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Invisiblemycofile
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Re: wrapping agar plates with saran wrap [Re: hyphae]
    #4497469 - 08/05/05 12:57 AM (16 years, 1 month ago)

Does anybody know if pressnseal breathes like glad wrap? Not necessarily for agar plates, just curoius and ya'll seem to know your plastics.


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Invisibledeanofmean
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Re: wrapping agar plates with saran wrap [Re: hyphae]
    #4513516 - 08/09/05 07:10 AM (16 years, 1 month ago)

Don't get mad, get Glad  :thumbup:
sorry i just couldn't resist. :grin:


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OfflineJustlookin0
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Re: wrapping agar plates with saran wrap [Re: Green_Velvet]
    #4525038 - 08/12/05 02:50 AM (16 years, 1 month ago)

Heres an idea......Get that new glad press and seal saran wrap and cut some square peices.....I havent tried it but from the commercials it looks like a good product.


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Offlinehyphae
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Re: wrapping agar plates with saran wrap [Re: Justlookin0]
    #4525680 - 08/12/05 08:02 AM (16 years, 1 month ago)

Old idea bro, look back two posts.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineFluxburn
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Re: wrapping agar plates with saran wrap [Re: hyphae]
    #4620160 - 09/04/05 08:27 PM (16 years, 17 days ago)

Ok so if the plate wrap is gas permiable, aren't u supposed to leave the plates in a sterile environment like a flow hood or clean cab?


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OfflineFluxburn
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Re: wrapping agar plates with saran wrap [Re: hyphae]
    #4620161 - 09/04/05 08:27 PM (16 years, 17 days ago)

Ok so if the plate wrap is gas permiable, aren't u supposed to leave the plates in a sterile environment like a flow hood or clean cab?


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OfflineCrashManFlash
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Re: wrapping agar plates with saran wrap [Re: Fluxburn]
    #4621969 - 09/05/05 11:22 AM (16 years, 17 days ago)

zip lock baggies work well with the peroxide method,all work done in the old glove box of course.


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