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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker

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OfflineSamson4
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Registered: 08/21/08
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Maybe Someone has tried this
    #8813525 - 08/23/08 12:41 AM (9 years, 8 months ago)

My first crack at colonizing jars was irritating. So this time I want to make 100% sure I kill everything in my substrate. Here is my idea. I put my jars/ovenbags in a pot filled with 50/50 water and antifreeze, it would boil at 275 degrees Fahrenheit. Cover it with a lid then boil it for 45 minutes. If I wanted to raise the temperature more, Id put it in a pressure cooker at 10psi. What do you guys think of this?


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Offlinegluvbox
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Re: Maybe Someone has tried this [Re: Samson4]
    #8813718 - 08/23/08 01:29 AM (9 years, 8 months ago)

There are two problems that you will run into.

First is that the water in your substrate won't have antifreeze in it so it will still become a gas at 212°F.

The second is it that this evaporation will make your jars/ovenbag explode with pressure as their interior water vaporizes.

If your jars survive, they will be missing most of their moisture.


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Edited by gluvbox (08/23/08 01:30 AM)


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Offlineveda_sticks
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Re: Maybe Someone has tried this [Re: gluvbox]
    #8814211 - 08/23/08 04:02 AM (9 years, 8 months ago)

steam sterilise for 90 minutes. if you want to check that your substrate has been properly sterilised, leave 1 for a few weeks un inoculated

are you using a glovebox?


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Offlinepontus
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Re: Maybe Someone has tried this [Re: veda_sticks]
    #8815058 - 08/23/08 12:23 PM (9 years, 8 months ago)

I am not sure if I get your idea, but if you have a pressure cooker it makes no sense to me to previously boil your jars in antifreeze and water. Just use your PC! If it can only do 10 psi I would go for 120 minutes. What went wrong with your first batch?


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I'm locked in tight, I'm out of range
I used to care, but things have changed"
Bob Dylan


Edited by pontus (08/23/08 12:26 PM)


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OfflineCitratox
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Re: Maybe Someone has tried this [Re: pontus]
    #8815069 - 08/23/08 12:28 PM (9 years, 8 months ago)

PCing works for everyone else here, why wouldnt it you...


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Invisiblecheesenoonions
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Registered: 04/02/01
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Re: Maybe Someone has tried this [Re: Citratox]
    #8815092 - 08/23/08 12:35 PM (9 years, 8 months ago)

are these grain jars?  What substrate are you using?  IMO, if you have problems with sterilization.....PC longer.  Your PC may only reach 10 psi.  I think alot of PCs sold at stores nowadays are 10 psi.  This will require more time in the PC.

Also, if you want to figure out what the problem is.....sterilize in the PC, then let the jars sit for few days.  The problem might be your syringe or whatever you're using.


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Offlinemisos
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Re: Maybe Someone has tried this [Re: Samson4]
    #8815307 - 08/23/08 01:29 PM (9 years, 8 months ago)

PC. All the way. PC BRF for an hour at 15. Guaranteed sterile. Grains, fuck if I know, I hate them.


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"If I had a single wish, I would have every single human on this planet see this natural world the way I see it; the beauty in such simple things such as a fallen tree that is covered in moss and that has new trees growing from it. To some, fallen trees are ugly. But in reality, it is the circle of life at its finest. This is a beautiful world, its time that we recognize that before its all gone."


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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