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lipa
Registered: 07/24/07
Posts: 2,684
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Polyporus squamosa
#8808630 - 08/21/08 10:38 PM (15 years, 7 months ago) |
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Does anyone have any experience with Polyporus squamosa. I am having a hell of a time getting them to germinate. Do they require some form of stimuli to get them going?
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Polyporus squamosa [Re: lipa]
#8808877 - 08/21/08 11:46 PM (15 years, 7 months ago) |
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I've got no experience with it, but have you tried using different media with it? The addition of yeast/yeast extract seems to help with the germination of some species from what I've read.
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dstark
Manifesting Minds
Registered: 02/27/08
Posts: 4,144
Last seen: 7 months, 29 days
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Re: Polyporus squamosa [Re: wisp]
#8809080 - 08/22/08 12:30 AM (15 years, 7 months ago) |
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Yup, many gourment just wont germinate or will be very slow on (PDA), MAE is preferable.
-------------------- What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered? ~I Feelat Home~
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Polyporus squamosa [Re: dstark]
#8811543 - 08/22/08 02:25 PM (15 years, 7 months ago) |
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I have tried MYA, MEA,MYPA with no luck. I'll just keep on trying. Thanks.
Lipa
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MycoAu
5thKingdomCome
Registered: 07/18/07
Posts: 1,047
Last seen: 3 years, 3 months
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Re: Polyporus squamosa [Re: lipa]
#8811575 - 08/22/08 02:31 PM (15 years, 7 months ago) |
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Charcoal?
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: Polyporus squamosa [Re: MycoAu]
#8812829 - 08/22/08 07:55 PM (15 years, 7 months ago) |
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Thats probably a good idea for the next batch.
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Polyporus squamosa [Re: lipa]
#8813016 - 08/22/08 08:46 PM (15 years, 7 months ago) |
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How about using other organisms as a catalyst? Maybe mycelium from a slow grow species such as Hericium or even bacteria from your finger tips.
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 5 months, 19 days
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Re: Polyporus squamosa [Re: wisp]
#8814165 - 08/23/08 01:46 AM (15 years, 7 months ago) |
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Or boil some wood chips in the water you use for the agar.
Carsten
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dstark
Manifesting Minds
Registered: 02/27/08
Posts: 4,144
Last seen: 7 months, 29 days
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Re: Polyporus squamosa [Re: Mycelio]
#8814244 - 08/23/08 02:19 AM (15 years, 7 months ago) |
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I got a nice TEK thats what he wrote:
About the spores, i don't remember how much they are old but however try to make some syringes with spores in distilled water and let them in fridge for a week, then prepare common MEA but using woodchips tea instead of the common water..and let me know!
With this tecnique i've germinated some old spores of the 2002..
i asked him what he mean in wodchips team, i supose it is teanot from baggies.
-------------------- What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered? ~I Feelat Home~
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Polyporus squamosa [Re: dstark]
#8814252 - 08/23/08 02:22 AM (15 years, 7 months ago) |
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I would think woodchip tea is tea made from boiling woodchips in water, then discarding the woodchips but keeping the liquid.
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FreeSporePrints
Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy
Last seen: 3 months, 23 days
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Re: Polyporus squamosa [Re: wisp]
#8814291 - 08/23/08 03:01 AM (15 years, 7 months ago) |
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Quote:
tripsis said: I would think woodchip tea is tea made from boiling woodchips in water, then discarding the woodchips but keeping the liquid.
Yes it works exactly i this way!
I had this tip from Carsten (Mycelio) and i must say that this recipe is really a bomb!
I believe making common recipes like PDA or MEA we cannot create a FULL environment and perfect as the nature does. Boiling the woodchips we "extract" a lot of nutrients and supplements for a "natural" germination of the spores..in other words i believe the spores feels more confortable on woodchips tea agar than on MEA or PDA, like in nature.
I've tried the woodchip tea agar recipe with a sporeprint from 2002 or 2004(i cannot remember) and the spores germinated at the 2 or 3..quite neither with fresh spores we can see a germination so rapid!
I don't know if this can be a fact of relevance but i've waited a lot of time (more than 1 hour) before pouring the broth in the petri plate (thing that i never do but i should..), i had really a few of drops!
IMHO an excessive moisture content (drops of water) in the agar, blocks the spores from the germination. Is something similar to born in a pool and after die i suppose..
I've inoculated the spores soaked in distilled water a week into a syringe in fridge..
I'll take some pics!
Fabio
ps. for the bemushroomed interested in the woodchip tea agar you can find a tutorial with pics on FreeSporePrints.org (in this moment the servers are busy..check it later!)
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