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Invisiblelipa
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Polyporus squamosa
    #8808630 - 08/22/08 12:38 AM (9 years, 5 months ago)

Does anyone have any experience with Polyporus squamosa. I am having a hell of a time getting them to germinate. Do they require some form of stimuli to get them going?


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Invisiblewisp

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Re: Polyporus squamosa [Re: lipa]
    #8808877 - 08/22/08 01:46 AM (9 years, 5 months ago)

I've got no experience with it, but have you tried using different media with it? The addition of yeast/yeast extract seems to help with the germination of some species from what I've read.


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Offlinedstark
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Re: Polyporus squamosa [Re: wisp]
    #8809080 - 08/22/08 02:30 AM (9 years, 5 months ago)

Yup, many gourment just wont germinate or will be very slow on (PDA), MAE is preferable.


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Re: Polyporus squamosa [Re: dstark]
    #8811543 - 08/22/08 04:25 PM (9 years, 5 months ago)

I have tried MYA, MEA,MYPA with no luck. I'll just keep on trying. Thanks.

Lipa


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OfflineMycoAu
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Re: Polyporus squamosa [Re: lipa]
    #8811575 - 08/22/08 04:31 PM (9 years, 5 months ago)

Charcoal?


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Invisiblelipa
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Re: Polyporus squamosa [Re: MycoAu]
    #8812829 - 08/22/08 09:55 PM (9 years, 5 months ago)

Thats probably a good idea for the next batch.


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Invisiblewisp

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Re: Polyporus squamosa [Re: lipa]
    #8813016 - 08/22/08 10:46 PM (9 years, 5 months ago)

How about using other organisms as a catalyst? Maybe mycelium from a slow grow species such as Hericium or even bacteria from your finger tips.


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Re: Polyporus squamosa [Re: wisp]
    #8814165 - 08/23/08 03:46 AM (9 years, 5 months ago)

Or boil some wood chips in the water you use for the agar.


Carsten


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Offlinedstark
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Re: Polyporus squamosa [Re: Mycelio]
    #8814244 - 08/23/08 04:19 AM (9 years, 5 months ago)

I got a nice TEK thats what he wrote:

About the spores, i don't remember how much they are old but however try to make some syringes with spores in distilled water and let them in fridge for a week, then prepare common MEA but using woodchips tea instead of the common water..and let me know!

With this tecnique i've germinated some old spores of the 2002..

i asked him what he mean in wodchips team, i supose it is teanot from baggies.


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Invisiblewisp

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Re: Polyporus squamosa [Re: dstark]
    #8814252 - 08/23/08 04:22 AM (9 years, 5 months ago)

I would think woodchip tea is tea made from boiling woodchips in water, then discarding the woodchips but keeping the liquid.


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OfflineFreeSporePrints
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Re: Polyporus squamosa [Re: wisp]
    #8814291 - 08/23/08 05:01 AM (9 years, 5 months ago)

Quote:

tripsis said:
I would think woodchip tea is tea made from boiling woodchips in water, then discarding the woodchips but keeping the liquid.




Yes it works exactly i this way!

I had this tip from Carsten (Mycelio) and i must say that this recipe is really a bomb!

I believe making common recipes like PDA or MEA we cannot create a FULL environment and perfect as the nature does. Boiling the woodchips we "extract" a lot of nutrients and supplements for a "natural" germination of the spores..in other words i believe the spores feels more confortable on woodchips tea agar than on MEA or PDA, like in nature.

I've tried the woodchip tea agar recipe with a sporeprint from 2002 or 2004(i cannot remember) and the spores germinated at the 2 or 3..quite neither with fresh spores we can see a germination so rapid!

I don't know if this can be a fact of relevance but i've waited a lot of time (more than 1 hour) before pouring the broth in the petri plate (thing that i never do but i should..), i had really a few of drops!

IMHO an excessive moisture content (drops of water) in the agar, blocks  the spores from the germination. Is something similar to born in a pool and after die i suppose..

I've inoculated the spores soaked in distilled water a week into a syringe in fridge..

I'll take some pics!

Fabio

ps. for the bemushroomed interested in the woodchip tea agar you can find a tutorial with pics on FreeSporePrints.org (in this moment the servers are busy..check it later!)


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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