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Offlinechobumms
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On MS inoculation
    #8806946 - 08/21/08 07:42 PM (9 years, 8 months ago)

Do some of the experts here use MS? I figure they would all do spore print to agar and isolate. Correct me if I'm wrong, unless they are running random experiments like "Using MS inoculation, what percent of growth gets contaminated in the colonization run?", there is no need for MS right?


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My "Faster Germination For Popcorn Substrate" TEK
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Offlineskaterickets
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Re: On MS inoculation [Re: chobumms]
    #8807032 - 08/21/08 07:58 PM (9 years, 8 months ago)

Not that I'm an expert myself, but I look at grow logs a LOT, and none of them use MS unless it's to obtain a good performing strain. Not many I see use strain isolates on agar though, most of them just clone straight up.


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OfflineScavengerType
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Re: On MS inoculation [Re: skaterickets]
    #8807457 - 08/21/08 09:10 PM (9 years, 8 months ago)

many people who are noobs use MS LCs and if it ain't screwing them up they usually don't progress on to cloning quite quickly. However, in all my reading nobody has ever suggested MS is better and many have said strain isolates are the way to go, unless you clone a bad strain. So just don't keep all your eggs in one basket and keep master cultures and prints.


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Invisibleblood4blood
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Re: On MS inoculation [Re: ScavengerType]
    #8808127 - 08/21/08 11:09 PM (9 years, 8 months ago)

this grow was MS to LC.  but no isolates so you could still call it a MS grow
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8324968/an/0/page/0

i still do MS innoculations at the moment.  well just to a couple jars with a syringe and then i use them for a G2G transfer.


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OfflineScavengerType
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Re: On MS inoculation [Re: blood4blood]
    #8808181 - 08/21/08 11:19 PM (9 years, 8 months ago)

yea I do that too but I cannot step to the grows Large dose has in those pics. I mean fruiting has completely stopped vertical FAE in those tubs.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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Offlinechobumms
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Re: On MS inoculation [Re: ScavengerType]
    #8812301 - 08/22/08 07:19 PM (9 years, 8 months ago)

Thats crazy, There are alot of mushrooms in there and he didn't isolate shit!


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My "Faster Germination For Popcorn Substrate" TEK
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