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OfflineScavengerType
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lc cloning kit
    #8806677 - 08/21/08 06:51 PM (9 years, 6 months ago)

I'm looking at putting together a lc cloning kit to be ready to quickly clone monster mushes when I get some. After all processing fruited tubs is often time consuming enough and I've been doing MS for way too long.

Anywhoo I'm putting together a list of things I should include what say yee?

-Pre-pasteurized LC containers. (can they be kept uninoculated and for how long?)
-Ready syringes for tissue extraction (/w sterile water)
-my glovebox and other related equipment (like alc. and gloves).

Anything else? It seems like I'm forgetting something.


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


Edited by ScavengerType (08/21/08 07:06 PM)


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Offlineveda_sticks
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Re: lc cloning kit [Re: ScavengerType]
    #8807089 - 08/21/08 08:09 PM (9 years, 6 months ago)

you dont pasturize your lc jar, needs to be sterilised.

alcohol wipes
lighter (sterilising the needle)
possibly bleach if you gt contaim problemss.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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Offlineaaronemerritt
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Re: lc cloning kit [Re: veda_sticks]
    #8807171 - 08/21/08 08:21 PM (9 years, 6 months ago)

how many multispore projects have you done i have just finished the first flush on my multispore project have you noticed alot of potency variance in shrooms from the same flush


--------------------
Mushrooms are a funny drug!!!!!!!!!!!!!


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OfflineScavengerType
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Re: lc cloning kit [Re: aaronemerritt]
    #8807284 - 08/21/08 08:37 PM (9 years, 6 months ago)

yea I meant sterilized I don't know why I said pasteurized.

Thanks for contributing though I have most of that stuff in my glovebox. In fact I think I'll just toss this stuff in with my glovebox when I got em together.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


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Offlineveda_sticks
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Re: lc cloning kit [Re: aaronemerritt]
    #8807871 - 08/21/08 10:20 PM (9 years, 6 months ago)

Quote:

aaronemerritt said:
how many multispore projects have you done i have just finished the first flush on my multispore project have you noticed alot of potency variance in shrooms from the same flush




Potency is ginetic. Many thousands of spores used in each inoculation.

need i say more?


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineDamion5050
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Re: lc cloning kit [Re: veda_sticks]
    #8807887 - 08/21/08 10:24 PM (9 years, 6 months ago)

be careful when using your glovebox do not use a flame in it or you may melt the box to your arms and hands..


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Offlineaaronemerritt
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Re: lc cloning kit [Re: Damion5050]
    #8808793 - 08/22/08 01:26 AM (9 years, 6 months ago)

thousands of spores  but n realty shouldny the spores have the same combo of dominant and ressesive genes of there perents


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Mushrooms are a funny drug!!!!!!!!!!!!!


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InvisibleKrez
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Re: lc cloning kit [Re: aaronemerritt]
    #8808823 - 08/22/08 01:34 AM (9 years, 6 months ago)

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5874305/page/0/fpart/1/vc/1

Its all there for ya.  Good luck.  Why dont you try getting into agar?


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)


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OfflineScavengerType
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Re: lc cloning kit [Re: aaronemerritt]
    #8808828 - 08/22/08 01:34 AM (9 years, 6 months ago)

They should be... relatively similar.

But there can be a lot of variance I'm just trying to get a cloning kit together cause I want to be ready next time after missing the boat so often.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club


Post Extras: Print Post  Remind Me! Notify Moderator
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