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 Cloneufc
Master Exploder!
Registered: 11/15/07
Posts: 1,234
Loc: Las Vegas 
Last seen: 4 years, 8 days
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 Making an isolate from a piece of rhizomorph? 
#8785854 - 08/17/08 10:48 AM (13 years, 5 months ago) |
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I start all my projects from jars. Then I go to bulk. I use nothing but hpoo in my jars to cut down on costs like verm. I put in some hpoo, use micropore tape on the lid, and pc it. When my poo cakes are done colonizing, I take the best strands of rhizomorphs off the cake with tweezers inside my glovebox. I put the rhizo's in sterilized seaweed and karo broth. I have had good results with LC's this way.
I hope this will minimize sectoring. I want to know if I can take the rhizomorphs off this way to cut down on the amount of transfers when I start messing with agar and petri dishes?
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
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Re: Making an isolate from a piece of rhizomorph? [Re: Cloneufc]
#8787055 - 08/17/08 04:31 PM (13 years, 5 months ago) |
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Just take the very tiny tippy tippiest tips from the rhizos, that helps minimize sectoring
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 http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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Cloneufc
Master Exploder!
Registered: 11/15/07
Posts: 1,234
Loc: Las Vegas
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Re: Making an isolate from a piece of rhizomorph? [Re: P.Menace]
#8799036 - 08/20/08 01:26 AM (13 years, 5 months ago) |
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Wouldnt it be easier to just take the mycelium found on a shroom cluster? Cluster as in multiple shrooms growing all hooked together. I had a cluster once with about ten shrooms all hooked together. Wouldnt this take the guess work out of finding a prolific fruiter and making a guaranteed fruiting sector? Can you take mycelium from this prolific fruit cluster and sector it even more? Would it even be worth it?
Edited by Cloneufc (08/20/08 03:58 AM)
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 BlimeyGrimey
Collector of Spores
Registered: 08/24/05
Posts: 3,770
Loc: Puget Sound
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Re: Making an isolate from a piece of rhizomorph? [Re: Cloneufc]
#8799628 - 08/20/08 06:26 AM (13 years, 5 months ago) |
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Cloning a cluster will more likely give you more sectors to deal with. It would be best to pick the best fruit and clone a piece of the inner stem. Take the tissue sample from an area away from the stem. This will guarantee you have a strain that will fruit on the substrate you are using and under the environmental conditions in your grow area.
Most clones of a fruit will be single sectors. You can still try to take a tip of rhizo mycelium and place it on agar but do as P.Menace said and take only a very tiny piece from the tip of the growth.
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Cloneufc
Master Exploder!
Registered: 11/15/07
Posts: 1,234
Loc: Las Vegas
Last seen: 4 years, 8 days
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Re: Making an isolate from a piece of rhizomorph? [Re: BlimeyGrimey]
#8802556 - 08/20/08 09:40 PM (13 years, 5 months ago) |
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RR said to use a piece no bigger than a grain of rice. After dabbling around with this stuff I can see how much multi spore sucks. I get way better pin sets and faster gorwing mycelium already just messing around on cardboard. Agar in on the way, just ordered some a few days ago from mycopath.
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
Last seen: 7 years, 10 months
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Re: Making an isolate from a piece of rhizomorph? [Re: Cloneufc]
#8803752 - 08/21/08 02:23 AM (13 years, 5 months ago) |
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Quote:
Cloneufc said:
Wouldnt it be easier to just take the mycelium found on a shroom cluster? Cluster as in multiple shrooms growing all hooked together. I had a cluster once with about ten shrooms all hooked together. Wouldnt this take the guess work out of finding a prolific fruiter and making a guaranteed fruiting sector? Can you take mycelium from this prolific fruit cluster and sector it even more? Would it even be worth it?
Well... however you want to come up to the point of "Isolating" since i believed by your first post you were already at this stage... wether its multispore or clone, it will sector, and all sectors must be tested for the characteristics wanted... ie. fast, rizo, growth, heavy pinset, fast pinset, co2 tollerence... and so on
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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