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OfflineNosLaser
Registered: 02/16/08
Posts: 61
Last seen: 3 years, 5 months
Colonization question
    #8802241 - 08/20/08 08:38 PM (9 years, 8 months ago)

I have 3 jars that have been colonizing for just under a month (PF Tek), came along really strong, with no contams.  They each have maybe a small triangle on the bottom of the jars that for some reason refuses to colonize.  I tried turning them upside down to see if it made a difference, and left them for about a week with no change in any of those particular spots. 

Those spots aside, they looked completely colonized to the point where if those spots didnt exist, the week I let them sit and monitored the changes could have been considered my week to consolidate upon the substrate. 

So being this is my first grow, I was a bit anxious to see what would happen if I birthed one.  I did it, and sure enough, it was completely colonized aside from that one spot which looked like the mycellium was ignoring it completely.  The un-colonized spot came off almost as easy as the dry verm layer.  So I birthed the other two with the same result.  I now have them soaking in cool water as part of the dunk and roll, so I will keep everyone posted on the progress. 

Is this common?  It really looked like the mycellium didnt want any part of those little spots for whatever reason, like it ignored them.  Did I birth them too early?  Thanks in advance.


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Offlinepftek
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Registered: 08/18/08
Posts: 832
Last seen: 7 years, 7 months
Re: Colonization question [Re: NosLaser]
    #8802532 - 08/20/08 09:36 PM (9 years, 8 months ago)

i don't know too much but did you perhaps burn the cakes?


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InvisibleKrackers
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Registered: 06/25/08
Posts: 36
Re: Colonization question [Re: pftek]
    #8802553 - 08/20/08 09:40 PM (9 years, 8 months ago)

hmm, i have a few resistant spots that have just been sitting uncolonized, though they are in no way perfect geometrical figures. So ill let em sit. Tell me how it works out.


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Colonization question [Re: Krackers]
    #8802593 - 08/20/08 09:48 PM (9 years, 8 months ago)

Those uncolonized parts probably had bacteria growing in that spot that prevented the mushroom mycelium.  It should be OK if it all washed off under the faucet prior to the dunk and roll.
RR


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OfflineNosLaser
Registered: 02/16/08
Posts: 61
Last seen: 3 years, 5 months
Re: Colonization question [Re: pftek]
    #8802597 - 08/20/08 09:50 PM (9 years, 8 months ago)

I keep them at a temperature controlled 79 degrees, so I don't think that's possible.  If it happened during sterilization, then I guess that could be possible, although I kept them on two set of jar lids away from the bottom with the water filled up to just the bottom of the jars.

Quote:

pftek said:
i don't know too much but did you perhaps burn the cakes?




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OfflineNosLaser
Registered: 02/16/08
Posts: 61
Last seen: 3 years, 5 months
Re: Colonization question [Re: RogerRabbit]
    #8802609 - 08/20/08 09:53 PM (9 years, 8 months ago)

Quote:

RogerRabbit said:
Those uncolonized parts probably had bacteria growing in that spot that prevented the mushroom mycelium.  It should be OK if it all washed off under the faucet prior to the dunk and roll.
RR




That sounds more likely.  The cakes are completely colonized otherwise, and these small parts just kinda came off under the steady stream of water and light pressure with my fingertip.  I was careful to not scratch the mycellium with my nail. 

Some people have suggested to cut the affected portions out based upon some reading/searching I have done.  Is that a good idea, or should I just leave them alone to do their thing naturally?


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