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Invisiblevampirism
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Registered: 03/14/04
Posts: 8,120
Post deleted by Moe Howard
    #880059 - 09/12/02 07:01 AM (21 years, 5 months ago)



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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,680
Loc: Alba
Re: FP spawn bags [Re: ]
    #880150 - 09/12/02 08:13 AM (21 years, 5 months ago)

>I dont see how opening the bag and dropping in a wedge of mycelium-colonized agar would keep contaminates out

you might be surprised....
since I started using agar I must have done 20-30 jars, without a flowhood/glovebox/"oven tek" or anything, and haven't had a single contam.
doesn't seem likely does it? maybe I've got magic hands or something:grin:
the normal way to do it is to autoclave the bags with the tops loosely held shut and let them cool in a flowhood or glovebox then innoculate with agar wedges and seal the bags, still in the glovebox.


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hmmm........


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Invisiblevampirism
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Post deleted by Moe Howard [Re: Champion des Champignons]
    #880164 - 09/12/02 08:19 AM (21 years, 5 months ago)



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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,680
Loc: Alba
Re: FP spawn bags [Re: ]
    #880195 - 09/12/02 08:41 AM (21 years, 5 months ago)

I'd say the "oven tek" is less than useless. If you don't have a flowhood or glovebox you want to minimise air movement, not get some nice convection currents to suck up dust etc and send it spinning around the kitchen:tongue:


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hmmm........


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Invisiblevampirism
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Post deleted by Moe Howard [Re: Champion des Champignons]
    #880208 - 09/12/02 08:51 AM (21 years, 5 months ago)



Edited by Morrowind (09/12/02 08:54 AM)


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Offlinezandorf
OTD residentvirginwizard-abstinenceain'teasy

Registered: 05/29/02
Posts: 3,072
Last seen: 20 years, 3 months
Re: FP spawn bags [Re: ]
    #880228 - 09/12/02 09:09 AM (21 years, 5 months ago)

see the thing about ovens is you can get them up to 400 degrees...trust me it'll be sterile after a half hour at 400. fuck bleach,


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We live in a world where lemonade is made with artificial flavours and furniture polish is made with all natural lemon.


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Invisiblevampirism
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Post deleted by Moe Howard [Re: zandorf]
    #880233 - 09/12/02 09:11 AM (21 years, 5 months ago)



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Invisibleshaggymane
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Registered: 03/11/02
Posts: 514
Loc: great white north
Re: FP spawn bags [Re: ]
    #880248 - 09/12/02 09:18 AM (21 years, 5 months ago)

use a tie wrap like form the hareware store fold the bag on its self and tie it


Edited by shaggymane (09/12/02 09:48 AM)


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Invisiblevampirism
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Post deleted by Moe Howard [Re: shaggymane]
    #880268 - 09/12/02 09:31 AM (21 years, 5 months ago)



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Invisibleshaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
Re: FP spawn bags [Re: ]
    #880289 - 09/12/02 09:51 AM (21 years, 5 months ago)

http://www.racerpartswholesale.com/tiewrap.htm
try this link so you know what they look like
Then hike down to the home depot
some times called cable ties or wraps


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Invisiblevampirism
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Post deleted by Moe Howard [Re: shaggymane]
    #880296 - 09/12/02 09:57 AM (21 years, 5 months ago)



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Invisibleshaggymane
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Registered: 03/11/02
Posts: 514
Loc: great white north
Re: FP spawn bags [Re: ]
    #880359 - 09/12/02 10:42 AM (21 years, 5 months ago)

keep the gun in your pants-pliers will pull it through


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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,680
Loc: Alba
Re: FP spawn bags [Re: ]
    #880482 - 09/12/02 11:39 AM (21 years, 5 months ago)

hmm, well I think the oven tek is more of a superstition thing than anything else.
plenty of people might get zero contams using the oven tek, but they probably would have without using it also.....
personally, I always wear black socks, and never get contams.
but to think that wearing black socks prevents contams is lacking logic  yes?:tongue:
or is it......? 


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hmmm........


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Offlinezeronio
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Registered: 10/16/01
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Loc: Slovenia
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Re: FP spawn bags [Re: Champion des Champignons]
    #881006 - 09/12/02 06:43 PM (21 years, 5 months ago)



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Invisiblevampirism
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Post deleted by Moe Howard [Re: shaggymane]
    #882094 - 09/13/02 12:07 PM (21 years, 5 months ago)



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Invisibleshaggymane
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Registered: 03/11/02
Posts: 514
Loc: great white north
Re: FP spawn bags [Re: ]
    #882211 - 09/13/02 01:16 PM (21 years, 5 months ago)

yes you got it ,but the tops of the bags will be bunched up-like a tit


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Invisiblezeta
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Registered: 05/24/02
Posts: 3,972
Re: FP spawn bags [Re: shaggymane]
    #883027 - 09/13/02 08:14 PM (21 years, 5 months ago)

Isn't it possible to seal the bags by putting a sheet of paper over it and ironing it?


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Invisiblevampirism
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Post deleted by Moe Howard [Re: shaggymane]
    #884857 - 09/16/02 04:01 AM (21 years, 5 months ago)



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OfflineAnnoA
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Re: FP spawn bags [Re: ]
    #884950 - 09/16/02 05:11 AM (21 years, 5 months ago)



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Invisiblevampirism
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Post deleted by Moe Howard [Re: Anno]
    #884954 - 09/16/02 05:13 AM (21 years, 5 months ago)



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OfflineAnnoA
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Re: FP spawn bags [Re: ]
    #884970 - 09/16/02 05:18 AM (21 years, 5 months ago)

don?t know.


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InvisibleDreaMaTrix
Shaman I am

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Registered: 02/11/02
Posts: 3,125
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Re: FP spawn bags [Re: ]
    #885209 - 09/16/02 07:17 AM (21 years, 5 months ago)

Yes, rubber bands work. I have used them on oven bags in the PC.
Use the wider, thicker ones :smile:

Good luck





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