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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Post deleted by Moe Howard
#880059 - 09/12/02 07:01 AM (22 years, 5 months ago) |
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Champion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,681
Loc: Alba
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Re: FP spawn bags [Re: ]
#880150 - 09/12/02 08:13 AM (22 years, 5 months ago) |
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>I dont see how opening the bag and dropping in a wedge of mycelium-colonized agar would keep contaminates out
you might be surprised.... since I started using agar I must have done 20-30 jars, without a flowhood/glovebox/"oven tek" or anything, and haven't had a single contam. doesn't seem likely does it? maybe I've got magic hands or something the normal way to do it is to autoclave the bags with the tops loosely held shut and let them cool in a flowhood or glovebox then innoculate with agar wedges and seal the bags, still in the glovebox.
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hmmm........
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Champion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,681
Loc: Alba
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Re: FP spawn bags [Re: ]
#880195 - 09/12/02 08:41 AM (22 years, 5 months ago) |
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I'd say the "oven tek" is less than useless. If you don't have a flowhood or glovebox you want to minimise air movement, not get some nice convection currents to suck up dust etc and send it spinning around the kitchen
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hmmm........
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Edited by Morrowind (09/12/02 08:54 AM)
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zandorf
OTD residentvirginwizard-abstinenceain'teasy

Registered: 05/29/02
Posts: 3,072
Last seen: 21 years, 2 months
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Re: FP spawn bags [Re: ]
#880228 - 09/12/02 09:09 AM (22 years, 5 months ago) |
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see the thing about ovens is you can get them up to 400 degrees...trust me it'll be sterile after a half hour at 400. fuck bleach,
-------------------- We live in a world where lemonade is made with artificial flavours and furniture polish is made with all natural lemon.
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Post deleted by Moe Howard [Re: zandorf]
#880233 - 09/12/02 09:11 AM (22 years, 5 months ago) |
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shaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
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Re: FP spawn bags [Re: ]
#880248 - 09/12/02 09:18 AM (22 years, 5 months ago) |
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use a tie wrap like form the hareware store fold the bag on its self and tie it
Edited by shaggymane (09/12/02 09:48 AM)
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Post deleted by Moe Howard [Re: shaggymane]
#880268 - 09/12/02 09:31 AM (22 years, 5 months ago) |
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shaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
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Re: FP spawn bags [Re: ]
#880289 - 09/12/02 09:51 AM (22 years, 5 months ago) |
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http://www.racerpartswholesale.com/tiewrap.htm try this link so you know what they look like Then hike down to the home depot some times called cable ties or wraps
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Post deleted by Moe Howard [Re: shaggymane]
#880296 - 09/12/02 09:57 AM (22 years, 5 months ago) |
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shaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
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Re: FP spawn bags [Re: ]
#880359 - 09/12/02 10:42 AM (22 years, 5 months ago) |
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keep the gun in your pants-pliers will pull it through
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Champion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,681
Loc: Alba
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Re: FP spawn bags [Re: ]
#880482 - 09/12/02 11:39 AM (22 years, 5 months ago) |
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hmm, well I think the oven tek is more of a superstition thing than anything else. plenty of people might get zero contams using the oven tek, but they probably would have without using it also..... personally, I always wear black socks, and never get contams. but to think that wearing black socks prevents contams is lacking logic yes? or is it......?
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hmmm........
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zeronio
Stranger


Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 3 days, 20 hours
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Post deleted by Moe Howard [Re: shaggymane]
#882094 - 09/13/02 12:07 PM (22 years, 5 months ago) |
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shaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
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Re: FP spawn bags [Re: ]
#882211 - 09/13/02 01:16 PM (22 years, 5 months ago) |
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yes you got it ,but the tops of the bags will be bunched up-like a tit
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zeta
Stranger

Registered: 05/24/02
Posts: 3,972
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Re: FP spawn bags [Re: shaggymane]
#883027 - 09/13/02 08:14 PM (22 years, 5 months ago) |
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Isn't it possible to seal the bags by putting a sheet of paper over it and ironing it?
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Post deleted by Moe Howard [Re: shaggymane]
#884857 - 09/16/02 04:01 AM (22 years, 5 months ago) |
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 6 days, 4 hours
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Re: FP spawn bags [Re: ]
#884950 - 09/16/02 05:11 AM (22 years, 5 months ago) |
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vampirism
Stranger


Registered: 03/14/04
Posts: 8,120
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Post deleted by Moe Howard [Re: Anno]
#884954 - 09/16/02 05:13 AM (22 years, 5 months ago) |
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