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Amazon Shop: ½ Pint Jars, Perlite, Vermiculite

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OfflineFloat
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Unintentionally fruiting in vitro, any advice?
    #8794758 - 08/19/08 11:32 AM (9 years, 6 months ago)

Things were going well enough for a first grow, none of my jars (5 1/2pint PF-TEK) got contam, they colonized at a decent rate and all at the same time. But it seems Ive run into a bit of an issue. At the final stages of colonization the jars started pinning, outside colonization of the bottoms of the jars seemingly halted (98%), and pins turned to fruits.

The original plan was to use a Shotgun FC after a dunk and probably a roll (verm might be dirty, no oven), and let nature take its course. Things went way faster than intended. The FC isnt done and Im short on supplies and equipment (drill and I might need more perlite).

So here I am unprepared and unsure what to do. Should I just let them fruit in vitro instead of taking the risk of possibly ruining almost a month of work?

If I birth them, should I pick the pins and fruits off? Some of these look like they have potential. Cauterizing the lightly colonized spots might be my only chance at a good harvest, but that makes me nervous.

A smaller harvest is better than no harvest!

These are the jars.



Thanks everyone.


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Offlinegrod31
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Re: Unintentionally fruiting in vitro, any advice? [Re: Float]
    #8794766 - 08/19/08 11:35 AM (9 years, 6 months ago)

if you don't want that to happen just stop it.  open the jars pull them off ( i guess you can leave them on i don't see real harm in that) and fruit the jars the way they are supposed to be fruited.


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
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seconds and they'd make me explain
VVVVV Free Myco Thread


Edited by grod31 (08/19/08 11:38 AM)


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InvisibleMisterMuscaria
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Re: Unintentionally fruiting in vitro, any advice? *DELETED* [Re: grod31]
    #8794791 - 08/19/08 11:40 AM (9 years, 6 months ago)

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Offlinegrod31
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Re: Unintentionally fruiting in vitro, any advice? [Re: MisterMuscaria]
    #8794828 - 08/19/08 11:47 AM (9 years, 6 months ago)

Quote:

MisterMuscaria said:
I ALWAYS let mine fruit in vitro before birthing, to ensure full colonization.



hmm i guess thats really safe but couldn't that take for ever sometimes?


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


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OfflineRogerRabbitM
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Re: Unintentionally fruiting in vitro, any advice? [Re: grod31]
    #8794847 - 08/19/08 11:51 AM (9 years, 6 months ago)

That often happens when the inoculation/gas exchange holes are drilled too large.  In addition, some strains simply want to pin at full colonization.

Personally, I'd let those finish invitro, but if you want to birth them, be gentle to avoid aborts, and keep them at near saturation humidity.  With a dunk, they should be ready to pick within two to three days.

I'm surprised nobody has repeated the myth that 'incubation in total darkness' will prevent that. :laugh:
RR


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OfflineUncleMike
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Re: Unintentionally fruiting in vitro, any advice? [Re: RogerRabbit]
    #8794897 - 08/19/08 12:00 PM (9 years, 6 months ago)

Quote:

RogerRabbit said:
That often happens when the inoculation/gas exchange holes are drilled too large.  In addition, some strains simply want to pin at full colonization.

Personally, I'd let those finish invitro, but if you want to birth them, be gentle to avoid aborts, and keep them at near saturation humidity.  With a dunk, they should be ready to pick within two to three days.

I'm surprised nobody has repeated the myth that 'incubation in total darkness' will prevent that. :laugh:
RR




Right...I have a friend who has had those fuckers pin in-vitro in the dark many times....that was back in his cake days though...


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Live each day like it will be your last, tomorrow my never come.
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Offlinegrod31
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Re: Unintentionally fruiting in vitro, any advice? [Re: RogerRabbit]
    #8794962 - 08/19/08 12:09 PM (9 years, 6 months ago)

Quote:

RogerRabbit said:
  In addition, some strains simply want to pin at full colonization.
RR



you do mean sub strains right? you advocate a cube is a cube is a cube quite often, are you telling me there is a specific cube strain that likes to fruit invitro ?


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineUncleMike
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Re: Unintentionally fruiting in vitro, any advice? [Re: grod31]
    #8794980 - 08/19/08 12:13 PM (9 years, 6 months ago)

PF classic (Matias Romero)(sp?) love to fruit in-vitro! But a cube is a cube is a cube...it is like this...we are all of the human race but we all look different and do different things like some have premature ejaculations and some don't...but because of these subtle differences does not qualify use for science to denote us a latin name or species...get it?


--------------------
Live each day like it will be your last, tomorrow my never come.
SporeSmart


Edited by UncleMike (08/19/08 12:14 PM)


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OfflineFloat
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Re: Unintentionally fruiting in vitro, any advice? [Re: grod31]
    #8795114 - 08/19/08 12:43 PM (9 years, 6 months ago)

I'm relieved, to say the least. They do seem to be doing just fine in the jar, as opposed to the cake that I "birthed" yesterday. The birthed cake doesn't look any different than it did yesterday, but no sign of contam (yet).

The strain is Texas Goliad, Ralphster's says it's great with in vitro, and Lil' Shop says "It has a tendency to fruit through the casing layer and not fully colonize it like many strains. It shows pronounced bluing and is a great beginner strain." The bluing really is strong. My syringe came from Lil' Shop.

The birthed cake:


Ok, so I do kinda have an FC but it might not be viable for several reasons:

Being the overenthusiastic and uninformed beginner that I was a while ago, I bought Miracle-Gro Perlite (w/ the plant nutes in it)which I figure might damage a cake if they come in direct contact. Too many variables I don't feel like playing around with on a first grow. I had to rinse the perlite in a series of garbage bags due to not having a sink or a good strainer, real McGuyver shit. Needless to say, perlite is a pain in the ass for me.

The only drill I have is this little crappy 6V (not mine) that would take forever to make all the holes in the Shotgun FC. Kinda strapped for cash so buying a better one is iffy; I guess I could punch holes with a nail, we'll see.

These are the fruits I picked off the birthed cake yesterday, the ones that grew through the verm layer.


Appreciate all the input guys, everyday I learn so much here.


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OfflineZev
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Re: Unintentionally fruiting in vitro, any advice? *DELETED* [Re: RogerRabbit]
    #13969200 - 02/15/11 07:58 PM (7 years, 8 days ago)

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Invisibleslapphappypill
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Re: Unintentionally fruiting in vitro, any advice? [Re: Zev]
    #13969368 - 02/15/11 08:27 PM (7 years, 8 days ago)

Birth, dunk, roll, fruit!

Finishing in-vitro just means to fruit out of the jar.

it's diffinently ok to D&R cakes like this!


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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OfflineZev
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Re: Unintentionally fruiting in vitro, any advice? *DELETED* [Re: slapphappypill]
    #13969422 - 02/15/11 08:35 PM (7 years, 8 days ago)

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Invisibleslapphappypill
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Re: Unintentionally fruiting in vitro, any advice? [Re: Zev]
    #13969653 - 02/15/11 09:18 PM (7 years, 8 days ago)

you don't have to pick off the pins, try to keep them on as that will better the first flush.

As far as fruiting in the jar, you gotta birth the cake, then dunk it, then place it back in the jar (No roll) Add top layer verm if you want extra water  for the cake :bigjoint:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Perlite, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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