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InvisibleAdoreChampignons
Mycophilic One

Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
Does Isolation On Gentamicin Agar Injure Isolates?
    #8780330 - 08/16/08 12:13 AM (15 years, 6 months ago)

Ive read in a couple places that discouraged the isolation of mushroom cultures on gentamicin enriched agar.  The reason given for avoiding its use was that while gentamicin does kill bacterial contaminants, its rumored that it also damages the chromosomes of the mushroom isolates.  I do know that most living organism have reparative mechanisms, but I wondered if the use of gentamicin agar is really that problematic.  Could it be just my imagination and the power of suggestion?  The mushroom cultures that I were able to isolate on gentamicin agar do appear less vigorous than cultures isolated on regular MEA.

What do you think?  Whats your opinion of the use of gentamicin enriched agar?


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There is no such thing as a dumb question.  There are just curious people trying to learn something new. 

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OfflineMycoAu
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Registered: 07/18/07
Posts: 1,047
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Re: Does Isolation On Gentamicin Agar Injure Isolates? [Re: AdoreChampignons]
    #8780373 - 08/16/08 12:19 AM (15 years, 6 months ago)

Use antibiotic agar only as a "last" resort.  If you've tried to isolate a given strain or species repeated without success on regular agar formulas, then try antiobiotic media.  Otherwise, try to stick with regular formulas.  The only time I wouldn't try regular formulas is when dealing with a species/specimen you aren't likely to find/obtain again.  Then, you should do all that's in your power to obtain a clean sample/isolate. (which usually includes the use of antibiotics)

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InvisibleAdoreChampignons
Mycophilic One

Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
Re: Does Isolation On Gentamicin Agar Injure Isolates? [Re: MycoAu]
    #8780471 - 08/16/08 12:48 AM (15 years, 6 months ago)

I see your point. Thanks.


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There is no such thing as a dumb question.  There are just curious people trying to learn something new. 

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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Does Isolation On Gentamicin Agar Injure Isolates? [Re: AdoreChampignons]
    #8782884 - 08/16/08 04:00 PM (15 years, 6 months ago)

I use antibiotic agar as a matter of course before transferring to master culture slants. I don't want any hidden bacteria going into long term storage with my cultures. I also use it as a matter of course when working with any outdoor fruits that I've cloned.  I have never noticed any genetic problems, and mycelium grown on gentamicin sulfate agar fruits normally.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineMycoAu
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Re: Does Isolation On Gentamicin Agar Injure Isolates? [Re: RogerRabbit]
    #8783175 - 08/16/08 05:39 PM (15 years, 6 months ago)

RR, which antibiotics do you use?  Gentamicin?  Chloramphenicol?  Others? Combinations?

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OfflineP.Menace
Contradicting the Status Quo
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Re: Does Isolation On Gentamicin Agar Injure Isolates? [Re: MycoAu]
    #8785370 - 08/17/08 03:55 AM (15 years, 6 months ago)

Gentamicin Sulfate has never done me wrong...
but like RR stated, only if I think i need it or right before culture storage


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roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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InvisibleAdoreChampignons
Mycophilic One

Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
Re: Does Isolation On Gentamicin Agar Injure Isolates? [Re: P.Menace]
    #8786029 - 08/17/08 10:03 AM (15 years, 6 months ago)

Thanks again for all you comments.  I really do appreciate hearing form those that have a lot more experience than myself. 
Be Well


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There is no such thing as a dumb question.  There are just curious people trying to learn something new. 

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Offlinex7x_x7x
x7x, my problem child.
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Re: Does Isolation On Gentamicin Agar Injure Isolates? [Re: AdoreChampignons]
    #8786056 - 08/17/08 10:13 AM (15 years, 6 months ago)

genta and clora are being used for decades in medical mycology; i never read something about genetic damage.


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koh samui and oak ridge are my favourite strains

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