Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinefunkyballoon
Pooh-Bah
Registered: 01/18/99
Posts: 311
Last seen: 22 years, 6 months
Re: Question on agar culture
    #87081 - 08/01/00 11:15 AM (23 years, 7 months ago)

Either the culture should be completely sealed, or it should be in some sort of sterile humid enviornment; otherwise it will dry out and get contaminated. Spores should germinate within a few days.

Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87083 - 08/03/00 02:07 PM (23 years, 7 months ago)

It?s a good idea to seal your agar containers with a cut piece of saran wrap like on picture. This way gases can exchange but no contaminants.

Extras: Filter Print Post Top
OfflineB.I.O.
old hand
Male User Gallery
Registered: 05/26/99
Posts: 458
Last seen: 1 year, 1 month
Re: Question on agar culture [Re: funkyballoon]
    #87084 - 08/03/00 02:45 PM (23 years, 7 months ago)

supcoolperfect,,,,,

BiO.


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87085 - 08/03/00 02:47 PM (23 years, 7 months ago)

BIO, sag hast, Du denn das noch nicht gekannt?

Extras: Filter Print Post Top
Anonymous

Re: Question on agar culture [Re: funkyballoon]
    #87086 - 08/04/00 02:39 AM (23 years, 7 months ago)

dudes.. point me to some articles about the agar tek please.
and where do you get the agar?
best,
A

Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87087 - 08/04/00 05:08 AM (23 years, 7 months ago)

A nice article on getting started: http://www.mykoweb.com/cultivation.html

Extras: Filter Print Post Top
InvisiblePinhead
Oregano
Male

Registered: 05/13/00
Posts: 1,819
Loc: Hootersville
Re: Question on agar culture [Re: funkyballoon]
    #87088 - 08/08/00 08:06 AM (23 years, 7 months ago)

I've tried starting an agar culture with spores that I know are viable,after a week..still nothing. My jars were sealed up air tight.Maybe I should try the saran wrap thing or get some petri dishes instead of my pyrex bowls.
I guess this proves that mycelium needs at least some air circulation,unless theres' some other variable I'm not taking into consideration.
Anyone else have any other thoughts on this?

Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87089 - 08/08/00 08:25 AM (23 years, 7 months ago)

Yes, you must not seal the petris compleately, they need some gas exchange. Seal them with saran wrap.

Extras: Filter Print Post Top
Anonymous

Re: Question on agar culture [Re: funkyballoon]
    #87090 - 08/08/00 12:12 PM (23 years, 7 months ago)

So, I take the seal of the Saran Wrap is just imperfect enough to not be airtight? How did you cut the whole roll of Saran Wrap?

------------------
"Not all drugs are good... some are great."


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87091 - 08/08/00 12:48 PM (23 years, 7 months ago)

When you seal the petry dish, strech the saran a little, it will then take the form of the petri and seal it very tight.
Polyethilene is tight against water and small particles but leaves gases through.

I cut the piece off with a sharp knive. Watch your fingers!


Extras: Filter Print Post Top
Anonymous

Re: Question on agar culture [Re: funkyballoon]
    #87092 - 08/08/00 07:50 PM (23 years, 7 months ago)

saran wrap does not let air through maybey it gets through from the space thats not coverd

Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87093 - 08/09/00 12:55 AM (23 years, 7 months ago)

doublepost

[This message has been edited by Anno (edited August 09, 2000).]


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87094 - 08/09/00 01:20 AM (23 years, 7 months ago)

quote:
Originally posted by psyco:
saran wrap does not let air through...

Oh, it does, it does. Not much, but it does.

The whole side of the petridish is supposed to be sealed, so the whole thing is dust tight but not gas tight.


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87095 - 08/09/00 03:29 AM (23 years, 7 months ago)

I found that I might clear some things up.
The plastic foil I use to seal petri dishes IS NOT the original Saran Wrap tm. it is a thin plastic foil made of PE(poly ethylene).
Poly ethylene leaves gases through.

Saran Wrap on the other side is made from PVDC(polyvinylidene chloride).
The source says that ...The result is a barrier against oxygen, moisture, chemicals and heat-qualities used to protect food, consumer and industrial products.....

So it might realy be that the original Saran Wrap won?t work.

Try finding some foil made of PE.

More info on Saran Wrap.


Extras: Filter Print Post Top
InvisibleNatura
COSMIC-REPORTER

Registered: 07/19/00
Posts: 31
Loc: EU
Re: Question on agar culture [Re: funkyballoon]
    #87096 - 08/09/00 11:21 AM (23 years, 7 months ago)

I bought a roll of Para film (2 inchs large) from Fungi.com-(elastic wax like film). It works well. I can't remember the price, it should be $25-30 for 250ft. About Gas exchange in petri for Mycelium growing is not sure to need. I recommend wrap it tight with film anyway, if U keep them in unstelirized condition.

Natura.



--------------------
N+

Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87097 - 08/09/00 12:11 PM (23 years, 7 months ago)

Natura: What do you mean with ...if U keep them in unstelirized condition...?

Extras: Filter Print Post Top
Offlinekeyzar
journeyman
Registered: 04/03/00
Posts: 56
Last seen: 22 years, 11 days
Re: Question on agar culture [Re: funkyballoon]
    #87098 - 08/09/00 05:39 PM (23 years, 7 months ago)

i imagine natura means that if you dont have them in a glove box or sterile incubation chamber of sorts

Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 51 minutes
Re: Question on agar culture [Re: funkyballoon]
    #87099 - 08/09/00 09:33 PM (23 years, 7 months ago)

Aha...
In non sterile enviroment...

Extras: Filter Print Post Top
OfflineKrupa
veteran
Registered: 01/08/00
Posts: 443
Last seen: 22 years, 10 months
Re: Question on agar culture [Re: funkyballoon]
    #87100 - 08/11/00 02:22 AM (23 years, 7 months ago)

I've heard good things about using electricians tape as a dish wrapping. Just a thought.

Extras: Filter Print Post Top
Offlinemajishroomer
newbie
Registered: 08/10/00
Posts: 2
Last seen: 22 years, 4 months
Re: Question on agar culture [Re: funkyballoon]
    #87101 - 08/12/00 10:53 AM (23 years, 7 months ago)

i use the baby bottle for agar stabs.cut the nipple tip off and stuff with aquarium filter floss or cotton.this allows for ventilation and maintains sterility.plus spores can be injected through nipple.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* Agar Culture transfer to Liquid Culture Tek Compiler 25,371 11 06/27/03 05:11 PM
by Compiler
* Starting Agar Culture. Please Help. the_guy 1,140 1 07/25/01 09:08 PM
by zetek
* Re: Dehydration of Agar Cultures? Anonymous 1,944 7 02/09/00 05:34 PM
by Odin 13
* Cloning:9er Tek vs. Agar cultures meatman 2,497 2 09/18/02 01:16 PM
by meatman
* Re: How many FOAF use Agar culture, SURVEY? ALDER_057 2,834 15 04/24/01 09:29 AM
by Anonymous
* Re: a few questions for an agar guru... gray1 3,307 8 05/06/01 03:18 PM
by gratefulredhead
* bohemica pinheads on agar Una 5,813 15 06/18/01 05:55 PM
by amanita
* HAHAHAHA Agar You guys are really funny!!! CultyVader 1,150 7 03/22/03 10:40 PM
by Baby_Hitler

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
3,088 topic views. 1 members, 2 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.004 seconds on 12 queries.