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OfflineLarrythescaryrexS
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question as to how one inculates without verm layr
    #870654 - 09/08/02 11:35 PM (14 years, 6 months ago)

When one is using grain and has not verm layer, one takes the lid off to inculate correct?


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RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


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Invisiblematts
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[Re: Larrythescaryrex]
    #870699 - 09/09/02 12:07 AM (14 years, 6 months ago)



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OfflineLarrythescaryrexS
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Re: question as to how one inculates without verm layr [Re: Larrythescaryrex]
    #870719 - 09/09/02 12:20 AM (14 years, 6 months ago)

yeah, thats what I thought, but I was hoping you could innoculate through a polyfil filter or something..


--------------------
RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


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OfflineShaw
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Re: question as to how one inculates without verm layr [Re: Larrythescaryrex]
    #870787 - 09/09/02 12:49 AM (14 years, 6 months ago)

i unscrew the filter and squirt under it. i had 1 of 10 comtam last time. If you use polly-fill in a medal lid, you can punch a hole in the other side of the lid and tape it off. I havn't had any contams this way. I have no flow hood of glove box or anything. i do have a hepa for the room though. and a spray a little bleach.


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Invisiblematts
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[Re: Shaw]
    #870873 - 09/09/02 01:35 AM (14 years, 6 months ago)



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OfflineShaw
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Re: question as to how one inculates without verm layr [Re: matts]
    #871098 - 09/09/02 03:54 AM (14 years, 6 months ago)

could be. I also use the fp/mycosupply disks. they are stiffer and the exact right size so they stay on the ring. makes it a little easier. I still like the polyfill method though. less contam worries.


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Edited by Shaw (09/09/02 03:55 AM)


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Offlinemntlfngrs
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Re: question as to how one inculates without verm layr [Re: Larrythescaryrex]
    #871106 - 09/09/02 03:59 AM (14 years, 6 months ago)

I've been stretching balloons over the lids. Makes a seal like the ones on the vials doctors draw shots from. Seals when you pull out.


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OfflineShaw
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Re: question as to how one inculates without verm layr [Re: mntlfngrs]
    #871132 - 09/09/02 04:24 AM (14 years, 6 months ago)

but how is the balloon steril?


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Invisiblemickey_rourke
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Re: question as to how one inculates without verm layr [Re: mntlfngrs]
    #872248 - 09/09/02 12:16 PM (14 years, 6 months ago)

In reply to:

I've been stretching balloons over the lids. Makes a seal like the ones on the vials doctors draw shots from. Seals when you pull out.



In my experience, the hole left after puncturing stretched rubber most certainly is not sealed. My observations indicate that the hole usually grows due to the forces generated by stretching the rubber.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Invisiblerobinhood2
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Re: question as to how one inculates without verm layr [Re: Larrythescaryrex]
    #873148 - 09/09/02 08:20 PM (14 years, 6 months ago)

I have a friend who uses the polyfill lid method. One 1/4" hole in the middle of the lid has polyfill in it. Another 1/8" hole is drilled farther away from the middle, and covered with electrical tape during pressure cooking. He's found the electrical tape adheres well to the lid and holds up like a champ during pressure cooking.

For innoculation, he is there with syinge, electrical tape, scissors and alcohol flame. The sterilized needle is poked right through the taped-over hole and innoculates. Upon pulling the needle from the hole, he immediately re-covers it with another piece of electrical tape. VOILA!


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"Rob? That's a naughty word, we never rob. We just.. borrow a bit from those who can afford it."


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Invisiblemickey_rourke
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Re: question as to how one inculates without verm layr [Re: robinhood2]
    #873175 - 09/09/02 08:34 PM (14 years, 6 months ago)

Why doesn't he just innoculate through the polyfill hole? I find it to be very self sealing.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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OfflineLarrythescaryrexS
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Re: question as to how one inculates without verm layr [Re: mickey_rourke]
    #873183 - 09/09/02 08:42 PM (14 years, 6 months ago)

CAN you inoculate through the polyfil?


--------------------
RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemickey_rourke
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Re: question as to how one inculates without verm layr [Re: Larrythescaryrex]
    #873188 - 09/09/02 08:43 PM (14 years, 6 months ago)

I always do and I never have contamination from innoculation except when I have a bad syringe.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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InvisibleRoadkill
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Re: question as to how one inculates without verm layr [Re: Larrythescaryrex]
    #873205 - 09/09/02 08:54 PM (14 years, 6 months ago)

In reply to:

CAN you inoculate through the polyfil?




Yeppers!



--------------------
Laterz, Road

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You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinemntlfngrs
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Re: question as to how one inculates without verm layr [Re: mickey_rourke]
    #875497 - 09/10/02 08:04 PM (14 years, 6 months ago)

The ballon is on there in such a way that the top is loose, only streach is aroud the ring. The balloon is washed and then sterilized with the jars. I don't know if it would hold up in a PC. I only had one hole in the center of the ballon and streched it to the 4 holes. Works for me but where I am there isn't alot of trouble with contams. Too dry outside.


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Be all and you'll be to end all


Edited by mntlfngrs (09/10/02 08:30 PM)


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Offlinebluepoo
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Re: question as to how one inculates without verm layr [Re: Larrythescaryrex]
    #875661 - 09/10/02 09:19 PM (14 years, 6 months ago)

cut a small hole in the lid and fill with silicone selent then use this it seels when you pull out if you use enouhf silicone


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the above is what the litel leprecans told me to say and should be regarded as such
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Invisiblemickey_rourke
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Re: question as to how one inculates without verm layr [Re: bluepoo]
    #875665 - 09/10/02 09:22 PM (14 years, 6 months ago)

I have a feeling my 16 ga. needles would take a core sample.. :grin: 


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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OfflineDERRAYLD
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Re: question as to how one inculates without verm layr [Re: mickey_rourke]
    #875746 - 09/10/02 10:06 PM (14 years, 6 months ago)

Whatever happened to the concept of four innoculation holes in the lid??
Innocultae and cover the holes with tape.
Sure as hell woroks for me.


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Derrayld and out of control


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Invisiblemickey_rourke
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Re: question as to how one inculates without verm layr [Re: DERRAYLD]
    #875747 - 09/10/02 10:07 PM (14 years, 6 months ago)

In reply to:

Whatever happened to the concept of four innoculation holes in the lid??



We're talking about shakeable grain, not PF jars..


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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OfflineDERRAYLD
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Re: question as to how one inculates without verm layr [Re: mickey_rourke]
    #875754 - 09/10/02 10:13 PM (14 years, 6 months ago)

What? you assume I`m talking about pf jars??
I`ve never had a problem with grains and innoculating through the lid.


--------------------
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