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Wonderboyz
Lysol King


Registered: 07/27/08
Posts: 19
Last seen: 16 years, 5 months
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big inoculation problem
#8701313 - 07/30/08 09:13 AM (16 years, 5 months ago) |
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so i got all my jars in my glove box...go to inoculate...and my inoculation holes are not big enof!!! wtf... how do i go about fixing this problem...i cant resteam these jars, im not off by a hole lot but ive been trying to be very contam free and now what?! take a hammer and nail back to them?
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beengonetoolong
Gravy Train Conductor



Registered: 06/01/08
Posts: 523
Last seen: 14 years, 7 months
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Re: big inoculation problem [Re: Wonderboyz]
#8701314 - 07/30/08 09:14 AM (16 years, 5 months ago) |
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i've had this happen before, better be very careful and bore the holes out a little bit more with a larger nail. =/
The time I had it happen I had no problems, all colonized fine, but it still sucks.
--------------------
 
Is there life after death? Trespass and find out.
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Wonderboyz
Lysol King


Registered: 07/27/08
Posts: 19
Last seen: 16 years, 5 months
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should i take the lid off?! or hammer down into...im using PF tek so i have verm layer on top
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chiz
Stranger
Registered: 07/17/08
Posts: 67
Last seen: 16 years, 4 months
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Re: big inoculation problem [Re: Wonderboyz]
#8701340 - 07/30/08 09:24 AM (16 years, 5 months ago) |
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If it were me I would get a bigger nail and flame (and/or) wipe it down with alcohol in between jars.
and yeah definitely leave the lids on there
Edited by chiz (07/30/08 09:24 AM)
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robanero
β Lib


Registered: 09/05/06
Posts: 874
Loc: Somewhere in Tx
Last seen: 6 years, 8 months
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Re: big inoculation problem [Re: chiz]
#8701435 - 07/30/08 09:57 AM (16 years, 5 months ago) |
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Don't use a hammer just twist the nail and work it throgh the hole.
--------------------
 
310 open cap Hawaiians grown a 1 pint WBSF cake.
SPAWN RATIO CALCULATOR
My Little Hawaiians
Spawn Bag Tek
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Wonderboyz
Lysol King


Registered: 07/27/08
Posts: 19
Last seen: 16 years, 5 months
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Re: big inoculation problem [Re: robanero]
#8701448 - 07/30/08 10:02 AM (16 years, 5 months ago) |
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too late, i lightly tapped the hammer to make the holes bigger. it worked fine i suppose and i flamed/wiped down nail with alcohol. strange thing my first time inoculating and when i flamed the needle with candle...it started to like shoot out the end of the syringe. i think it could have been air, but it was hard to read how much i was inoculating since the syringe had a big air bubble in it, but i shook it well and inoculated all 4 areas. I lyosol'd the fk out of the area about 3 times b4 i inoculated, and when i tried to fit my syringe in glove box it was too small, so i put the jars down inside the lid but left it open and did my work that way. im hoping i don't get contams, if i do i will know next time to be better prepared with my glove box. but other than that i was sterile.
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robanero
β Lib


Registered: 09/05/06
Posts: 874
Loc: Somewhere in Tx
Last seen: 6 years, 8 months
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Re: big inoculation problem [Re: Wonderboyz]
#8703278 - 07/30/08 04:45 PM (16 years, 5 months ago) |
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I DO NOT RECOMMEND THIS IN ANYWAY!!!! I don't even use a glove box to noc up my jars I flame before the first hole of the jar and wipe with 70% iso between each hole and i never get contams in my pf jars. You should be fine. I would not use a candle to flame. You can make an alcohol lamp very easily with a small jar with cotton wick that you make with cotton balls.
--------------------
 
310 open cap Hawaiians grown a 1 pint WBSF cake.
SPAWN RATIO CALCULATOR
My Little Hawaiians
Spawn Bag Tek
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aaronemerritt
boomer groomer


Registered: 07/28/08
Posts: 330
Last seen: 3 years, 1 month
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Re: big inoculation problem [Re: robanero]
#8703362 - 07/30/08 05:03 PM (16 years, 5 months ago) |
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I had this happen but my needle was pretty stout so i just used a little extra force and shoved it in the hole
-------------------- Mushrooms are a funny drug!!!!!!!!!!!!!
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