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OfflineWonderboyz
Lysol King


Registered: 07/27/08
Posts: 19
Last seen: 16 years, 5 months
sterile glovebox
    #8699202 - 07/29/08 07:59 PM (16 years, 5 months ago)

So steamed all 5 of my jars using PF. And soon I will be to the inoculation process, I made this ghetto rigged up glovebox that Im wondering will get the job done.
I used:

1 box(not brand new)
1 Pie cover
Liberal amounts of tape
Lysold the fk out of the inside.

Heres are some pics:






Beautiful eh?

thoughts, comments, think it will work?

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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: sterile glovebox [Re: Wonderboyz]
    #8699212 - 07/29/08 08:02 PM (16 years, 5 months ago)

where do your hands go?

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OfflineSnate
Stranger
Registered: 07/24/08
Posts: 30
Last seen: 15 years, 9 months
Re: sterile glovebox [Re: Wonderboyz]
    #8699217 - 07/29/08 08:03 PM (16 years, 5 months ago)

It just needs to stop air flow. So unless there is a fan directly above you, you'll be fine.

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OfflinePsuper
Psilocybin


Registered: 02/12/08
Posts: 2,878
Loc: PNW
Last seen: 1 month, 1 day
Re: sterile glovebox [Re: Wonderboyz]
    #8699225 - 07/29/08 08:05 PM (16 years, 5 months ago)

Sure, but don't introduce a flame anywhere near that thing after spraying it with Lysol.  When you're ready to work you can spray the air inside with plain water (as RR recommends) to help settle any contaminants.  Remember, the purpose of a glovebox isn't to make a perfectly sterile environment; instead it is a still air space to work inside of.  Where are your arm holes though??? ~Pixie~


--------------------
Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk

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OfflineWonderboyz
Lysol King


Registered: 07/27/08
Posts: 19
Last seen: 16 years, 5 months
Re: sterile glovebox [Re: Psuper]
    #8699232 - 07/29/08 08:07 PM (16 years, 5 months ago)

oh, the holes for the hands will be made tomorrow :smile: I thought that keeping it closed would be more sterile

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OfflineJeets
Stranger


Registered: 07/06/08
Posts: 258
Last seen: 15 years, 2 months
Re: sterile glovebox [Re: Wonderboyz]
    #8699235 - 07/29/08 08:08 PM (16 years, 5 months ago)

Ive never used a box and have yet to see a contamination. It looks nice though.

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Offlineskaterickets
Musician
Male User Gallery


Registered: 06/26/08
Posts: 653
Last seen: 10 years, 6 months
Re: sterile glovebox [Re: Wonderboyz]
    #8699449 - 07/29/08 08:58 PM (16 years, 5 months ago)

Efficient...
Inexpensive...
:thumbup:


--------------------

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OfflineWonderboyz
Lysol King


Registered: 07/27/08
Posts: 19
Last seen: 16 years, 5 months
Re: sterile glovebox [Re: Wonderboyz]
    #8699543 - 07/29/08 09:15 PM (16 years, 5 months ago)

Really, but wouldn't making the glove box sterile get rid of contaminants completely, rather than settle them. or is than an advantage to settling contaminants?

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Offlineskaterickets
Musician
Male User Gallery


Registered: 06/26/08
Posts: 653
Last seen: 10 years, 6 months
Re: sterile glovebox [Re: Wonderboyz]
    #8699591 - 07/29/08 09:24 PM (16 years, 5 months ago)

Quote:

Wonderboyz said:
Really, but wouldn't making the glove box sterile get rid of contaminants completely, rather than settle them. or is than an advantage to settling contaminants?




Assuming that what I thought you meant is what you actually meant, it's really hard to get rid of completely all containment but with a glove box you get rid of a very high amount of them making it a lot better than inoculating in open air.
Make sure to bleach spray the box once it is made, and then every time you inoculate spray with Lysol before use.
The purpose of a glove box is to sterilize an area for inoculation and then keep fresh un-sterilized air out of the sterile area, in order to keep the area sterilized for longer periods of time.

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OfflineWonderboyz
Lysol King


Registered: 07/27/08
Posts: 19
Last seen: 16 years, 5 months
Re: sterile glovebox [Re: Psuper]
    #8699687 - 07/29/08 09:44 PM (16 years, 5 months ago)

Quote:

Psuper said:
  When you're ready to work you can spray the air inside with plain water (as RR recommends) to help settle any contaminants. 




Vs. killing contaminants?

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OfflineGroomies
Ghost
 User Gallery


Registered: 08/16/07
Posts: 1,119
Last seen: 8 years, 1 month
Re: sterile glovebox [Re: Wonderboyz]
    #8699843 - 07/29/08 10:23 PM (16 years, 5 months ago)

just be careful of glovebox fire.  i burned all the hairs off my arms many times due to glovebox fire.


--------------------
:hotidea::hotidea::hotidea:

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Offlineskaterickets
Musician
Male User Gallery


Registered: 06/26/08
Posts: 653
Last seen: 10 years, 6 months
Re: sterile glovebox [Re: Groomies]
    #8699848 - 07/29/08 10:25 PM (16 years, 5 months ago)

How is that possible?
Unless you keep the flame to sterilize inside the glove box?

:confused:

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InvisibleKrez
RamRod
Male User Gallery


Folding@home Statistics
Registered: 01/08/08
Posts: 1,504
Re: sterile glovebox [Re: skaterickets]
    #8699854 - 07/29/08 10:27 PM (16 years, 5 months ago)

Exactly!  People probably get all amped up and keep a lighter or alcohol lamp inside and forget bout spraying alcohol in the box and boom...Quick flash fire!


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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OfflineGroomies
Ghost
 User Gallery


Registered: 08/16/07
Posts: 1,119
Last seen: 8 years, 1 month
Re: sterile glovebox [Re: Wonderboyz]
    #8699860 - 07/29/08 10:28 PM (16 years, 5 months ago)

i use alcohol to clean my knife or needle.  then i light it with a lighter. then POOOFF!!!! fire!


--------------------
:hotidea::hotidea::hotidea:

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InvisibleKrez
RamRod
Male User Gallery


Folding@home Statistics
Registered: 01/08/08
Posts: 1,504
Re: sterile glovebox [Re: Groomies]
    #8699873 - 07/29/08 10:32 PM (16 years, 5 months ago)

I can just see it now...lol :hotidea:


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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