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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: inoculation question [Re: just me]
#8679199 - 07/25/08 04:04 AM (16 years, 5 months ago) |
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You can say fuck the prints and just isolate and make LC jars..
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
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Re: inoculation question [Re: Damion5050]
#8680710 - 07/25/08 02:58 PM (16 years, 5 months ago) |
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Quote:
Damion5050 said: You can say fuck the prints and just isolate and make LC jars..
WHAT!?! lol. now im just confused
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: inoculation question [Re: NiCKh609]
#8680895 - 07/25/08 04:02 PM (16 years, 5 months ago) |
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Quote:
NiCKh609 said:
Quote:
Damion5050 said: You can say fuck the prints and just isolate and make LC jars..
WHAT!?! lol. now im just confused
Well when u isolate.. It's basically cloning say you have and shroom that pinned really fast and it has good size... You can take a biopsy from that shroom and isolate the strain and grow that shroom that way your flushes are almost always the same...
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
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Re: inoculation question [Re: Damion5050]
#8680916 - 07/25/08 04:10 PM (16 years, 5 months ago) |
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Quote:
Damion5050 said:
Quote:
NiCKh609 said:
Quote:
Damion5050 said: You can say fuck the prints and just isolate and make LC jars..
WHAT!?! lol. now im just confused
Well when u isolate.. It's basically cloning say you have and shroom that pinned really fast and it has good size... You can take a biopsy from that shroom and isolate the strain and grow that shroom that way your flushes are almost always the same...
that sounds like something i wouldnt be able to do. It sounds like some straight up micologist scientific stuff lol
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 5 years, 6 months
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Re: inoculation question [Re: NiCKh609]
#8680972 - 07/25/08 04:23 PM (16 years, 5 months ago) |
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--------------------
 
--------------------------------------------------
-pEaCeLoVeGoDbLeSs-
"The Downfall of Mankind; is Believing He Has Limitations."
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: inoculation question [Re: just me]
#8681220 - 07/25/08 05:26 PM (16 years, 5 months ago) |
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Actually it is really easy as long as everything is sterile... I am on my first grow right now, I have not cloned yet but when my cakes start pinning if there is something that looks promising I may clone it..
But me being a noob I was able to make myself 1 quart of Golden Teacher LC.. And 1 quart of Koh Samui LC... It was very simple and all I did was turn my bathroom into a clean room I washed the sink top down with bleach/water mix and the used oust to sanitize the air then did my LC jars in there..
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
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Re: inoculation question [Re: Damion5050]
#8681426 - 07/25/08 06:23 PM (16 years, 5 months ago) |
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hmmmm. do u kno the link to a tek so i can see if it would be something i could handle?
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: inoculation question [Re: NiCKh609]
#8681817 - 07/25/08 08:49 PM (16 years, 5 months ago) |
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Quote:
NiCKh609 said: hmmmm. do u kno the link to a tek so i can see if it would be something i could handle?
Here is a LC TEK..
Also I'll explain what I did.. I was using a quart jar..
1. Take jar clean with bleach water then rinse really good.. 2. Boil water on stove for 5 minutes.. 3. Put three cups of boiling water in quart jar.. 4. Add 3 Tablespoons of Honey to the hot water in jar.. 5. Stir very well with sterile spoon.. 6. Drop in Marble.. This helps stir the Myco later.. 7. Put lid on jar, don't tighten all the way.. 8. Put 2 coffer filters over lid held down by rubber band.. 9. PC jar for 20 minutes at 15 p.s.i.. Let cool completely.. 10.Take cool jar out of PC and tighten the lid while filters are still on.. 11.Wipe lid down with rubbing alcohol.. 12.Flame needle with spore solution and also wipe down with rubbing alcohol.. 13.Inoculate jar with 1-2cc's.. 14.Put in dark place for 5-7 days..
And that all.. You put about 3-4% of honey by weight so.. If you have 3 cups of water that is roughly 600-700 ml's.. So 4% of 700 ml's is 28 ml's which is roughly 2.8 tablespoons.. So I always do 3 cups of water and 3 tablespoons of honey in a quart jar..
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
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Re: inoculation question [Re: Damion5050]
#8681915 - 07/25/08 09:14 PM (16 years, 5 months ago) |
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thanks bro =). i think ima just stick with the classic way though.
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
Edited by NiCKh609 (07/25/08 09:19 PM)
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: inoculation question [Re: NiCKh609]
#8683156 - 07/26/08 05:09 AM (16 years, 5 months ago) |
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Quote:
NiCKh609 said: thanks bro =). i think ima just stick with the classic way though.
Well the way I do it is very very simple.. The only difference between my way and the tek I linked you to is that I use boiling water because I boil off the contaminants and it helps melt the honey.. And I clean my jar with bleach water to help keep in sterile.. Then the use of honey instead of karo because honey is said to be more nutritious and the myco likes the honey better then the karo.. Those are the only differences between the linked tek and the way I do it.. I read all the LC teks then kinda mixed them together to make a nice sterile way of doing things.. That way I am not wasting my time or money on spore syringes..
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smokedout420
The EnlightenedOne



Registered: 12/16/07
Posts: 484
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Re: inoculation question [Re: NiCKh609]
#8683190 - 07/26/08 05:52 AM (16 years, 5 months ago) |
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cloning is simple just take your time and be sterile. make sure to pull the biggest one out the cluster and you'll fine if you dont have a cluster then the biggest shroom.
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
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Re: inoculation question [Re: Damion5050]
#8685398 - 07/26/08 07:21 PM (16 years, 5 months ago) |
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u said it takes like 2-3 days instead of like 2 weeks... but u had to wait a week with the jars in the dark anyways. so your going through all that work and risking contamination to save like 3 days?
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: inoculation question [Re: NiCKh609]
#8685991 - 07/26/08 09:37 PM (16 years, 5 months ago) |
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Quote:
NiCKh609 said: u said it takes like 2-3 days instead of like 2 weeks... but u had to wait a week with the jars in the dark anyways. so your going through all that work and risking contamination to save like 3 days?
But look at it this way u wait one week for the jar to mature then u have it u can store it in your frigerator for up to 6 months.. So the first time yes u have to wait for it to grow but after that u just take the myco out of the jar with a syringe and use it to inoculate grain jars or brf and it cuts the colonization time to 4-7 days instead or 2-3 weeks
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
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Re: inoculation question [Re: Damion5050]
#8689400 - 07/27/08 05:57 PM (16 years, 5 months ago) |
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im growin on the DL so i cnt keep it in the fridge without gettin asked a million questions tht i wont have answers to. if i did do it tht way could i just keep keep it in like a crawlspace or somewhere cool or does it have to b COLD?
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: inoculation question [Re: NiCKh609]
#8689410 - 07/27/08 06:01 PM (16 years, 5 months ago) |
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You don't have to keep it cold if you don't want to but if you were to store it in a cold place it would last longer..
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
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Re: inoculation question [Re: Damion5050]
#8689426 - 07/27/08 06:04 PM (16 years, 5 months ago) |
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nd i have to suck up the stuff on the top into the syringe? or the liquid. cus i read there will b a layer of myco on top?
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
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smokedout420
The EnlightenedOne



Registered: 12/16/07
Posts: 484
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Re: inoculation question [Re: NiCKh609]
#8689526 - 07/27/08 06:27 PM (16 years, 5 months ago) |
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NiCKh609
Warrior



Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
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hmmm. tht rlly just complicates things. i think i gotta get good with the pf tek b4 i try any variation or anything
-------------------- Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz
I have never grown or takin any illegal drugs.
Easiest Cloning Tek"
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