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Offlinedeathw
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first contam (pic)
    #8686766 - 07/27/08 12:57 AM (16 years, 5 months ago)

well I found my first contam yesterdday and moved the jar to a higher shelf just incase it wasnt contams but I looked again earlier today and it def was green so I wrapped the jar up and ditched the whole jar than about 12. Hours later I was looking at the other jars of its stain and boom another one now Im getting afraid I have 5 Australia jars about 90% done and about 15 other jars around the leftover GT that I havent seen contams yet should I be worried? Should I just move all the GT jars away from the others? The second jar of gt I saw contams I  wrapped in foil and put it on the higher shelf away from them Im gonna ditch it when I get home from work any advice on what to do to protect the others?


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Edited by deathw (07/27/08 03:38 AM)

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Offlinedeathw
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Re: first contam [Re: deathw]
    #8686771 - 07/27/08 12:58 AM (16 years, 5 months ago)

also the contam was VERY small I def caught it quick


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Re: first contam [Re: deathw]
    #8686934 - 07/27/08 02:00 AM (16 years, 5 months ago)

any advice on what I should do with the other jars?


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Re: first contam [Re: deathw]
    #8686975 - 07/27/08 02:15 AM (16 years, 5 months ago)

Get rid of all the the jars that are contaminated and clean the area where the contamination occurred. I used to try to save jars and keep them around, this obviously proved to be a bad idea, even if the jars are sealed and do not spew out contaminants it is still a breeding ground and an accident waiting to happen. Get rid of the them. If you are seeing multiple jars of the same strain develop contamination, it is usually a pattern and means that during your inoculation or sterilization procedure you may not have been as sterile as you think. Be careful and don't try to cut time by skipping sterilization techniques or time to build a glove box (if you haven't), preparation is key, otherwise you'll pay for it later. Many people say you can get away without a glove box (I was one of them) and you can. You can achieve great results, 80%+ success, but since I started using a glove box.... 100%  so do as you will, I wish you the best.

Peace,

I.T.


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Edited by newmodel (07/27/08 02:17 AM)

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Re: first contam [Re: newmodel]
    #8687096 - 07/27/08 03:00 AM (16 years, 5 months ago)

i am using a glovebox, i already ditched the one i know for sure that had the contams, and im gonna ditch the other one that im almost 99% sure has contams. now im just worried that the 5 jars of EQ i did with the GT is gonna be screwed.. Now what about where i keep the jars? should i clean up there? ill take a picture of where there being kept .. damn and i mean they were picking up speed since the 3rd day.. im more worried about those jars screwing with my 90% australia jars.. and 65% hawaiian jars.. from just being in the same area... glovebox and storage are in different areas  just so you know..


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Re: first contam [Re: deathw]
    #8687158 - 07/27/08 03:37 AM (16 years, 5 months ago)

also i am worried about this one australia jar, pic explains all.. is it piss?



ill upload a pic of the contam to confirm though.


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Re: first contam [Re: deathw]
    #8687171 - 07/27/08 03:55 AM (16 years, 5 months ago)

I could very well be wrong (with everything I say in life), but your jar looks like its sweating its ass off. I'd lower the temp, fan more, etc. My operating procedures with cakes are as follows:

1. Keep incubation at no more than 84 degrees environmentally for the jars

2. Rotate the jars, dependent on how their incubating. I generally have 12 at a time incubating, 6 on the bottom of the incubating chamber, 6 on top of those. I try to distribute the heat as well as possible - definitely air the chamber out once a day. The mycelium can't enjoy that rotten ass smell anymore than I can.

3. I give it half a week to a full week, and I take it out and birth. I've never had a fully colonized cake, and never had a problem with flushing some nice weight/fungus.

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Re: first contam [Re: c1dh3d]
    #8687182 - 07/27/08 04:09 AM (16 years, 5 months ago)

they are sweating pretty good, but the temp i keep them in all day is between 76-81 been getting hard since the heat waves and still gotta make a living, heh, also the humidity is around 60-63

i try and rotate them actually by switching them up but only parts that are grown have sweat anything that doesnt have myc doesnt have sweat

i also figured about a half a week to a full and i was going to bith it/them.

anybody else has any insight on this?


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Edited by deathw (07/27/08 04:23 AM)

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Re: first contam [Re: deathw]
    #8687411 - 07/27/08 07:27 AM (16 years, 5 months ago)

Should I be worried about the sweat?


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Re: first contam [Re: deathw]
    #8687545 - 07/27/08 08:43 AM (16 years, 5 months ago)

No, that's standard.  The moisture is part of the colonization process.  What you call green contams could just be bruising.  If it's a real mold, it will spread quickly--just separate any questionable jars from the rest of the grow.

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Re: first contam [Re: MLBjammer]
    #8687613 - 07/27/08 09:17 AM (16 years, 5 months ago)

I'm on my phone but I posted some pics in the contam forum on my Hawaiian jar and Australia jar that had me concerned but the mold did grow I had it stored at a friends house to see if it was or wasn't when I first saw it, just checked it and it grew but please check out my post in the contam section much appreciated


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Re: first contam [Re: deathw]
    #8689592 - 07/27/08 06:48 PM (16 years, 5 months ago)

The only times that I've seen my jars sweat, they stopped innoculating when there was hardly anything to fruit - a tiny spot of mycelium. Didn't turn colors, just stopped doing anything altogether. Though I have no real reason to believe sweating in the jars is a bad thing, I don't like when I see it - it's never meant any good news to me.

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Re: first contam [Re: c1dh3d]
    #8689615 - 07/27/08 06:56 PM (16 years, 5 months ago)

I'm still see growth in my Australia jars and my Hawaiian jars, Hawaiian slower than the aus by they were both injected at the same time and room temp is always standard 77-80? I'm going to try and keep the temp in the 76-77 range to maybe try and bring it down you guy are making me nervous, 1 aus jar is about a dime shape away from top to bottom covered in myc


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Edited by deathw (07/27/08 07:02 PM)

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Re: first contam [Re: deathw]
    #8689648 - 07/27/08 07:04 PM (16 years, 5 months ago)

Sorry, I didn't mean to scare ya :P It could and probably does mean absolutely nothing, I just noticed a pattern with my jars and seeing water droplets on the inside of it.

Standard incubation temp is right around 82-84 degrees, but room temperature will work fine - just makes it a whole heck of a lot slower. I'd definitely keep the temps in the higher 70 vs the lower 70's if you can.

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Re: first contam [Re: c1dh3d]
    #8689720 - 07/27/08 07:22 PM (16 years, 5 months ago)

Sweating should not be a problem, I have had many of my jars sweat and come out just fine, like others suggested watch the temps but don't lose sleep over it. The pic you posted, did you make an outline with blue marker on the jar? If so, then perhaps you have some bruising or a contamination, but if you just outlined something, then I see no reason to worry.


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Re: first contam [Re: newmodel]
    #8689928 - 07/27/08 08:15 PM (16 years, 5 months ago)

yeah I outlined with a Blue fine point sharpie so I can keep track of the one Australia jar and I outline the myc to make sure growth is still happening, but I only check them out like once a day but ever since the GT showed contams I'm a little more worried than normal about the others so I've been looking a little more often, 12 in the morning will make my aus and haw jars 2 weeks old and they've been sweating since the begining and like I said the temp never passes 80-81 so maybe I'm just bugging but I mean it is my first grow and until I have my own spores to inject as I please or experiment than I bet I'll stay worried, I bet if I look that aus jar its covered 100% from top to bottom now the inside I don't know how long do you normaly wait until a jar is covered from top to bottom until you dunk?


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Re: first contam [Re: deathw]
    #8690620 - 07/27/08 10:54 PM (16 years, 5 months ago)

I know a week after there fully covered is recomended but could one always do 5 days and than dunk?


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