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Offlineinsomniac20
4th Horseman
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Registered: 07/13/08
Posts: 315
Loc: Mid-West
Last seen: 15 years, 5 months
Cloning
    #8687318 - 07/27/08 06:01 AM (16 years, 5 months ago)

If I were to take a fresh shitake from work could I make a water culture out of a biopsy?

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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: Cloning [Re: insomniac20]
    #8687324 - 07/27/08 06:07 AM (16 years, 5 months ago)

Yes, providing it is fresh enough and clean enough. You'd be much better off clonging to agar though, to allow yourself the ability to isolate away from contaminants. Commercial mushrooms are far from clean.

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Offlineinsomniac20
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Re: Cloning [Re: wisp]
    #8687330 - 07/27/08 06:11 AM (16 years, 5 months ago)

Thanks That exactly what I wanted to know.

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Offlineinsomniac20
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Re: Cloning [Re: insomniac20]
    #8687333 - 07/27/08 06:12 AM (16 years, 5 months ago)

How fresh would it have to be? Day's wise? bad grammar

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InvisiblePhraggle
Psilocybe Therapy Advocate


Registered: 04/19/06
Posts: 161
Re: Cloning [Re: insomniac20]
    #8687335 - 07/27/08 06:12 AM (16 years, 5 months ago)


Edited by Phraggle (07/27/08 06:13 AM)

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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: Cloning [Re: insomniac20]
    #8687340 - 07/27/08 06:20 AM (16 years, 5 months ago)

Quote:

insomniac20 said:
How fresh would it have to be? Day's wise? bad grammar




Within 48 hours of picking is preferable. The more time is has been since harvest, the less viable it will be.

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Offlineinsomniac20
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Registered: 07/13/08
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Re: Cloning [Re: insomniac20]
    #8687342 - 07/27/08 06:22 AM (16 years, 5 months ago)

Cool, Thanks.

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