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OfflineDamion5050
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PC SYRINGES ?
    #8685997 - 07/26/08 09:38 PM (16 years, 5 months ago)

I was wondering I have an empty spore syringe that I want to use I am gona PC it to make it sterile..  When I PC it should it be filled with water or empty??

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InvisibleLedd
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Re: PC SYRINGES ? [Re: Damion5050]
    #8686070 - 07/26/08 09:50 PM (16 years, 5 months ago)

There is no use in PC'ing it empty.

Fill it with water and PC. Then the water will be sterile and ready for use.

or...

Don't PC. Boil water. Suck up water till full. Let sit two minutes. Discard down drain. Reapeat two more times. Final time, let liquid cool completely.

At this time, either method will have provided a final product of a clean syringe with sterile water.

Look up syringe teks for methods of introducing spores to the water.


:peace:Led


--------------------
We mean nothing we do not mean to do.

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OfflineBrandNewbie
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Re: PC SYRINGES ? [Re: Damion5050]
    #8686081 - 07/26/08 09:52 PM (16 years, 5 months ago)

Fill it with water, wrap it with tin-foil, leave the plastic cap off the needle, make sure the needle is pointing UPWARD. It helps to PC it with some jars. :rockon:


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Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.

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OfflineBrandNewbie
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Re: PC SYRINGES ? [Re: BrandNewbie]
    #8686086 - 07/26/08 09:53 PM (16 years, 5 months ago)

Ledbonzo86's method also works well!


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Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.

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OfflineJeets
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Re: PC SYRINGES ? [Re: BrandNewbie]
    #8686204 - 07/26/08 10:16 PM (16 years, 5 months ago)

wrap it with tinfoil bro : )

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Offlinejust me
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Re: PC SYRINGES ? [Re: Jeets]
    #8686265 - 07/26/08 10:28 PM (16 years, 5 months ago)

i dont wrap. should i? :lol:

just put them needle up in the PC filled with distilled water. like they said, then you have a nice sterile syringe.

you can take ur prints then and make a quick spore syringe.


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"The Downfall of Mankind; is Believing He Has Limitations."

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OfflineDamion5050
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Re: PC SYRINGES ? [Re: Ledd] * 1
    #8686962 - 07/27/08 02:09 AM (16 years, 5 months ago)

Well the reason I ask water or not is because I don't actually need the water I have a 1 quart jar of Golden Teacher LC

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OfflineNexusOne
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Re: PC SYRINGES ? [Re: Damion5050]
    #8687048 - 07/27/08 02:37 AM (16 years, 5 months ago)

Throw it in the pressure cooker and try to make sure it won't melt.  Pressure and heat will take care of the rest.

You do not need water in the syringe to sterilize it.

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Invisiblerugergirl79
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Re: PC SYRINGES ? [Re: NexusOne]
    #8687053 - 07/27/08 02:42 AM (16 years, 5 months ago)

Quote:

NexusOne said:


You do not need water in the syringe to sterilize it.


<<<< why not kill two birds with one stone....fill the syringe and then pc it.  you will not then have to make extra steps to make sure your water is sterile......:smirk:

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OfflineNexusOne
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Re: PC SYRINGES ? [Re: rugergirl79]
    #8687069 - 07/27/08 02:49 AM (16 years, 5 months ago)

Read his post.  He plans to use the syringe to inject a liquid culture that he already has, so he doesn't need any sterilized water.

His question was phrased clearly and concisely and the answer should have been simple, too.  One way or the other, it makes no difference to the sterility of the syringe whether there is water in it or not.

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Invisiblerugergirl79
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Re: PC SYRINGES ? [Re: NexusOne]
    #8687095 - 07/27/08 02:59 AM (16 years, 5 months ago)

Quote:

NexusOne said:
Read his post.  He plans to use the syringe to inject a liquid culture that he already has, so he doesn't need any sterilized water.

His question was phrased clearly and concisely and the answer should have been simple, too.  One way or the other, it makes no difference to the sterility of the syringe whether there is water in it or not.


  <<You dont have to be so.....:crankey:, i went on the original post........he or she didnt specify whether it was for a spore solution for inoculation or a LC......so i guess with that, it wasnt  as you say "clear and concise!:smirk:  Original post....
Quote:

Damion5050 said:
I was wondering I have an empty spore syringe that I want to use I am gona PC it to make it sterile..  When I PC it should it be filled with water or empty??





Have a wonderful morning.  kat:thumbup:

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OfflineNexusOne
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Re: PC SYRINGES ? [Re: rugergirl79]
    #8687123 - 07/27/08 03:16 AM (16 years, 5 months ago)

Quote:

rugergirl79 said:You dont have to be so.....:crankey:, i went on the original post........he or she didnt specify whether it was for a spore solution for inoculation or a LC......so i guess with that, it wasnt  as you say "clear and concise!:smirk:




I didn't offer any insult but you're free to take one wherever you please.  I answered a question and you responded (to my post and not the original one) without reading the post immediately prior to my own.  I simply pointed that out and you can apply whatever tone to it that you wish.

The question was, in fact, very clear and concise.  He didn't ask about killing birds or how many stones he should use to do it, he asked whether or not it was necessary to put water in a syringe to sterilize it in a pressure cooker.  That question has been answered, so let's let this thread drop.

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InvisibleVV Cephei
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Re: PC SYRINGES ? [Re: NexusOne]
    #8687187 - 07/27/08 04:11 AM (16 years, 5 months ago)

Here's a post by RR that I found in this thread that gives another take on whether to PC syringes:

Quote:

I've posted this for years, but there's absolutely NO reason to pc syringes. All you need to do is boil in water for an hour or so. In fact, I boil them right up until it's time to take them out and use.
RR




If you're going to PC some jars anyways, then sure, throw in a few syringes at the same time. But I thought it was worth mentioning that if all that you wanted to do was sterilize a syringe, then using the PC wasn't necessary.

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OfflineDamion5050
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Re: PC SYRINGES ? [Re: VV Cephei]
    #8687308 - 07/27/08 05:52 AM (16 years, 5 months ago)

Well thank you everyone for the answers.. Sorry to cause a small scuffle out of it lol...  Thx for the answers.. :shroomer: :shroomer::shroomer::shroomer:

Edited by Damion5050 (07/27/08 06:56 AM)

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Offlinejust me
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Re: PC SYRINGES ? [Re: Damion5050]
    #8688053 - 07/27/08 11:50 AM (16 years, 5 months ago)

theyre husband and wife, they do this all the time

:nonono:

but THIS is for everyone

:hug:


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"The Downfall of Mankind; is Believing He Has Limitations."

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Re: PC SYRINGES ? [Re: just me]
    #8688200 - 07/27/08 12:42 PM (16 years, 5 months ago)

I would recommend as the above did. Wrap in foil.

Or seal in a bag before PC'ing

This avoids any contams when taking it out of the pc and transferring to your "Sterile Environment"

Good Luck


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Someone actually said: "some 1 told me to try long brown rice and water and vermiculite and pressure cook for 1 hour i did but nothing happen that is y was wonting to no if i need spores ""

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Re: PC SYRINGES ? [Re: psylopsycho]
    #8688249 - 07/27/08 01:00 PM (16 years, 5 months ago)

just suck in boiling water, empty the syringe and fill it up again, do it for like 2-3 times and everything in the syringe will be killed.


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Invisiblerugergirl79
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Re: PC SYRINGES ? [Re: just me]
    #8688526 - 07/27/08 02:23 PM (16 years, 5 months ago)

lol  im extra :crankey: this week...lol  :angrykidface::mad2::mad:  I will get over it.  kat

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OfflineDamion5050
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Re: PC SYRINGES ? [Re: Bridgeburner]
    #8688531 - 07/27/08 02:25 PM (16 years, 5 months ago)

Sweet I'll just do what RR suggested, boiling in water cause of course everyone knows he is the man  :bow:

Another question if people are still looking I am thinking about doing some mono tubs too but.. I am not sure how to light them.. I have some 6500 kelvin compact fluerscents..  But I am not sure how to get the light into the mono tub, I was thinking about like mounting the light some how into the tub but then there is an electrical hazard ??

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Invisiblerugergirl79
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Re: PC SYRINGES ? [Re: Damion5050]
    #8688551 - 07/27/08 02:28 PM (16 years, 5 months ago)

you are going to put water in your syringes....hummm what a novel idea!!!!!  Dont let NexusOne hear ya....kat

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