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Offlinejust me
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odd spore solution in my syringe
    #8680941 - 07/25/08 04:16 PM (16 years, 5 months ago)

i made up some new syringes to make up some new LC's to make up some new tester cakes...if youre with me on this youre with me.

i did the sterilize a jar,scrape some print. squirt in sterile water, extract SS tek.

so the jars i had were old jars. they had contamed cakes in them. i cleaned them in the sink with soap and water, and then PC'd them for over an hour.

the syringes, also reused, and filled with distilled water were also in the PC for this time.

so today after allowing to cool overnight, i scraped the spores, and sucked em into the syringe.

upon injecting them into my LC's, i noticed both syringes of one particular strain thats been givin me a lot of grief had two patches in it that looked like mycelium.

how can this be if theyre only hours old???



my guess is they came from the existing SS that was in the syringe. but the syringes were drawn and shot out with boiling water several times b4 i stored them. then even after PC'ing they turned out with myc like substance in them :shrug:


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

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OfflineBrandNewbie
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Re: odd spore solution in my syringe [Re: just me]
    #8680981 - 07/25/08 04:24 PM (16 years, 5 months ago)

Hey you. You might have to isolate with agar. Without pics no one can tell. If I use jars that I think had contaminates in them, I soak them in a bleach solution, then dishwasher, then PC. And DO NOT use the lids again! They are cheap, why risk it?  Gotta go, be on later...


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Offlinejust me
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Re: odd spore solution in my syringe [Re: BrandNewbie]
    #8680999 - 07/25/08 04:28 PM (16 years, 5 months ago)

i didnt reuse the lids.

and this is only bcuz i bought the wrong tape, and it was impossible to get off the fuckin lid :lol:


you dont need a picture, picture it with your mind.

a freshly made syringe with a chunk of what appears to be mycelium floating in it :crazy2:

only thing that sux is now i gotta wait for the LC, and if it doesnt SHOW me theres a contam i gotta test it out, and wait for that.


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

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Invisiblethemange
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Re: odd spore solution in my syringe [Re: just me]
    #8681033 - 07/25/08 04:36 PM (16 years, 5 months ago)

is it paper maybe from where you scraped the spores off?

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Offlinejust me
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Re: odd spore solution in my syringe [Re: themange]
    #8681057 - 07/25/08 04:41 PM (16 years, 5 months ago)

they were on foil.

the only thing i could think other thn myc. is maybe SOMEHOW some of the paper towell i was using((soaked in 70%iso) to cover the hole i put in the foil covering the opening of the jar to scrape the spore into(got all that:confused: )let a piece fall into the jar.

the only thing strange about THAT scenario is that i made 3 syringes one of a strain that DOESNT have anything like this in it, and 2 of a strain that DOES have this in each syringe.


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

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Invisiblethemange
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Re: odd spore solution in my syringe [Re: just me]
    #8681069 - 07/25/08 04:44 PM (16 years, 5 months ago)

no way it could be myc. i think your guess is probably the best bet.

pretty crazy tho.

its probably not sterile anywho if you have foreign matter in your syringe.

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Offlinejust me
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Re: odd spore solution in my syringe [Re: themange]
    #8681184 - 07/25/08 05:16 PM (16 years, 5 months ago)

yeah thats what i was thinkin.

what if it is leftover from a solution from b4, and by PCing the water in the syringe it hydrated WHATEVER it was.

wouldnt it be sterile due to the PCing?


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

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Invisiblethemange
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Re: odd spore solution in my syringe [Re: just me]
    #8681201 - 07/25/08 05:21 PM (16 years, 5 months ago)

i would say so.

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