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OfflineNiCKh609
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inoculation question
    #8678660 - 07/25/08 12:09 AM (16 years, 5 months ago)

hmmm. Well all i hear about is sterility... well, instead of making a whole sterilization chamber for inoculating my jars, couldnt i just put one of those lysol whipes over the jars, rub my hands w/ rubbing alcohol, sterlize the syringe, then just inoculate through the lysol wipe nd micropore tape instead of making a while entire chamber to inoculate in?

Has anyone ever tried this or would i be taking a big risk doing this? I just thought that since they kill 99.99% of germs thats a WHOLE lot cleaner then a big plastic box rubbed down with alcohol with 2 big holes cut in it.

Any feedback would be greatly appreciated :grin:


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: NiCKh609]
    #8678671 - 07/25/08 12:12 AM (16 years, 5 months ago)

I never used a steriliztion chamber...Just oust the area turn off the a/c and any fans.  Flame the needle and throw some micropore tape on it and youll be fine!


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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Re: inoculation question [Re: NiCKh609]
    #8678682 - 07/25/08 12:13 AM (16 years, 5 months ago)

i am a noob my self but i dont see why it would not work  just make sure u spray the SHIT out of the room u are working in first


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineMarioTrip
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Re: inoculation question [Re: Krez]
    #8678686 - 07/25/08 12:14 AM (16 years, 5 months ago)

Pretty much though gloveboxes do come in handy for certain types of work!


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OfflineMHbound
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Re: inoculation question [Re: Krez]
    #8678687 - 07/25/08 12:14 AM (16 years, 5 months ago)

I go into a small bathroom...Spray the shit out of it with lysol. Then go outside for 5-10 minutes and go back in. Keep my hands clean and everything else. Flame the needle inoculate one jar, flame again inoculate another jar, and so forth. Flame the needle and put the cap back on...


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OfflineNiCKh609
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Re: inoculation question [Re: MHbound]
    #8678705 - 07/25/08 12:17 AM (16 years, 5 months ago)

yea cus in the lets grow mushrooms vid he uses a chamber for it i was like "thats retarded. cnt u just clean up a small space, put a lysol whip over the inoculation points nd tht will keep our any bad stuff."


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: MHbound]
    #8678711 - 07/25/08 12:18 AM (16 years, 5 months ago)

Quote:

MHbound said:
I go into a small bathroom...Spray the shit out of it with lysol. Then go outside for 5-10 minutes and go back in. Keep my hands clean and everything else. Flame the needle inoculate one jar, flame again inoculate another jar, and so forth. Flame the needle and put the cap back on...




When he says spray the shit out of it he aint kiddin!:grin:


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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OfflineNiCKh609
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Re: inoculation question [Re: Krez]
    #8678722 - 07/25/08 12:21 AM (16 years, 5 months ago)

how long should i heat the needle for with a lighter? like how long until its red hot?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: NiCKh609]
    #8678725 - 07/25/08 12:22 AM (16 years, 5 months ago)

Quote:

NiCKh609 said:
how long should i heat the needle for with a lighter? like how long until its red hot?




you got it


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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Re: inoculation question [Re: Krez]
    #8678730 - 07/25/08 12:23 AM (16 years, 5 months ago)

i have a sweet glove box i will post pics of it


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Re: inoculation question [Re: noobieshroomie]
    #8678741 - 07/25/08 12:26 AM (16 years, 5 months ago)

u will see the tip GLOW red thats good


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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OfflineNiCKh609
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Re: inoculation question [Re: noobieshroomie]
    #8678745 - 07/25/08 12:26 AM (16 years, 5 months ago)

and also, when would i pic them? b4 they re;ease thier spores or after: (ima b making prints. ill have a bunch of pics of my setup and everything in my journal sometime this month)


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: NiCKh609]
    #8678756 - 07/25/08 12:29 AM (16 years, 5 months ago)

well if your makin prints youll wanna do it before they drop em


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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OfflineNiCKh609
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Re: inoculation question [Re: Krez]
    #8678774 - 07/25/08 12:32 AM (16 years, 5 months ago)

Quote:

Krez said:
well if your makin prints youll wanna do it before they drop em




i know when u start to see some purple spores u take the caps nd foil nd let em sit.  iread up on tht. i mean like when should i pick them to get the best high from them... and ill also b makin prints lol. AND after u make spore prints can you still eat the caps to trip or no?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: NiCKh609]
    #8678786 - 07/25/08 12:34 AM (16 years, 5 months ago)

yea you can still eat em after you print them...i normally pick em when the veil tears.


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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OfflineNiCKh609
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Re: inoculation question [Re: Krez]
    #8678796 - 07/25/08 12:38 AM (16 years, 5 months ago)

so viel tears, pick to dry nd eat

i let some grow until i see a few spores start droppin then make prints?

and if i print them on aluminium foil after theyve left there print can i like close the foil up? like for it in half nd crimp the edges, then keep it in a cool place so i can make sure no light gets in? or do i have to leave the foil flat?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: NiCKh609]
    #8678823 - 07/25/08 12:42 AM (16 years, 5 months ago)

you can close the foil up...Make sure your as sterile as possible.  Use a glovebox if you have one.  You want the cap as flat as possible.  Here ya go this should help http://www.shroomery.org/8404/Spore-printing-Pictorial


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)

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OfflineNiCKh609
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Re: inoculation question [Re: Krez]
    #8678853 - 07/25/08 12:52 AM (16 years, 5 months ago)

u gotta dry the spore pronts like u dry the shrooms?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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OfflineMHbound
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Re: inoculation question [Re: NiCKh609]
    #8679146 - 07/25/08 03:20 AM (16 years, 5 months ago)

Pick them before the veil breaks even if you don't plan to print them. Once they drop spores on your cakes/casings they cause the growth to slow, or come to a complete stop in those areas. On the 3rd/4th flushes you can let them open completely. They will be bigger by then and you will only get a 1-3, and probably won't see any other flushes anyway.


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Re: inoculation question [Re: NiCKh609]
    #8679154 - 07/25/08 03:23 AM (16 years, 5 months ago)

just thought id add that i asked this same question about a year ago :lol:

only it was thru an alcohol swab


--------------------

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OfflineDamion5050
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Re: inoculation question [Re: just me]
    #8679199 - 07/25/08 04:04 AM (16 years, 5 months ago)

You can say fuck the prints and just isolate and make LC jars..

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OfflineNiCKh609
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Re: inoculation question [Re: Damion5050]
    #8680710 - 07/25/08 02:58 PM (16 years, 5 months ago)

Quote:

Damion5050 said:
You can say fuck the prints and just isolate and make LC jars..




WHAT!?! lol. now im just confused


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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OfflineDamion5050
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Re: inoculation question [Re: NiCKh609]
    #8680895 - 07/25/08 04:02 PM (16 years, 5 months ago)

Quote:

NiCKh609 said:
Quote:

Damion5050 said:
You can say fuck the prints and just isolate and make LC jars..




WHAT!?! lol. now im just confused




Well when u isolate..  It's basically cloning say you have and shroom that pinned really fast and it has good size...  You can take a biopsy from that shroom and isolate the strain and grow that shroom that way your flushes are almost always the same...

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OfflineNiCKh609
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Re: inoculation question [Re: Damion5050]
    #8680916 - 07/25/08 04:10 PM (16 years, 5 months ago)

Quote:

Damion5050 said:
Quote:

NiCKh609 said:
Quote:

Damion5050 said:
You can say fuck the prints and just isolate and make LC jars..




WHAT!?! lol. now im just confused




Well when u isolate..  It's basically cloning say you have and shroom that pinned really fast and it has good size...  You can take a biopsy from that shroom and isolate the strain and grow that shroom that way your flushes are almost always the same...




that sounds like something i wouldnt be able to do. It sounds like some straight up micologist scientific stuff lol


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: NiCKh609]
    #8680972 - 07/25/08 04:23 PM (16 years, 5 months ago)

:hi5:


--------------------

--------------------------------------------------

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"The Downfall of Mankind; is Believing He Has Limitations."

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Re: inoculation question [Re: just me]
    #8681220 - 07/25/08 05:26 PM (16 years, 5 months ago)

Actually it is really easy as long as everything is sterile...  I am on my first grow right now, I have not cloned yet but when my cakes start pinning if there is something that looks promising I may clone it..

But me being a noob I was able to make myself 1 quart of Golden Teacher LC..  And 1 quart of Koh Samui LC...  It was very simple and all I did was turn my bathroom into a clean room I washed the sink top down with bleach/water mix and the used oust to sanitize the air then did my LC jars in there..

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OfflineNiCKh609
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Re: inoculation question [Re: Damion5050]
    #8681426 - 07/25/08 06:23 PM (16 years, 5 months ago)

hmmmm. do u kno the link to a tek so i can see if it would be something i could handle?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

Extras: Filter Print Post Top
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Re: inoculation question [Re: NiCKh609]
    #8681817 - 07/25/08 08:49 PM (16 years, 5 months ago)

Quote:

NiCKh609 said:
hmmmm. do u kno the link to a tek so i can see if it would be something i could handle?




Here is a LC TEK..

Also I'll explain what I did.. I was using a quart jar..

1. Take jar clean with bleach water then rinse really good..
2. Boil water on stove for 5 minutes..
3. Put three cups of boiling water in quart jar..
4. Add 3 Tablespoons of Honey to the hot water in jar..
5. Stir very well with sterile spoon..
6. Drop in Marble.. This helps stir the Myco later..
7. Put lid on jar, don't tighten all the way..
8. Put 2 coffer filters over lid held down by rubber band..
9. PC jar for 20 minutes at 15 p.s.i.. Let cool completely..
10.Take cool jar out of PC and tighten the lid while filters are still on..
11.Wipe lid down with rubbing alcohol..
12.Flame needle with spore solution and also wipe down with rubbing alcohol..
13.Inoculate jar with 1-2cc's..
14.Put in dark place for 5-7 days..

And that all..  You put about 3-4% of honey by weight so..  If you have 3 cups of water that is roughly 600-700 ml's.. So 4% of 700 ml's is 28 ml's which is roughly 2.8 tablespoons..  So I always do 3 cups of water and 3 tablespoons of honey in a quart jar..

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OfflineNiCKh609
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Re: inoculation question [Re: Damion5050]
    #8681915 - 07/25/08 09:14 PM (16 years, 5 months ago)

thanks bro =). i think ima just stick with the classic way though.


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

Edited by NiCKh609 (07/25/08 09:19 PM)

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Re: inoculation question [Re: NiCKh609]
    #8683156 - 07/26/08 05:09 AM (16 years, 5 months ago)

Quote:

NiCKh609 said:
thanks bro =). i think ima just stick with the classic way though. 


Well the way I do it is very very simple.. The only difference between my way and the tek I linked you to is that I use boiling water because I boil off the contaminants and it helps melt the honey.. And I clean my jar with bleach water to help keep in sterile..  Then the use of honey instead of karo because honey is said to be more nutritious and the myco likes the honey better then the karo..  Those are the only differences between the linked tek and the way I do it.. I read all the LC teks then kinda mixed them together to make a nice sterile way of doing things..  That way I am not wasting my time or money on spore syringes..

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Re: inoculation question [Re: NiCKh609]
    #8683190 - 07/26/08 05:52 AM (16 years, 5 months ago)

cloning is simple just take your time and be sterile.  make sure to pull the biggest one out the cluster and you'll fine if you dont have a cluster then the biggest shroom.

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Re: inoculation question [Re: Damion5050]
    #8685398 - 07/26/08 07:21 PM (16 years, 5 months ago)

u said it takes like 2-3 days instead of like 2 weeks... but u had to wait a week with the jars in the dark anyways. so your going through all that work and risking contamination to save like 3 days?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

Extras: Filter Print Post Top
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Re: inoculation question [Re: NiCKh609]
    #8685991 - 07/26/08 09:37 PM (16 years, 5 months ago)

Quote:

NiCKh609 said:
u said it takes like 2-3 days instead of like 2 weeks... but u had to wait a week with the jars in the dark anyways. so your going through all that work and risking contamination to save like 3 days?


But look at it this way u wait one week for the jar to mature then u have it u can store it in your frigerator for up to 6 months..  So the first time yes u have to wait for it to grow but after that u just take the myco out of the jar with a syringe and use it to inoculate grain jars or brf and it cuts the colonization time to 4-7 days instead or 2-3 weeks

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Re: inoculation question [Re: Damion5050]
    #8689400 - 07/27/08 05:57 PM (16 years, 5 months ago)

im growin on the DL so i cnt keep it in the fridge without gettin asked a million questions tht i wont have answers to. if i did do it  tht way could i just keep keep it in like a crawlspace or somewhere cool or does it have to b COLD?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Re: inoculation question [Re: NiCKh609]
    #8689410 - 07/27/08 06:01 PM (16 years, 5 months ago)

You don't have to keep it cold if you don't want to but if you were to store it in a cold place it would last longer..

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Re: inoculation question [Re: Damion5050]
    #8689426 - 07/27/08 06:04 PM (16 years, 5 months ago)

nd i have to suck up the stuff on the top into the syringe? or the liquid. cus i read there will b a layer of myco on top?


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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Invisiblesmokedout420
The EnlightenedOne
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Registered: 12/16/07
Posts: 484
Re: inoculation question [Re: NiCKh609]
    #8689526 - 07/27/08 06:27 PM (16 years, 5 months ago)

must say this is my favorite teks of all. remember practice makes perfect.

9er cloning tek

9er Tek Details: A collection of additions

TV Guide to Colonizing a Quart Jar in Three Days

grain lc

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OfflineNiCKh609
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Registered: 07/22/08
Posts: 317
Loc: America
Last seen: 15 years, 5 months
Re: inoculation question [Re: smokedout420]
    #8699446 - 07/29/08 08:58 PM (16 years, 5 months ago)

hmmm. tht rlly just complicates things. i think i gotta get good with the pf tek b4 i try any variation or anything


--------------------
Combustion?... is that the ability to cum on spencers mothers bust?" - Kenny Hotz

I have never grown or takin any illegal drugs.

Easiest Cloning Tek"

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