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ephman
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Registered: 01/29/05
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Last seen: 16 years, 4 months
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inoculation question... mycobag.
#8677911 - 07/24/08 09:03 PM (16 years, 5 months ago) |
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hi,
i injected the spores right into the middle of the bag, and then about 20 minutes later i mashed up the bag real good... real good. but i read that it is not good to inoculate right in the middle of bag. i did this last sunday, and nothing has started to show yet, which was fine i could wait. but today i read about the %22inject into the side%22 thing, so i did that also. basically in a 2 pound bag i put in about 6ccs on 2 different times. could i have really messed things up?
thanks,
ephman
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jeetered
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Registered: 07/07/06
Posts: 3,055
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Re: inoculation question... mycobag. [Re: ephman]
#8677955 - 07/24/08 09:10 PM (16 years, 5 months ago) |
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.... well, using spore solution for grain bags really isn't idea, ideally you would use liquid tissue culture, or grain to grain transfer.
it will take close to a week to see any signs of growth, probably longer seeing as you "shook" the bag up.
it's not advised to "shake" grains after you inoculate with multispore, you should let it sit and colonize to about 30%, the stir it up so to speak.
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MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
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Re: inoculation question... mycobag. [Re: jeetered]
#8678073 - 07/24/08 09:39 PM (16 years, 5 months ago) |
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Quote:
.... well, using spore solution for grain bags really isn't idea, ideally you would use liquid tissue culture, or grain to grain transfer.
it will take close to a week to see any signs of growth, probably longer seeing as you "shook" the bag up.
it's not advised to "shake" grains after you inoculate with multispore, you should let it sit and colonize to about 30%, the stir it up so to speak.
Also, inoculating in the middle of the bah wouldn't matter. The reason people say it is so that you can see the growth progress from the outside in...But if you do it in the middle no harm done. It could be colonizing in the middle, and you don't even know it yet. Don't shake them up either.
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CaptTrip
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Registered: 01/17/07
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Re: inoculation question... mycobag. [Re: MHbound]
#8678096 - 07/24/08 09:45 PM (16 years, 5 months ago) |
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It is a terrible idea to do a second injection of anything (spores, LC, more colonized grain), you are just adding a second opportunity to introduce contamination.
As far as injecting into the center of the bag, that has no effect on colonization. Like stated before, you do an injection onto the edge of the mycobag so you can see growth (or contamination at the injection point) as soon as possible. Just two days ago I injected 6cc's into the center of a 3 lb. rye bag. No harm done, just have to play the waiting game.
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Cambodian Grow Log
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nugjug
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Registered: 11/07/05
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Re: inoculation question... mycobag. [Re: ephman]
#8678098 - 07/24/08 09:46 PM (16 years, 5 months ago) |
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Just to expand on what jeetered already said. The spores have to pair up. If you shake them up they have a hard time finding a mate. By not using LC you have increased your colonization times which puts you in danger of getting a bacterial infection or some other kind of infection. I am crossing my fingers for you though.
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jeetered
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Registered: 07/07/06
Posts: 3,055
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Re: inoculation question... mycobag. [Re: nugjug]
#8678117 - 07/24/08 09:51 PM (16 years, 5 months ago) |
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Quote:
nugjug said: Just to expand on what jeetered already said. The spores have to pair up. If you shake them up they have a hard time finding a mate. By not using LC you have increased your colonization times which puts you in danger of getting a bacterial infection or some other kind of infection. I am crossing my fingers for you though.
yeah i didn't want to really expound on why, but,
when you shake up grains after inoculating with multispore, you'll more often then not end up with haploid tomentose monokaryotic mycelium, which is not rhizo, and either will not pin, or will pin and won't mature, or just a sparse shitty pinset,
if you let sit without shaking , you end up with diploid, diakaryotic rhizomorphic mycelium, which pins prolifically, and fruits more/longer
tomentose mycelium is the white cottony fluffy mycelium, while obviously rhizomorphic is ropey.
now, of course with grains, even with multispore, after about 30% colonization, feel free to shake the grains, as the myc is rhizo and diploid at this point, and will continue to be so after it repairs and expands.
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neek
Stranger

Registered: 07/19/08
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Last seen: 16 years, 5 months
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Re: inoculation question... mycobag. [Re: jeetered]
#8678434 - 07/24/08 11:21 PM (16 years, 5 months ago) |
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so shaking the bag fucks it up? great i thought it would help since everyone else said that on here
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 4,797
Loc: Let Me See Potato Salad!!...
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Re: inoculation question... mycobag. [Re: neek]
#8678444 - 07/24/08 11:23 PM (16 years, 5 months ago) |
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 Noobs. Crumbling after 20-30% colonization is fine... Otherwise Jeeterd covered all the Specifics already...
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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