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Offlinetrelli
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Registered: 06/29/08
Posts: 12
Last seen: 16 years, 4 months
5 days now and no pinning? What's wrong?
    #8675015 - 07/24/08 02:36 AM (16 years, 5 months ago)

This is my first time growing mushrooms and I started out with cakes and rye grains for casings.  I built an incubator out of an old refridgerator and use a small space heater to keep temp around 80 during colonization.  My cakes are slow to colonize because i used 1 part water, 1 part BRF, and 3 parts verm.(This was a tek I saw in a video on you tube) but I now know that it should be more like 1-1-2 instead of the 1-1-3 like I used.  The rye berries turned out great from colonization.  I took the colonized rye out and spread it evenly and gently into two aluminum meatloaf pans about 2%22 thick and then placed a freshly pcd casing layer of 50-50 approximately 1/2%22 thick and put foil over it with holes in the top and placed it back into the incubator(without looking) for 3 days until I saw some myc poking through.  After the 3 days I pulled it out of the incubator and put another 1/2 inch layer of casing mix on top of that.  I misted just enough to see the glisten of water.  I then put it into the FC with a timer set for 12 on and 12 off.  In two days I could see the myc coming through this second layer.  I continue to mist lightly once in the morning and once  in the evening.  My concern is that my incubator has a humidity of only about 30%25....did my casing dry out too much?
Am I not misting enough?  How long should it take to see some kind of growth?  My cakes are still in colonization phase after 4 weeks and have yet to be birthed.  The cakes are most likely to dry because of faulty cake tek as well as using the space heater to keep it 80 degrees in incubator.  Any thoughts from any pros out there would be very helpful.

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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: 5 days now and no pinning? What's wrong? [Re: trelli]
    #8675442 - 07/24/08 07:41 AM (16 years, 5 months ago)

it can take up to two weeks to start seeing pins.  it usually does for me.

no need to build an incubator the cakes and the rye will colonize just fine at room temp unless you live in an igloo.  they're a waste of time imo.

yea more than likely your cakes arent going to fair to well with that ratio.  2 cps brf, 2 cps water and 4 cps verm gives you about 10 cakes with the perfect water ratio.

that means you have 1" of casing on top of 2" of substrate. that's a little thick of a casing layer for such a shallow substrate depth.

what are you using to measure your humidity?  if that is an accurate reading then your humidity is way to low and you'll never get anything to pin. 

what are you using for a fruiting chamber and what are you doing to keep your humidity up besides misting?

you want your humidity to be as close to 100% as possible

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Offlinebeengonetoolong
Gravy Train Conductor
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Registered: 06/01/08
Posts: 523
Last seen: 14 years, 7 months
Re: 5 days now and no pinning? What's wrong? [Re: blood4blood]
    #8675460 - 07/24/08 07:48 AM (16 years, 5 months ago)

For some reason I always see pins on day 9.  Exactly.  Not sure wtf :smile:  Atleast it makes harvesting predictible for me.  I wouldnt be concerned until the 2-3 week mark.


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OfflinePowerOfTheCoir
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Registered: 07/02/08
Posts: 421
Last seen: 14 years, 24 days
Re: 5 days now and no pinning? What's wrong? [Re: beengonetoolong]
    #8676940 - 07/24/08 04:01 PM (16 years, 5 months ago)

You said that your incubator had an RH of 30%. This might not be a problem since the casing layer had a layer of foil over it when it was incubating. As long as you didn;t go nuts with the holes in the foil, there was plenty of humidity kept near the casing/sub). What's the RH in your FC?

Grains used alone (not spawned to bulk or even field capacity verm) will already have a problem with getting enough moisture to produce decent fruits. They'll be drinking heavily from the casing layer, so you have more of a need for a good RH than most people. You also need to keep misting frequently as long as you don't saturate the casing material. In the future, spawn the grains to something else. Hydrating, pasteurizing, and colonizing a bit of coir is EASY and even at a 1:1 ratio (barely even a bulk grow) the water reservoir provided by the coir will help immensely.

You didn't give any description of your FC aside from the 12/12 cycle (I'm guessing that's lighting). What are you using? A perlite tub like the Shotgun FC (use the search function for details) will work fine for cakes or cased subs.

A week isn't long anyway - even if everything is OK, you could have no pinning yet.

The two biggest problems people have that inhibit pinning are 1) Lack of near 100% RH in the pinning zone and 2) Lack of sufficient FAE.

Humidity - Since you are casing with 50/50, you will have a nice moist microclimate where the pins will form unless your RH in the FC is fairly low. Cased substrates can succeed in RH's that are a little too low for uncased subs. Still, anything you can do to get the RH in the FC up as close to 100% as possible will help. Casings can handle lower RH's, but they thrive at 100%.

FAE (Fresh Air Exchange)- If the substrate isn't getting enough O2 and having CO2 removed, it will assume it is still inside of a pile of cow dung. It won't form a mushroom until it reaches the surface. This can make it colonize forever. FAE and humidity are way more important in initiating pinning than light. Check your FC design and make sure that you have good FAE. Again, search for the Shotgun FC. It has literally hundreds of holes - that's the type of FAE that mushrooms need to thrive.  A few fannings with the lid throughout the day are a good addition to sufficient holes, but you need enough holes to start with. You'll have to fan every hour to make up for lousy ventilation.


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Shroom capsules with the Cap-M-Quik (pics)

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