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OfflineNiko
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Registered: 06/26/08
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P. azurescens mycelium only blueing if dicaryotic?
    #8659772 - 07/20/08 04:54 PM (16 years, 5 months ago)

Hello!

Now that I managed to seperate my P. azurescens mycelium from conatmination with help from this forum I have made some interesting discovery (at least to me!?)

Previously I suspected the fluffy whiteish growth to be some sort of contamination since it showed absolutely no rhizomorphic structures.
However I learned that P. azurescens often lacks these and successfully inoculated some jars with woodchips which are being colonized - but fairly slow.

Yesterday I noticed that one of the petridishes I inoculated with spores almost 2 months ago and that previously showed no sign of germination had since the last time I examined it produced some sort of slightly "rhizomorphic" growth.




I excised the center and transferred it to another dish, and several minutes later it started to turn blue!




None of the fluffy mycelium has ever shown any blueing, it stays white no matter how much you torture it - even the more mature one colonizing the woodchips.




My question:
Can only rhizomorphic growth exhibit blueing - maybe since it is dicaryotic?


Regards,
Niko

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OfflineNiko
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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: Niko]
    #8673329 - 07/23/08 07:23 PM (16 years, 5 months ago)

No comments!?

The excised agar piece has now been overgrown by more "rhizomorphic" mycelium.
It really grows much faster than the previous fluffy one which almost took 1 month to colonize a petri dish!




I also noticed some rhzomorphic growth in one jar containing fresh cut oak branches but it is not blueing.




Anyway - do you think it is better to select only the best growing mycelium or mix it all together in the end for an outdoor patch?


Regards,
Niko

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Invisiblejeetered
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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: Niko]
    #8673352 - 07/23/08 07:27 PM (16 years, 5 months ago)

selection by far is the best way to go, considering, that's the point in isolations and doing exactly what you're doing!

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OfflineNunbuh_Chrubble
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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: jeetered]
    #8673364 - 07/23/08 07:30 PM (16 years, 5 months ago)

you should probably post this in the Advanced Mycology section..

this forum is for n00bs.


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OfflineRogerRabbitM
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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: jeetered]
    #8673366 - 07/23/08 07:31 PM (16 years, 5 months ago)

It's usually with few exceptions, best policy to transfer and isolate rhizomorphic growth with species that exhibit such growth.  You are correct in that monokaryotic mycelium will generally not bruise blue when damaged.
RR


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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: Nunbuh_Chrubble]
    #8673442 - 07/23/08 07:52 PM (16 years, 5 months ago)

Quote:

Nunbuh_Chrubble said:
you should probably post this in the Advanced Mycology section..

this forum is for n00bs.




no it isn't and that's not an advanced myco question. they'd just move it here.

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OfflineNiko
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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: RogerRabbit]
    #8673561 - 07/23/08 08:22 PM (16 years, 5 months ago)

Thanks!

Don't know if it's spectacular enough for Advanced Mycology but I'll continue with the 3 diffrent types I got now (derived from single fluffy mycelium growing very slowly, from contact zone of 2 fluffy mycelia growing average and this rhizomorphic stuff growing quite fast) and see how they develop further as well as prepare one jar mixing everything together.
Let's see what happens...

I'm gonna store some of the rhizomorphic mycelium in the fridge. Is 4°C (40°F) fine or still too much to stop it from growing?
Will it survive the freezer at -20°C (-4°F)?

By the way, it's Astoria Ossip strain, no idea if there's any difference to Hammond...

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OfflineRogerRabbitM
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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: Niko]
    #8673711 - 07/23/08 09:06 PM (16 years, 5 months ago)

Quote:

By the way, it's Astoria Ossip strain, no idea if there's any difference to Hammond...




Hammond, OR is only a few miles from Astoria, OR, so any difference is very little if any.  Both are right next door to Stevens park, where most wild azures are picked from the dune grasses at the beach/woods interface.

Don't freeze live mycelium.  Few species can survive freezing unless on fully colonized wood.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: Niko]
    #8673717 - 07/23/08 09:08 PM (16 years, 5 months ago)

Quote:

Niko said:
Thanks!

Don't know if it's spectacular enough for Advanced Mycology but I'll continue with the 3 diffrent types I got now (derived from single fluffy mycelium growing very slowly, from contact zone of 2 fluffy mycelia growing average and this rhizomorphic stuff growing quite fast) and see how they develop further as well as prepare one jar mixing everything together.
Let's see what happens...

I'm gonna store some of the rhizomorphic mycelium in the fridge. Is 4°C (40°F) fine or still too much to stop it from growing?
Will it survive the freezer at -20°C (-4°F)?

By the way, it's Astoria Ossip strain, no idea if there's any difference to Hammond...




well, im just excited to see you come through with something, I just have one question...

isn't it too late in the year to start a colonized bed?

and if it is, does that mean it wont be this winter you get results, it would be next?

Am i correct?

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OfflineNiko
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Re: P. azurescens mycelium only blueing if dicaryotic? [Re: jeetered]
    #8677269 - 07/24/08 05:39 PM (16 years, 5 months ago)

Well, defines what you mean by too late...
I think it's never too late except when its freezing already, however you're right, the chance that the beds will fruit this year are very small.

No matter, I'm in no hurry.
Besides I don't live in Oregon but in southern middle europe, temperatures usually stay above freezing point most of the time except Jan/Feb.
There should be good growing conditions until mid November and good fruiting conditions till Jan, cool and moist autumn and winter.

The rhziomorphic mycelium has grown about 1/4cm overnight and exhibits feathery growth upwards into the air - very cool!
I'll put some of it in the fridge at 4°C.

I shook all other colonized jars a bit, no blueing visible anywhere else so far.

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