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Offlinejohnnyboy
Bastard Son

Registered: 04/02/07
Posts: 644
Last seen: 11 years, 9 months
an unorthadox G2G question
    #8672440 - 07/23/08 03:40 PM (15 years, 7 months ago)

i inoculated 7 grains jars about nine days ago with a MS inoculation.  yesterday went to check on them and 4 of them were doing really well (id say 50-75% colonized)  so i shook them.  but the other 3 weren't doing so well (they were only about 10% colonized or so) I didn't shake those.

-what I'm wondering is: i have 3 grain jars that just became 100% colonized that I'm going to use for my next batch of jars as a G2G transfer.  can i use the extra left over from that g2g and add some colonized grain to the 3 jars mentioned above to try and get those jars to colonize?


--------------------
I keep the bible in a pool of blood so that none of it's lies will affect me


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InvisibleKrez
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Registered: 01/08/08
Posts: 1,504
Re: an unorthadox G2G question [Re: johnnyboy]
    #8672594 - 07/23/08 04:15 PM (15 years, 7 months ago)

you shouldnt have to add grains to it.  itll be fine just give it some time.


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)


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InvisibleTomandjerry58
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Registered: 01/27/03
Posts: 5,212
Re: an unorthadox G2G question [Re: Krez]
    #8672615 - 07/23/08 04:20 PM (15 years, 7 months ago)

yes you could... its high risk for a contam.... try it~!!!


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InvisibleKrez
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Re: an unorthadox G2G question [Re: Tomandjerry58]
    #8672621 - 07/23/08 04:21 PM (15 years, 7 months ago)

if its a high risk for contam why would he try it...there is growth in the jars just leave it.


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)


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InvisibleTomandjerry58
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Posts: 5,212
Re: an unorthadox G2G question [Re: Krez]
    #8672681 - 07/23/08 04:33 PM (15 years, 7 months ago)

its unorthadox but it can be done.... i always like to work with the fastest jars also. For example if i  innoc 5 jars and 2 of the grow super fast and the other three are going slow. I will use the two that were the fastest for g2g so those will be speedy also.

You don't want to inoculate a bulk substrate with a slow moving strain. Im not saying its impossible but if you can select your speed you choose the fastest.

so personally i wouldn't even use the slow jars.. but thats just prefrence and experience.... maybe for an outdoor plot or somethin.


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InvisibleKrez
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Re: an unorthadox G2G question [Re: Tomandjerry58]
    #8672696 - 07/23/08 04:38 PM (15 years, 7 months ago)

Yea I see what you sayin.  If you do it, do it in a glovebox or try out the oven tek.


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: an unorthadox G2G question [Re: Krez]
    #8672721 - 07/23/08 04:43 PM (15 years, 7 months ago)

Quote:

Krez said:
or try out the oven tek.




hahaha.  no dont try that.  ive had mild sucess with doing this exact same thing in the past with some jars that were extremly slow colonizers.  you can try it but def do it in a glovebox and wipe down all the jars (master and recieving jars) good with alcohol.

but like was mentioned above, give it a little more time first and see what happens.


Edited by blood4blood (07/23/08 04:44 PM)


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InvisibleKrez
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Re: an unorthadox G2G question [Re: blood4blood]
    #8672854 - 07/23/08 05:29 PM (15 years, 7 months ago)

hey dont knock the oven tek...:)  before i had my glovebox thats what i did and had damn good success.  i guess that depends on how clean you keep your place.  gloveboxs are easy to make and and cheap.  plenty of teks here for em...yea i guess your right in knockin the oven tek...Nevermind bout that oven tek, my bad!


--------------------
Kids, you tried your best and you failed miserably. The lesson is, never try.  (Homer)

Look, the thing about my family is there's five of us. Marge, Bart, Girl Bart, the one who doesn't talk, and the fat guy. How I loathe him. (Drunk Homer)

All the ways you wish you could be, that's me. I look like you wanna look, I fuck like you wanna fuck, I am smart, capable, and most importantly, I am free in all the ways that you are not. (Tyler Durden)


Edited by Krez (07/23/08 05:37 PM)


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