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Kada
Asha'man


Registered: 02/15/05
Posts: 12,395
Loc: Buckeye
Last seen: 9 months, 25 days
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I cut corners at every step, yet i never get contaims
#8662735 - 07/21/08 11:23 AM (16 years, 5 months ago) |
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I slowly stopped doing alot of the things a person would do to prevent contaimination. The only thing i do to prevent contaims is to steam sterilize my jars for 90 minutes. I even cut out boiling water for my syringe, i just use filtered water from my fridge. I don't use a glove box, i just wash my hands. I do boil my needle before i reuse it for 10-15 minutes, but thats about it.
When i make a syringe, i just scrape my print into a clean cup, then fill it with water from the fridge and suck it up into the syringe. Im going to start doing the mycelium syringe tek, because it's aot faster colinization.
Am i just getting lucky here, or is making your jars right, then sterilizing your syringe between uses really all someone needs to do? I have followed every safty method RR's vid shows, and i have disregaurded almost every sterile tek in his vid. I never get contaims.
Luck or something else? My house is clean, i take 2 showers a day, and i handle my materials carefully. I just never bother with glove boxes, gloves, masks, alcohol, lysol, air purifiers, or flow hoods. I do pf tek brf jars.
-------------------- ~The Cultivators Motherload~
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them.
I am free because I know that I alone am morally responsible for everything I do." -Robert A. Heinlein
"There is no need for temples, no need for complicated philosophies.
My brain and my heart are my temples; my philosophy is kindness."-Dalai Lama
Live long and prosper.
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BrandNewbie
Captain



Registered: 05/21/08
Posts: 2,932
Loc: U.S.A.
Last seen: 5 years, 7 months
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Re: I cut corners at every step, yet i never get contaims [Re: Kada]
#8662792 - 07/21/08 11:35 AM (16 years, 5 months ago) |
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Can you do my shit for me?
-------------------- Question: Why do women wear make-up and perfume?
Answer: Because they're ugly and they stink.
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dirtworshipper
Sitting in the heart cave



Registered: 05/12/07
Posts: 2,060
Loc: at The Guru's lotus feet
Last seen: 4 years, 2 months
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Re: I cut corners at every step, yet i never get contaims [Re: Kada]
#8662801 - 07/21/08 11:37 AM (16 years, 5 months ago) |
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I'm going to have to say that you're pretty lucky. I doubt you'd have as much luck with grains, unfortunately.
But congratulations for all of the success Who cares if it's luck or not in the successful end?
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“You've got as many lives as you like, and more, even ones you don't want.” - George Harrison
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moho456
The Past Inside The Present



Registered: 06/10/06
Posts: 223
Loc: Translinguistic Matter
Last seen: 10 years, 2 months
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Re: I cut corners at every step, yet i never get contaims [Re: dirtworshipper]
#8662816 - 07/21/08 11:41 AM (16 years, 5 months ago) |
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I'm a total slob, I work with grains, and I cut corners with great success too.
Maybe its a combination of environment, luck, and established paranoia.
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seven
.


Registered: 06/09/08
Posts: 1,478
Loc: north carolina
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Re: I cut corners at every step, yet i never get contaims [Re: moho456]
#8663254 - 07/21/08 01:18 PM (16 years, 5 months ago) |
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id say environment factors play a big roll in contams. i know older houses have alot of contams. moldy ass old houses . haha! air movement is a factor. most people go overboard just to be on the safe side. which is a good idea. i plan on useing grains on my first grow so i will be very carefull. also i doubt you will ever advance in mycology cutting corners like that. other aspects of this hobby are less forgiving. ie: agar work. id say you are lucky. but you can take my words with a grain of salt so to speak.
-------------------- grind
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: I cut corners at every step, yet i never get contaims [Re: seven]
#8663289 - 07/21/08 01:27 PM (16 years, 5 months ago) |
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Quote:
Luck or something else? My house is clean, i take 2 showers a day, and i handle my materials carefully. I just never bother with glove boxes, gloves, masks, alcohol, lysol, air purifiers, or flow hoods. I do pf tek brf jars.
I clean my area around me before I work.. like bleach wipe the counter, a lil lysol in the air.. stop any fans, etc.. I NEVER wear gloves except when I am handling and mixing my coir.. just a precaution.. I use alcohol and PC any metal instruments I will use.. I just stick them in with my jars.. killing 2 birds, ya know?
Im all about being clean, but I never use my glove box unless my cats are shedding real bad, or im working with agar..
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Izord
Stranger



Registered: 02/05/08
Posts: 44
Last seen: 15 years, 3 months
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Me too. I cut corners at every step, yet i never get BAD contams [Re: DJYoshaBYD]
#8663489 - 07/21/08 02:34 PM (16 years, 5 months ago) |
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I've been doing it the easy way for years. Don't own Lysol or Chlorox. I use filter disks on my grain jars and shoot my spores thru there with a needle which I never flame. I do use a cool a technique which I think I thought of. It is that I pressure cook my grain twice. Once to activate the endospores and once to kill them on the next day. I never soak my grain. Just add a fixed quantity of grain and water to my jars. Too fucked up right now to tell you exactly how much, but I use quart jars. I wrote it down somewhere, anyway. I add water and grain, pc, wait till the next day and pc again. So I never soak or even rinse my grain.
I get about 9 out of 10 good jars and take the bad one outside and dump the mold into my forest then rinse the jar with the hose then take it inside and put it in the dishwasher.
I'll say it again, I don't own lysol or chlorox and don't use any alcohol to sterilize anything when I grow.
I get great harvests, enough for me and all my relatives. I keep in touch with the mother for regular communications and advice. : ) I'll try some different strains now and then for some hardy ones and I've been lucky enough to avoid bad contams.
I feel sorry for people with contamination problems. After learning how to grow from a guy who was crazy about spraying the air with chlorox and the table with lysol and wearing a mask while he worked with strains and shit like that, I had to find a better way for myself.
Iz.
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themange
Senior Citizen



Registered: 06/11/06
Posts: 2,809
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Re: I cut corners at every step, yet i never get contaims [Re: DJYoshaBYD]
#8663504 - 07/21/08 02:40 PM (16 years, 5 months ago) |
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Quote:
DJYoshaBYD said: I clean my area around me before I work.. like bleach wipe the counter, a lil lysol in the air.. stop any fans, etc.. I NEVER wear gloves except when I am handling and mixing my coir.. just a precaution.. I use alcohol and PC any metal instruments I will use.. I just stick them in with my jars.. killing 2 birds, ya know?
same here . if you critically think about sterility you can pinpoint what needs to be clean and what doesnt.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Me too. I cut corners at every step, yet i never get BAD contams [Re: Izord]
#8663534 - 07/21/08 02:47 PM (16 years, 5 months ago) |
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pressure cooking doesnt "activate the endospores"... lol.. you could probably get a 10 out of 10 if you you used it right... lol
I mean, there IS a reason that you take sterile precautions.. so you have 100% success...
his way was not bad at all.. i mean, in a way, your way is slightly careless.. you shouldnt need to PC twice, but hey.. if it works, I guess it works.. I would for sure chalk up your success with almost no contams as luck...
it also really depends on your living environment.. sure you dont lysol and stuff right away, but I bet your house isnt a pig sty either.. really, as long as you turn off the fans and dont have too much moving air around your all good..
you shoot the spores through the filter, but I wonder how well this technique you use would work if you got out of using spores for every grow and started using a live culture.. you would have to crack open the lid, and then I bet 10 bucks that you would get contams on every jar you opened if you didnt sterilize the air...
the reason your jars, after sterilizing, dont get contams, is because you are not exposing them to the air.. just a dirty needle.. at least alcy the needle, and that may bring you up to 100% success.. but if you are working on any substrate or tek that you need to open the jar to get stuff in, then it will most likely contam..
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psylopsycho
Motivational Speaker



Registered: 06/22/08
Posts: 503
Loc: The Tundra
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Re: Me too. I cut corners at every step, yet i never get BAD contams [Re: DJYoshaBYD]
#8663726 - 07/21/08 03:47 PM (16 years, 5 months ago) |
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I would say congratulations on all the luck that you have had. Although I think that this post may leave alot of noobs a bit unhappy if they start throwing sterility to the wayside.
Being a bit OCD'ish when it comes to mycology seems to be a staple.
I think I will stick with sterility!
Congrats though!
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Someone actually said: "some 1 told me to try long brown rice and water and vermiculite and pressure cook for 1 hour i did but nothing happen that is y was wonting to no if i need spores ""
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Phraggle
Psilocybe Therapy Advocate


Registered: 04/19/06
Posts: 161
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Re: I cut corners at every step, yet i never get contaims [Re: Kada]
#8664096 - 07/21/08 05:36 PM (16 years, 5 months ago) |
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Wow. You must be a complete noob or something since according to some people it is absolutely impossible to cut corners and have success.
-------------------- You'll see me.
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 11 months
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Re: I cut corners at every step, yet i never get contaims [Re: Phraggle]
#8664128 - 07/21/08 05:47 PM (16 years, 5 months ago) |
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sterile technique = 
Edited by shevanel (07/21/08 05:50 PM)
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MarioTrip
since84



Registered: 10/21/07
Posts: 2,207
Loc: 69 w/ you
Last seen: 16 years, 3 months
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Re: I cut corners at every step, yet i never get contaims [Re: shevanel]
#8664232 - 07/21/08 06:13 PM (16 years, 5 months ago) |
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you can get away with sloppiness for awhile but not forever.
-------------------- I'm Livin' It, distribute it
The streets are inhibited
By cut throat niggas just like me
that out there getting dividends
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psylopsycho
Motivational Speaker



Registered: 06/22/08
Posts: 503
Loc: The Tundra
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Re: I cut corners at every step, yet i never get contaims [Re: MarioTrip]
#8664304 - 07/21/08 06:38 PM (16 years, 5 months ago) |
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Quote:
MarioTrip said: you can get away with sloppiness for awhile but not forever.
Amen Brother
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Someone actually said: "some 1 told me to try long brown rice and water and vermiculite and pressure cook for 1 hour i did but nothing happen that is y was wonting to no if i need spores ""
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Jenkins22
Mutilating_cows_4_better_poo

Registered: 06/26/08
Posts: 223
Loc: Fuk_U_Earth
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Re: I cut corners at every step, yet i never get contaims [Re: psylopsycho]
#8664358 - 07/21/08 06:50 PM (16 years, 5 months ago) |
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if u get a sealed needle from the pharmacy for like 10 cents then there is no need to sterilize if u use it right out the pack. They're already sterile.
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Mad River
Reverend, Churchof Todd



Registered: 04/29/08
Posts: 1,114
Loc: The Great Lakes Region, U...
Last seen: 3 years, 6 months
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Re: I cut corners at every step, yet i never get contaims [Re: Jenkins22]
#8664399 - 07/21/08 06:59 PM (16 years, 5 months ago) |
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And if you buy crunchy peanut butter, you don't have to mix in your own nuts-- they're already in there! 
I learned this the hard way.
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ray40cal
omnitrippint



Registered: 05/05/08
Posts: 1,308
Loc: midwest side
Last seen: 16 years, 1 month
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Re: I cut corners at every step, yet i never get contaims [Re: Mad River]
#8664485 - 07/21/08 07:14 PM (16 years, 5 months ago) |
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I poop in my terrarium. But really, i don't even use tyvek or anything like that, my PC only goes up to 10psi, only time lysol is in the air is for grain to grain work, never set the pH on my casing layers... Only contam issue I have had to deal with (once) was casing layer after the 2nd flush. I even put bugs from my backyard into the FC to eat fruit flies, gnats or whatever those fuckers are.
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Izord
Stranger



Registered: 02/05/08
Posts: 44
Last seen: 15 years, 3 months
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Re: Me too. I cut corners at every step, yet i never get BAD contams [Re: DJYoshaBYD]
#8677292 - 07/24/08 05:46 PM (16 years, 5 months ago) |
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Hey DJYoshaBYD,
You said
"pressure cooking doesn't "activate the endospores"... lol.. you could probably get a 10 out of 10 if you you used it right... lol"
It's not the pressure cooking, it's the heat and moisture that break their shell and start them growing. Endospores are created to resist times of poor environment so the bacteria can survive. Warmth and moisture and maybe nutrients available signal them to start growing. That's why people soak their grain overnight before pc'ing.
I just found it's easier not to soak at all. Just add ingredients to my jars, put in pressure cooker and pc for an hour then wait till the next day and pc again. I don't even take them out of the pc. Consider that the easy way to soak. And the first pc kills all the stuff outside the endospores, then the signal for the endospore to hatch, just like soaking, and then pc again the next day before new endospores form. It's just like soaking and rinsing, except easier, because it skips those two time consuming steps. I guess it takes me about two minutes to turn off the stove and turn it on again the next day, but I use a timer to clock the actual pc time.
So LOL right back at you, yes my pc technique does "activate" and KILL the endospores.
And their isn't any need for me to flame my needles. I use sterile ones, and cap them, and replace them after a few times. The filter disks they go through are sterile because I keep the pressure cooker closed till the jars are cool and I'm ready to inoculate.
And you are right that I'm successful because I keep the jars closed, I never take off the filter disk till I'm ready to spawn, and I've never had a spawn go bad at that point. I can just look at my jars and see the nice fluffy white ones and by that time they are ready to run the spawn.
The technique requires a Little patience to let that pc sit somewhere overnight with jars inside. But I don't want to work unnecessarily and chunking one jar out of 10 is a lot easier on me that going crazy with Lysol, chlorox, and alcohol. The alcohol belongs in my belly not on a rag to wipe the table. : ) And fuck rinsing grain and soaking. What a hastle!! and Unnecessary. Unless you went to get a prize in Guinness book of world records for least contams. (Or you think toil is a virtue.)
Moderation in everything buddy.
Peace.
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Izord
Stranger



Registered: 02/05/08
Posts: 44
Last seen: 15 years, 3 months
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Re: I cut corners at every step, yet i never get contaims [Re: MarioTrip]
#8677298 - 07/24/08 05:48 PM (16 years, 5 months ago) |
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Quote:
MarioTrip said: you can get away with sloppiness for awhile but not forever.
How about since 1984? Is that close to forever? Or more than awhile?
Iz : )
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 11 months
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Re: Me too. I cut corners at every step, yet i never get BAD contams [Re: Izord]
#8677481 - 07/24/08 07:09 PM (16 years, 5 months ago) |
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Quote:
Izord said:
I just found it's easier not to soak at all. Just add ingredients to my jars, put in pressure cooker and pc for an hour then wait till the next day and pc again. I don't even take them out of the pc.
Consider that the easy way to soak. I guess it takes me about two minutes to turn off the stove and turn it on again the next day, but I use a timer to clock the actual pc time. .
That sounds lazy & cool but assuming your PC doesn't handle over 20 quarts, who knows but you , I think it's easier to soak 2-3 5 gallon buckets of seed in mesh bags overnight then start running the PC in batches thereafter.
I suppose your method works if your making grain for spawn masters or just a small grow, thats fine.. but if you're trying to nock out more than 10ish jars or so soaking in batches doesn;t seem more wastefull than spending almost 2 days to completely do 1 pc cycle.
I don't understand how anyone would want to Pc jars then wait until the next day to pressure cook the SAME jars again... afterall, isn't that nearly same amount spent soaking grain for 24 hours?
I'm very confused.
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