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Offlinetrower
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Registered: 05/01/08
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2 of 4 jars stalled?
    #8657641 - 07/20/08 03:36 AM (15 years, 8 months ago)

Made 4 jars of cracked corn (experimenting from reading older teks) All 4 quarts made from same batch of soaked, simmered, rinsed well, drained, pcd, well cooled corn, then knocked up with homemade syringes of GT (10cc each) form a large print, each syringe had great color, micropore taped innoc. sites, stored in same place at same temp (85), after 30%25 colonization gave each a good shake, and returned to same incubation area. after 4 more days 2 of the jars are about 95%25 and the other 2, nuttin just need more time? or did something go a rye?(sorry just couldnt help myself) PEACE

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OfflinePowerOfTheCoir
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Registered: 07/02/08
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Re: 2 of 4 jars stalled? [Re: trower]
    #8657783 - 07/20/08 05:41 AM (15 years, 8 months ago)

No visible contamination but a failure to recover after shaking usually indicates bacterial contamination. If the jars do eventually start going again, keep them separate from the others when you use them for spawn or whatever. They may fight the contaminants back, but there will be a good chance the bad guys will return at later date.

What kind of lid did you use?

Also, "great color," if that means dark, can often be undesirable in a syringe. Do a search for "dark syringes" and you'll see why. And did you mean that there were 10cc's in each syringe, or did you put 10cc's in each jar? If you put 10cc's in each jar, that's a lot of extra water.

One more thing - it may not be causing your problem, but 85 is hot for colonization. That temp is often listed as ideal, but that was for thin agar dishes. With a jar, the myc builds considerable internal heat and the center is significantly hotter than the outside of the jar. Many people are now going to room temperature colonization.


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