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djblackout
supreme scratch masta


Registered: 05/03/08
Posts: 1,870
Last seen: 14 years, 4 months
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nichrome loop
#8647673 - 07/17/08 03:48 PM (16 years, 6 months ago) |
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hey i did a search but havent found much material... can you really just scrape some of the colonization out and xfer it into another jar?
anyone know good writeups on this?
-------------------- I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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yes yes yes.. its called a live mycelium transfer, live culture transfer/inoculation, etc etc etc
just, in the cleanest way possible, take a chunk of fungus, and put in a jar of sterile whatever (brf, wbs, etc)..
I have used the same strain for a while now, and all I did was use a colonized jar to noc up other jars... its very simple..
again.. just, very clean, take a chunk of mycelium, and put it in a jar of your favorite substrate, and watch it grow..
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djblackout
supreme scratch masta


Registered: 05/03/08
Posts: 1,870
Last seen: 14 years, 4 months
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so lets say i have a jar thats 100% done, just take a chunk and transfer it to a BRF jar? and then put some verm on top? it just seems like it would contam. Id be doing it in a glovebox. i just thought u could never open the jars after you sterlize them
-------------------- I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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you could rig up a lid with a tyvek filter instead of a verm layer filter to do this to cakes. there is a small contam risk but I've done a lot of G2Gs that worked out fine this shouldn't be much different. I mean there's never a 0% contam risk. I'd say do that and smooth a small layer of spawn over the top and pop the lid back on.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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I never used verm on top of my brf cakes..
I would use a couple of coffee filters (fuck the ney-sayers) and open the jar in a glove box..
I open my jars to do a live myc transfer.. the thing about live myc transfer, is the fact it starts growing within 24 hours, as opposed to noc-ing up a jar with spores, that can take more than a week to show growth..
if you are sterile, then you shouldnt have to worry about opening the jars... you pretty much HAVE to, to get the fungus in..
just only open it a lil bit.. just enough to fit your tweezers with your fungus in.. dont breathe in it, etc..
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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no contams like this.. with the jars like this, I can open the jar just slightly to get the transfer in there.. none of these jars here got contaminated... just keep them in a clean spot, and DONT BREATE IN THE HOLE!! lol. everyone says they wont keep contams out, but if you are careful enough not to take them outside, pass them around the room to your friends, sing in to them ,etc., then they work... all the time...
edit: just to clarify; the filters are in between the 2 pieces of lid.. the first cover (with a hole in it) goes on, then the filter, then the ring around it to lock it in place.. just thought I would be a little more concise..
Edited by DJYoshaBYD (07/17/08 04:17 PM)
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djblackout
supreme scratch masta


Registered: 05/03/08
Posts: 1,870
Last seen: 14 years, 4 months
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so what your saying is all i have to do is..
get coffee filters, make a brf jar with no dry verm, drop a sample in and prolly cover it up with the brf. and thats it?
hey could i use a cake that has already been flushed once and dunk and roll to do these jars?
-------------------- I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You
Edited by djblackout (07/18/08 02:23 AM)
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ScavengerType



Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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I've heard that already flushed material is more prone to contams and weaker for using as spawn, but otherwise yes.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
Edited by ScavengerType (07/18/08 02:50 AM)
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djblackout
supreme scratch masta


Registered: 05/03/08
Posts: 1,870
Last seen: 14 years, 4 months
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i must try.
-------------------- I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You
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aspore
myconaut



Registered: 07/10/07
Posts: 663
Last seen: 12 years, 4 months
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i do not blieve you can use Mycelium that has flushed. After it starts to pin, its desire to grow more MYC is gone I believe, it then focuses on producing fruits.
So if you want this to work, you MUST use a jar that is still colonizing.
Also, contams are not a big issue for everyone. But for those of use who have contam issues, it's VERY annoying to do a project, and have green creep in 2 days later. Such a setback.
I would do the WATER MYCELIUM TEK that is on the front page right now. This is more sterile, and gives same results, if not better.
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c l i c k m e
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