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OfflineWhiteBunny
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Agar questions
    #8647458 - 07/17/08 03:07 PM (16 years, 6 months ago)

So I think I want to use this recipe
Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)


My question is if I don't have a scale how much is 150grams of potatoes.  I can weight it on the scale at the store but it's not going to be in grams.  Also I have agar agar but need to know a American measurement to mix.  Any help would be great.  Thanks

WB


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OfflineWhiteBunny
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Re: Agar questions [Re: WhiteBunny]
    #8648292 - 07/17/08 06:40 PM (16 years, 6 months ago)

I got the Potatoes measurement taken car of but I don't know much 9 grams of agar is or 10 grams of corn syrup are because they have different densities... any help?  Looking for something like... 1/2 a tea spoon or something.

Also I picked up a nice thick favor injector from the grocery and I am think of using it to extract mycelium from the LC's.  Has anyone used it?  IF I pressure cook it to sterilize it will it melt?


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Edited by WhiteBunny (07/17/08 06:49 PM)

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InvisibleFooMan
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Re: Agar questions [Re: WhiteBunny]
    #8648307 - 07/17/08 06:45 PM (16 years, 6 months ago)

I'm not sure about how to measure the agar without a scale, but as far as the corn syrup goes, use a 10cc syringe. 10ml's is the same as 10cc's. Fill the syringe with corn syrup and you have the correct amount.


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OfflineWhiteBunny
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Re: Agar questions [Re: FooMan]
    #8648318 - 07/17/08 06:50 PM (16 years, 6 months ago)

Don't have any empty syringes :frown:

WB


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InvisibleFooMan
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Re: Agar questions [Re: WhiteBunny]
    #8648328 - 07/17/08 06:58 PM (16 years, 6 months ago)

PM me your address and I'll mail you one. I would order a scale off of ebay while you wait for it :smirk:


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InvisibleLedd
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Re: Agar questions [Re: FooMan]
    #8648406 - 07/17/08 07:37 PM (16 years, 6 months ago)

Come on man, you can't find conversion tables?

:google:


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OfflineRogerRabbitM
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Re: Agar questions [Re: Ledd]
    #8648422 - 07/17/08 07:43 PM (16 years, 6 months ago)

If you don't have scales or empty syringes, you probably also don't have petri dishes, a flowhood or good glovebox, surgical masks and gloves, scalpels, etc., that are all important for agar isolations.  Get your supplies together first.

Everyone with a nickel and a ruler has a scale.  Balance a ruler over a fulcrum at the 6" mark, and place a nickel at the 11" mark and your powder at the 1" mark.  When it balances, you have 5 grams of product.
RR


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OfflineNo_Life_G33k
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Re: Agar questions [Re: RogerRabbit]
    #8648428 - 07/17/08 07:45 PM (16 years, 6 months ago)

where do I buy a 'fulcrum'?????


:wink:


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Re: Agar questions [Re: No_Life_G33k]
    #8648435 - 07/17/08 07:47 PM (16 years, 6 months ago)

A fulcrum is just a pivot point, like your elbow or knee, or the thing in the center of a see-saw. Use a coke can or prism...


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OfflineWhiteBunny
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Re: Agar questions [Re: Ledd]
    #8650159 - 07/18/08 08:30 AM (16 years, 6 months ago)

Eye Balled it last night, good chance it wo't work but I can always get more supplies.  Ordered a scale last night too. 

Any idea on the favor injector to suck up my LC?  IF I wrap the injector in tin foil and pc it will it melt?

WB


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Offlinerobanero
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Re: Agar questions [Re: WhiteBunny]
    #8650197 - 07/18/08 08:51 AM (16 years, 6 months ago)

Mine did.


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OfflineP.Menace
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Re: Agar questions [Re: robanero]
    #8650240 - 07/18/08 09:06 AM (16 years, 6 months ago)

mine broke with alcoho inside... that was WAY back now tho so they might make em different now


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OfflineNo_Life_G33k
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Re: Agar questions [Re: P.Menace]
    #8650385 - 07/18/08 10:07 AM (16 years, 6 months ago)

Does it have the little recycling symbol on it???

look for a 5 inside a triangle. 5 is your friend.


--------------------
Hot Water Pasteuriztion - A How To Pictorial
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4527808/an/0/page/0

Make your own shroom chocolates
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4500682/an/0/page/5

guide with pix.


Make Your Own Magnetic Stirrer! How-To Tek w/ pix
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5865949/an/0/page/3

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Offlinechobumms
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Re: Agar questions [Re: No_Life_G33k]
    #8650425 - 07/18/08 10:23 AM (16 years, 6 months ago)

10 mls is two teaspoons
9 grams of agar is one bar of agar you get at the chinese grocery stores that come in a two pack.


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