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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Vermiculite

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OfflineThe_Greater_God
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Registered: 08/24/02
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Can syringes be used more than once ?
    #864320 - 09/04/02 09:27 PM (14 years, 3 months ago)

If sterlized and using the same species, can your inocculation syringes be used more than once ?


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OfflinegeokillsA
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Re: Can syringes be used more than once ? [Re: The_Greater_God]
    #864325 - 09/04/02 09:30 PM (14 years, 3 months ago)

of course, just fill 'em up with boilin' water, let it sit for a minute, purge out the water, repeat two or three times - the last time leaving the water in the syringe and let it cool.  now ya got a sterile syringe with sterile water in it.  you can even mix your sterile water with a different strain and suck it back up :cool:
 


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OfflineThe_Greater_God
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Registered: 08/24/02
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Re: Can syringes be used more than once ? [Re: geokills]
    #864381 - 09/04/02 09:55 PM (14 years, 3 months ago)

Ok, lets suppose I had a spore print of a B+ strain and the cap measured 4x4 inches, how much of the spores do I put in per 10cc's ? Is there a formula/equation for this ?


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OfflinegeokillsA
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Re: Can syringes be used more than once ? [Re: The_Greater_God]
    #864388 - 09/04/02 09:59 PM (14 years, 3 months ago)

as much as you want.. no forumula, it's just a personal choice


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InvisibleRipple
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Re: Can syringes be used more than once ? [Re: geokills]
    #864674 - 09/05/02 04:52 AM (14 years, 3 months ago)

That's a lot of spores for 10 cc do you feel that it speeds up colonization?


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OfflinegeokillsA
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Re: Can syringes be used more than once ? [Re: Ripple]
    #878366 - 09/11/02 04:44 PM (14 years, 2 months ago)

more spores spread around your substrate = quicker colonization

but of course, fewer spores will work (possibly just as well), so like i said before ~ it's all personal preference


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OfflineShadler77
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Registered: 09/10/02
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Re: Can syringes be used more than once ? [Re: geokills]
    #878839 - 09/11/02 07:56 PM (14 years, 2 months ago)

I have a question as far as syringe preparation and sterilization. What's the proper process and precautions to take? I have an empty syringe that's a bit dirtied, by vermiculite....wondering how I should clean it. Also, how long and in what conditions does a spore syringe last? Thanks for any help....


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Offlineblackout
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Re: Can syringes be used more than once ? [Re: Shadler77]
    #879127 - 09/11/02 11:43 PM (14 years, 2 months ago)

if you can see spores in a syringe then you have enough.

just clean the syringe in hot water. i microwave mine in a tall pint glass with a plate on top to stop them floating about. i suck up and purge boiling water several times. then i put them back in the microwave for 20mins


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Invisiblematts
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[Re: blackout]
    #879136 - 09/11/02 11:48 PM (14 years, 2 months ago)



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InvisibleCptnMaxMushroom
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Registered: 08/26/02
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Re: Can syringes be used more than once ? [Re: Shadler77]
    #879232 - 09/12/02 01:10 AM (14 years, 2 months ago)

You should clean your used syringes properly. Simply sucking up some boiling water is not good enough. See the rubber end on the plunger? There is a grove in that rubber which is almost surely filled with old spores. There is alos likely a ring of old spores stuck in the bottom of the barrel.

Take the syringe apart and soak in warm soapy water for a while. Now wipe that rubber ring with some tissue. Take a small square of tissue about the szie of a quarter and push it down into the barrel with the plunger. Twist the lunger around a couple times so the inside bottom of the barrel gets cleaned by the tissue.
Now pressure cook the parts for just a short time and with very clean or gloved hands, put back together. I suggest you do this cleaning process immediately before using the syringes.


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Invisiblemickey_rourke
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Re: Can syringes be used more than once ? [Re: The_Greater_God]
    #879415 - 09/12/02 02:53 AM (14 years, 2 months ago)

I PC all of my syringes before reuse. I don't suffer from contamination problems in syringes I make. The only problems I've ever had were with vendor made syringes.


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Offlineshroomsformike
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Registered: 08/13/02
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Re: Can syringes be used more than once ? [Re: The_Greater_God]
    #880636 - 09/12/02 03:06 PM (14 years, 2 months ago)

this is all I did to sterilize my syringe?s:


$19.19 for a box of 100/ 3 m/l but i use one on each jar now


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Can syringes be used more than once ? [Re: The_Greater_God]
    #880854 - 09/12/02 05:18 PM (14 years, 2 months ago)

Just wrap them in aluminumfoil and pressure cook for 25 minutes. Works for most types and brands. Just sucking up hot water will not sterilise a syringe.


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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