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Offlinepeterr1211
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Registered: 05/23/08
Posts: 89
Last seen: 12 years, 6 months
what is this yellow and purple stuff?
    #8635916 - 07/14/08 11:46 PM (15 years, 8 months ago)

can anyone help me on what went wrong here?


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: what is this yellow and purple stuff? [Re: peterr1211]
    #8635977 - 07/15/08 12:14 AM (15 years, 8 months ago)

Moulds by the looks of them. The second one appears to be Neurospora.

Quote:

Pink Mold; Red Bread Mold – Neurospora

    Commonly to occasionally seen on agar and grain. Neurospora is fast growing, sometimes taking only 24 four hours to totally colonize a media filled petri dish. It is ubiquitous in nature, occurring on dung, in soils and on decaying plant matter. Since this fungus grows through cotton stoppers or filter discs, a single contaminated jar, though sealed, can spread spores to adjacent spawn jars within the laboratory. This condition is more likely if the filter discs or cotton plugs are the least bit damp; or if the external humidity is high. Furthermore, Neurospora spores germinate more readily at elevated temperatures. The pink mold seen in mushroom culture is most frequently Neurospora sitophila, a pernicious contaminant that is difficult to eliminate. All infected cultures should be removed as soon as possible from the laboratory and destroyed. A thorough cleaning of the laboratory is absolutely necessary. If contamination persists, remove all spawn and start anew.




Not sure what the first one is though.

You started from wild prints right?

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Offlinepeterr1211
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Re: what is this yellow and purple stuff? [Re: wisp]
    #8635997 - 07/15/08 12:22 AM (15 years, 8 months ago)

cool, good to know.  I better get rid of the purple one right away so it doesn't spread to my other jars.  And yes I did get these spore from the wild, would that mean its tough to say what the yellow one is for definite.  I guess there are numerous contaminants in the wild that were already on the spores?


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: what is this yellow and purple stuff? [Re: peterr1211]
    #8636012 - 07/15/08 12:27 AM (15 years, 8 months ago)

Yeah you got it right in your last question. Wild prints are likely to be contaminated and they generally need cleaning up on agar.

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OfflineRogerRabbitM
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Re: what is this yellow and purple stuff? [Re: wisp]
    #8636778 - 07/15/08 07:54 AM (15 years, 8 months ago)

Mold on the left, bacteria on the right.  Toss both.
RR


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: what is this yellow and purple stuff? [Re: RogerRabbit]
    #8636792 - 07/15/08 07:59 AM (15 years, 8 months ago)

Out of interest, how do you conclude the one on the right is bacteria?

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OfflineThe shroomy 1
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Re: what is this yellow and purple stuff? [Re: wisp]
    #8638904 - 07/15/08 05:10 PM (15 years, 8 months ago)

I'm guessing by the "greasy", "wet" accumulations surrounding the grains.

The Shroomy 1


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: what is this yellow and purple stuff? [Re: The shroomy 1]
    #8639210 - 07/15/08 06:23 PM (15 years, 8 months ago)

I suppose it doesn't have the powdery appearance that moulds have, but I've not seen purple bacteria before. Not saying they don't exist, just not come across them yet.

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OfflineRogerRabbitM
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Re: what is this yellow and purple stuff? [Re: wisp]
    #8640413 - 07/15/08 11:01 PM (15 years, 8 months ago)

Quote:

I'm guessing by the "greasy", "wet" accumulations surrounding the grains.




Exactly. 

The purple near the top appears to be metabolites.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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