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 3Mshroom
old hand
Registered: 04/03/00
Posts: 497
Last seen: 17 years, 1 month
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 Re: How Many Generations Can You Grow Of Mycelium 
#86377 - 06/20/00 02:36 AM (18 years, 4 months ago) |
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It varies from strain to strain. All you can do is try it and when you see a deterioration in the strain then it is time to go back to spores or clone another shroom------------------
Be sure to check out www.3mshrooms.homestead.com for your spore needs
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Workman
Psilocybe Microscopist
 
Registered: 03/01/01
Posts: 3,563
Loc: Washington, USA
Last seen: 6 hours, 52 minutes
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Re: How Many Generations Can You Grow Of Mycelium
#86379 - 06/21/00 01:22 PM (18 years, 4 months ago) |
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A good rule of thumb is 10 transfers or less without significant degeneration. Alteration of media between transfers helps maintain strain integrity. Some species, such as morels, tend to degenerate faster.------------------
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Anonymous
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Re: How Many Generations Can You Grow Of Mycelium
#86380 - 06/21/00 06:40 PM (18 years, 4 months ago) |
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So in theory, a cultivator could use agar to grain....then back to agar (from the grain) indefinately???? Your are in fact changing the substraight!Just a thought
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/02/00
Posts: 870
Last seen: 3 years, 9 months
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Re: How Many Generations Can You Grow Of Mycelium
#86381 - 06/22/00 06:08 PM (18 years, 4 months ago) |
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I wouldn't say indefinately, it will help, but it won't keep it going 1000000's of times, I have no idea how less signifigant the degredation is, but I assume its not large enough to lead to indefinate continuation.
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Anonymous
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Re: How Many Generations Can You Grow Of Mycelium
#86382 - 06/25/00 04:56 AM (18 years, 4 months ago) |
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I would also agree with Workman,
I never use the first generation for
inoculation I sector the ryzomorphic (ropey)
sectors and transfer to a new agar medium.
I have gotten up to 20 generations from B+
and only 10 from mazatapec. So I think it depends on the strain.
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