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Re: How Many Generations Can You Grow Of Mycelium
    #86377 -

It varies from strain to strain. All you can do is try it and when you see a deterioration in the strain then it is time to go back to spores or clone another shroom

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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86379 -

A good rule of thumb is 10 transfers or less without significant degeneration. Alteration of media between transfers helps maintain strain integrity. Some species, such as morels, tend to degenerate faster.

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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86380 -

So in theory, a cultivator could use agar to grain....then back to agar (from the grain) indefinately???? Your are in fact changing the substraight!

Just a thought


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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86381 -

I wouldn't say indefinately, it will help, but it won't keep it going 1000000's of times, I have no idea how less signifigant the degredation is, but I assume its not large enough to lead to indefinate continuation.


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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86382 -

I would also agree with Workman,
I never use the first generation for
inoculation I sector the ryzomorphic (ropey)
sectors and transfer to a new agar medium.
I have gotten up to 20 generations from B+
and only 10 from mazatapec. So I think it depends on the strain.

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