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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Offline3Mshroom
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Re: How Many Generations Can You Grow Of Mycelium
    #86377 - 06/20/00 12:36 AM (22 years, 11 months ago)

It varies from strain to strain. All you can do is try it and when you see a deterioration in the strain then it is time to go back to spores or clone another shroom

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OfflineWorkmanV
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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86379 - 06/21/00 11:22 AM (22 years, 11 months ago)

A good rule of thumb is 10 transfers or less without significant degeneration. Alteration of media between transfers helps maintain strain integrity. Some species, such as morels, tend to degenerate faster.

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Anonymous

Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86380 - 06/21/00 04:40 PM (22 years, 11 months ago)

So in theory, a cultivator could use agar to grain....then back to agar (from the grain) indefinately???? Your are in fact changing the substraight!

Just a thought



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OfflineWildLASER
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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86381 - 06/22/00 04:08 PM (22 years, 11 months ago)

I wouldn't say indefinately, it will help, but it won't keep it going 1000000's of times, I have no idea how less signifigant the degredation is, but I assume its not large enough to lead to indefinate continuation.


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Anonymous

Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
    #86382 - 06/25/00 02:56 AM (22 years, 11 months ago)

I would also agree with Workman,
I never use the first generation for
inoculation I sector the ryzomorphic (ropey)
sectors and transfer to a new agar medium.
I have gotten up to 20 generations from B+
and only 10 from mazatapec. So I think it depends on the strain.


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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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