Shroomery - Magic Mushrooms Demystified
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3Mshroom
old hand

Registered: 04/03/00
Posts: 497
Last seen: 19 years, 5 months
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Re: How Many Generations Can You Grow Of Mycelium
#86377 - 06/20/00 02:36 AM (20 years, 8 months ago) |
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It varies from strain to strain. All you can do is try it and when you see a deterioration in the strain then it is time to go back to spores or clone another shroom------------------ Be sure to check out www.3mshrooms.homestead.com for your spore needs
-------------------- Corruptissima republicae, plurimae leges
Illegitumus non carborundum
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Workman
Psilocybe Microscopist


Registered: 03/01/01
Posts: 3,566
Loc: Washington, USA
Last seen: 2 years, 3 months
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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
#86379 - 06/21/00 01:22 PM (20 years, 8 months ago) |
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A good rule of thumb is 10 transfers or less without significant degeneration. Alteration of media between transfers helps maintain strain integrity. Some species, such as morels, tend to degenerate faster.------------------ The Spore Works Exotic Spore Supply sporeworks.com
-------------------- Research funded by the patrons of
The Spore Works Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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Anonymous
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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
#86380 - 06/21/00 06:40 PM (20 years, 8 months ago) |
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So in theory, a cultivator could use agar to grain....then back to agar (from the grain) indefinately???? Your are in fact changing the substraight!Just a thought
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WildLASER
Infinity In AGrain Of Salt

Registered: 04/02/00
Posts: 870
Last seen: 1 year, 2 months
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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
#86381 - 06/22/00 06:08 PM (20 years, 8 months ago) |
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I wouldn't say indefinately, it will help, but it won't keep it going 1000000's of times, I have no idea how less signifigant the degredation is, but I assume its not large enough to lead to indefinate continuation.
-------------------- ,'~Þ
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Anonymous
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Re: How Many Generations Can You Grow Of Mycelium [Re: 3Mshroom]
#86382 - 06/25/00 04:56 AM (20 years, 8 months ago) |
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I would also agree with Workman, I never use the first generation for inoculation I sector the ryzomorphic (ropey) sectors and transfer to a new agar medium. I have gotten up to 20 generations from B+ and only 10 from mazatapec. So I think it depends on the strain.
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