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Offlinedeathw
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newbie first injection/shelfing questions
    #8637136 - 07/15/08 10:18 AM (15 years, 8 months ago)

i started out using the pf tek method and i did everything as the method called for 2 verm, 1 water, 1 brf, mix em up nice and fluffy, and than i put into jars, left a little on top and filled rest with dry verm, sealed caps, and wraped with foil. than i steam sterilized for about a hour and a half, made a glove box with some shitty holes! ouch! shook up syringes and injected first few i wasnt getting the spores to run down the full side of the jar but it was injected and after the 3rd jar i got it right. after sterilizing the needle jar after jar, i switched to the next five and process repeated. medical gloves and sleves were worn.

after completion of injections i took the glovebox to the empty closet and has a nice high shelf about  6 feet up i opened the glove box and placed the 5 on the left and 5 on the right which was 5 Hawaiian and 5 Australian, the conditons in the closest at night 77-79F and during the day ranging from 80-85F also the inject points i didnt reseal with tape the 4 holes on each jar so the 4 holes arent covered anymore the holes are open as much as the syring made.

The only questions i have is that when i cleaned that closet i used the lysol wipes to wipe everything down and than used the spray to give it a once over and it still has that scent to it from the lysol does that matter at or or should i move the jars to another closet immediately?

Second should i keep the windows open or closed...? the closet isnt a full door its one of those sliding doors with the layers of wood one after another on a slant that air can get through.. should i seal up door to stop air or  just keep it the way i have them, ill post pictures as soon as i can as i will start a grow log in the proper section.

and himidity for the jars does that matter at all should i try and keep a certain level or just forget that process the jars have been sitting since yesterday at around 12:00pm so its been 1 full day already...

if anything you can help me with or advice to keep these jars safe from contams than please advice me as i would love to see atleast 1 cake work out well..would love all 10! im also looking to inject 10 more cakes tonight i might wait another day to see if antyhing at all shows on the cakes progress to see if the way i did everything worked ok and can remaster a couple things i did the first time.

sorry for the long message but help is greatly appreciated ESPECIALLY from you long time growers!!!!!!!!!!!!!!!

thank you!!!!!


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InvisibleLedd
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Re: newbie first injection/shelfing questions [Re: deathw]
    #8637145 - 07/15/08 10:25 AM (15 years, 8 months ago)

I think your just a little to worried. The only issue I spot is the day time temp might slow em' down a bit.

Did you punch the holes in your jar lids with the syringe? If so, don't lol.


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Offlinepsg1
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Re: newbie first injection/shelfing questions [Re: deathw]
    #8637148 - 07/15/08 10:27 AM (15 years, 8 months ago)

If everything goes well, are you going to be able to fruit 20 cakes at once? If not, hold off on the other 10 until the first set is fully colonized.

And don't think about this too much. The lysol won't hurt anything, you don't need to worry about humidity and fresh air exchange with colonizing jars. Your job now is to sit tight and wait.

Toss a thermometer up there with 'em if you like and make sure they're not getting far above 80F. Even if they are, the higher temps won't kill them, but will slow progress and favor possible contams.

Also, I'd remove any tape you've got on the holes still.


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Offlinedeathw
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Re: newbie first injection/shelfing questions [Re: Ledd]
    #8639758 - 07/15/08 08:34 PM (15 years, 8 months ago)

Quote:

Ledbonzo86 said:
I think your just a little to worried. The only issue I spot is the day time temp might slow em' down a bit.

Did you punch the holes in your jar lids with the syringe? If so, don't LOL.




No I took a small drill bit and drilled the 4 holes in the caps before I put the mixture and sterilized them. But I did put tape over the holes for sterilization along with foil but when I injected them I didn't recover the tape I left the holes alone whatever size the syringe made is the hole


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Offlinedeathw
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Re: newbie first injection/shelfing questions [Re: psg1]
    #8639823 - 07/15/08 08:44 PM (15 years, 8 months ago)

Quote:

psg1 said:[/b

Toss a thermometer up there with 'em if you like and make sure they're not getting far above 80F. Even if they are, the higher temps won't kill them, but will slow progress and favor possible contams.

Also, I'd remove any tape you've got on the holes still.




I have a thermomter sitting inbetween the 5 on the left and the 5 on the right, I'll sterilize some gloves with alcohol and remove the rest of the tape with the syringe holes for better gas exchange

This closet is so big I could fit 8 or more fc at once above ground of course

I think bc it's my first I wanna do thing right and your guys input is very important to me.


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InvisibleLedd
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Re: newbie first injection/shelfing questions [Re: deathw]
    #8639848 - 07/15/08 08:48 PM (15 years, 8 months ago)

You don't need sterile gloves. As long as you don't disturb the dry verm (tilting, shaking, abrupt jolts etc.) you will be fine. I have 12 jars going that I check more than once a day. Sometimes I wash my hands before I pick em' up, sometimes I don't. I have no contams and this is my first grow. But ya, remove the tape. Gas exchange (not FAE) is good.


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Offlinedeathw
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Re: newbie first injection/shelfing questions [Re: Ledd]
    #8640502 - 07/15/08 11:32 PM (15 years, 8 months ago)

I mean when I handled the dry vern moved a little but not like I'm shaking the thing just a first time worried grower ill just give my hands a wash with anti bacteria soap and rip off all the tape


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Offlinedeathw
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Re: newbie first as/shelfing questions [Re: deathw]
    #8640769 - 07/16/08 01:16 AM (15 years, 8 months ago)

Should I be packing my dry verm on top harder so it doesn't move much? And what about my mix should I be packing the top harder so when I inject it doesn't move the wet verm or is it going to make it move no matter what?


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OfflinewarGOD
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Re: newbie first as/shelfing questions [Re: deathw]
    #8641034 - 07/16/08 03:20 AM (15 years, 8 months ago)

good-luck


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Edited by warGOD (07/16/08 10:16 AM)

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OfflineSyMaNtiCX66
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Re: newbie first as/shelfing questions [Re: warGOD]
    #9355021 - 12/02/08 10:30 PM (15 years, 3 months ago)

hey i have a question or 2 why would my mycelium stop growing in some jars but not the rest? inoculated nov 20  78 to 82 degrees.. 2 jars are aLMOST done colonizing, just 3 have seemed to stop..

20 i may have dropped a minute drop of rubbing alcohol into the top dry layer of vermiculite.. will this kill my jar??? will alcohol fume effect them? 

thanks....

how do i posy my own questions on the shroomery?


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Just for A sEconD,, as i regAin foCus... aNd  everYthiNg arOund mE breatheS iN motiOn..with mY tiMe in a botTle,, a straNge vaCatIon,, her gigGles brEak throUgh anD suSpend aniMatIon......

SyMaNtIcx66...

Ghost Riderz!!!

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OfflineDamion5050
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Re: newbie first as/shelfing questions [Re: SyMaNtiCX66]
    #9355519 - 12/02/08 11:53 PM (15 years, 3 months ago)

Quote:

SyMaNtiCX66 said:
hey i have a question or 2 why would my mycelium stop growing in some jars but not the rest? inoculated nov 20  78 to 82 degrees.. 2 jars are aLMOST done colonizing, just 3 have seemed to stop..

20 i may have dropped a minute drop of rubbing alcohol into the top dry layer of vermiculite.. will this kill my jar??? will alcohol fume effect them? 

thanks....

how do i posy my own questions on the shroomery?





78-82 degrees is to hot some would say.. You have to remember that it produces heat so the inside of the jar is about 5-8 degrees hotter than the outside..  You want between 70-75 degrees...

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InvisibleOgre812
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Re: newbie first as/shelfing questions [Re: Damion5050]
    #9355621 - 12/03/08 12:12 AM (15 years, 3 months ago)

If I were you I would put the box on the floor where it should be a bit cooler.  The dry verm in your jars should be an alright contamination barrier, I don't even do BRF cakes but I put micro pore tape over my inoculation holes and dab them with touch of alcohol, to make sure the tape is sterile.  It should still allow for good gas exchange.

Quote:

hey i have a question or 2 why would my mycelium stop growing in some jars but not the rest? inoculated nov 20  78 to 82 degrees.. 2 jars are aLMOST done colonizing, just 3 have seemed to stop..





From what I know if some of the jars have halted growth it could be a sign of bacterial contamination.  As far as posting goes, when you go into a forum there is a button next to the forum description that says " POST".  One must be aware of his surroundings at all times young grasshopper.


Now as I leave you guys, I must have you realize that I am a noob at this hobby.  Take my advice as you will, but all my wisdom has been collected directly from this place, so I don't think it will be too far off.


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Those who would give up Essential Liberty to purchase a little Temporary Safety, deserve neither, and will lose both.      ~ Benjamin Franklin ~

"Let me tell you about a Porcupine's balls.  They're small, and they don't give a SHIT!"  ~Danny~

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Invisiblenw_shroomy
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Re: newbie first as/shelfing questions [Re: Ogre812]
    #9355655 - 12/03/08 12:19 AM (15 years, 3 months ago)

Quote:

If I were you I would put the box on the floor where it should be a bit cooler



I know some ppl do put there boxes on the floor but from What Ive read is that 90% of the contams are Down there.
I was thinking of putting my grow on the floor as I didnt have room and it was recommended not to.


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http://www.shroomery.org/forums/showflat.php/Number/7803673#7803673
I only grow edibles.Any info I give ONLY applies to gourmet mushrooms.

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InvisibleOgre812
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Re: newbie first as/shelfing questions [Re: nw_shroomy]
    #9355754 - 12/03/08 12:38 AM (15 years, 3 months ago)

With my limited amount of grows I have always kept my jars in a box on the floor.  I've only had one bout of contamination, and I believe that was due to the way I prepared my grains on that particular attempt.  With the added barrier of the micro pore tape, and not opening the box all the time, I think it will be just fine.


--------------------
Those who would give up Essential Liberty to purchase a little Temporary Safety, deserve neither, and will lose both.      ~ Benjamin Franklin ~

"Let me tell you about a Porcupine's balls.  They're small, and they don't give a SHIT!"  ~Danny~

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OfflineSyMaNtiCX66
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Re: newbie first as/shelfing questions [Re: Ogre812]
    #9355768 - 12/03/08 12:43 AM (15 years, 3 months ago)

THANKS!!! I NEED TO TURN THE HEAT PAD DOWN THEN. PLUS I HAD AIRTIGHT SEALS ON THE HOLES AT THE TOP. MABEY THE GAS EXCHANGE STUNTED THE SLOW ONE'S GROWTH... AS FOR THE HEALTHY ONES, THEY LOOK GREAT,,,, ILL POST PICS TOMORROW.. WILL GET MICRO PORE TAPE TO...

PS I HAVE THEM INSIDE THE BOX THE JARS CAME IN AND ALSO INSIDE THE TERRARIUM I AM GOING TO USE IN THE FUTURE.

THANKS FOR THE HELP EVERYONE!!!!


--------------------
Just for A sEconD,, as i regAin foCus... aNd  everYthiNg arOund mE breatheS iN motiOn..with mY tiMe in a botTle,, a straNge vaCatIon,, her gigGles brEak throUgh anD suSpend aniMatIon......

SyMaNtIcx66...

Ghost Riderz!!!

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InvisibleOgre812
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Re: newbie first as/shelfing questions [Re: SyMaNtiCX66]
    #9355813 - 12/03/08 12:55 AM (15 years, 3 months ago)

You most likely don't need that heat pad.  How cold would you say your closet gets down to?


--------------------
Those who would give up Essential Liberty to purchase a little Temporary Safety, deserve neither, and will lose both.      ~ Benjamin Franklin ~

"Let me tell you about a Porcupine's balls.  They're small, and they don't give a SHIT!"  ~Danny~

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OfflineSyMaNtiCX66
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Re: newbie first as/shelfing questions [Re: Ogre812]
    #9355828 - 12/03/08 01:00 AM (15 years, 3 months ago)

right now its in an old house that has minimal insulation so it's been getting down to 28 lately.. that perticular room has no window heater.. no one lives in it which makes it perfect for hiding... but the heating pad inside the terrarium keeps it nice. it will turn down to 70 i have tested it with a thermometer.


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Just for A sEconD,, as i regAin foCus... aNd  everYthiNg arOund mE breatheS iN motiOn..with mY tiMe in a botTle,, a straNge vaCatIon,, her gigGles brEak throUgh anD suSpend aniMatIon......

SyMaNtIcx66...

Ghost Riderz!!!

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InvisibleOgre812
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Re: newbie first as/shelfing questions [Re: SyMaNtiCX66]
    #9355871 - 12/03/08 01:13 AM (15 years, 3 months ago)

Damn!  Are you sure you haven't mistaken your refrigerator for you closet?  When I need some heat for the colonizing stage, I like to use the tub-in-tub tek.  I don't remember where I read it right now, but I read that a heating pad can be bad to use.


--------------------
Those who would give up Essential Liberty to purchase a little Temporary Safety, deserve neither, and will lose both.      ~ Benjamin Franklin ~

"Let me tell you about a Porcupine's balls.  They're small, and they don't give a SHIT!"  ~Danny~

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Offlinedeathw
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Re: newbie first as/shelfing questions [Re: Ogre812]
    #9356336 - 12/03/08 06:55 AM (15 years, 3 months ago)

Wow my old post comes back to life!! Haha, just bc the original post I made everything worked out great and fruited well

I can't stop having problems with my tit I swear temp is always good but I think shit just stops bc of to much co2 buildup, I hate the fucking winter!


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OfflineSyMaNtiCX66
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Re: newbie first as/shelfing questions [Re: deathw]
    #9360287 - 12/03/08 05:34 PM (15 years, 3 months ago)

HELP

i have a few jars colonizing well, some have stopped, some just look a lil funny..... input would be appreciated.

l=http://files.shroomeryurl=htt[p://files.shroomery.org/files/08-49/834744576-fungi_picx_005.jpg][/url]url=htt[p://files.shroomery.org/files/08-49/834744576-fungi_picx_005.jpg][/url]


sorry some of the pics are blurry


--------------------
Just for A sEconD,, as i regAin foCus... aNd  everYthiNg arOund mE breatheS iN motiOn..with mY tiMe in a botTle,, a straNge vaCatIon,, her gigGles brEak throUgh anD suSpend aniMatIon......

SyMaNtIcx66...

Ghost Riderz!!!

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