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Anonymous

Re: Culture no longer producing spores after third generation.
    #86254 - 06/15/00 01:17 AM (22 years, 3 months ago)

Contaminants, not the subtrate.. Common affliction of cubes and agaricus (button mushrooms)... double check your sterility and methods.


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Anonymous

Re: Culture no longer producing spores after third generation.
    #86256 - 06/15/00 01:20 AM (22 years, 3 months ago)

Contaminants, not the subtrate.. Common affliction of cubes and agaricus (button mushrooms)... double check your sterility and methods.


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Anonymous

Re: Culture no longer producing spores after third generation.
    #86257 - 06/15/00 01:57 AM (22 years, 3 months ago)

So your saying my mushrooms have contamination? What kind of contamination do I have that I can't see. The casing looks perfect, and there is no other color except the white of the mycelium - no cobweb mold either.


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InvisiblePrellgott
addict
Registered: 02/09/00
Posts: 383
Re: Culture no longer producing spores after third generation.
    #86258 - 06/15/00 12:00 PM (22 years, 3 months ago)

I have the same problem with a strain I got as mycelium syringe, it is nearly sterile, producees nearly no spores = has cream white gills.....

I've seen some sterlie shrooms accur with my cams (only jar that fruited out of 10(cam3))

and workman told me that it occurs from time to time.....

hope that helped



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i'm back


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InvisiblePrellgott
addict
Registered: 02/09/00
Posts: 383
Re: Culture no longer producing spores after third generation.
    #86259 - 06/15/00 12:00 PM (22 years, 3 months ago)

I have the same problem with a strain I got as mycelium syringe, it is nearly sterile, producees nearly no spores = has cream white gills.....

I've seen some sterlie shrooms accur with my cams (only jar that fruited out of 10(cam3))

and workman told me that it occurs from time to time.....

hope that helped



--------------------
i'm back


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InvisiblePrellgott
addict
Registered: 02/09/00
Posts: 383
Re: Culture no longer producing spores after third generation.
    #86260 - 06/15/00 12:01 PM (22 years, 3 months ago)

I have the same problem with a strain I got as mycelium syringe, it is nearly sterile, producees nearly no spores = has cream white gills.....

I've seen some sterle shrooms accur with my cams (only jar that fruited out of 10(cam3))

and workman told me that it occurs from time to time.....

hope that helped



--------------------
i'm back


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Anonymous

Re: Culture no longer producing spores after third generation.
    #86261 - 06/16/00 05:22 AM (22 years, 3 months ago)

So does anyone know the cause of sporeless mushrooms or is there no explanation except for maybe genetic degredation.


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Anonymous

Re: Culture no longer producing spores after third generation.
    #86262 - 06/16/00 11:28 AM (22 years, 3 months ago)

Its either Strain degenration, or as the learner suggested some kind of contam. To save your strain you must work with agar.

trade_omlet

------------------
How do you like your eggs?
scrambled? OMLET?



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InvisibleHippie3
mycotopiate
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Registered: 11/07/99
Posts: 3,090
Loc: mycotopia.net
Re: Culture no longer producing spores after third generation.
    #86263 - 06/16/00 03:30 PM (22 years, 3 months ago)

the brown streaks up the stem are definitly contams, bacteria.
but whether that caused sterility is another question,
which i do not know.

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OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 20 years, 5 months
Re: Culture no longer producing spores after third generation.
    #86264 - 06/21/00 12:28 AM (22 years, 3 months ago)

You don't need agar to save your strain...you can isolate strains from PF style jars by cutting out the best looking mycelium and putting it into sterilized malt-water solution. Once the malt turns a fuzzy white, you can innoculate multiple jars with then mycelium, which is genetically the same strain (provided you cut out only one strain). Keep in mind that the most rhizomorphic strain produces the most primordia, but isn't necessarily the best strain in terms of production...


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