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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Culture no longer producing spores after third generation.
    #86254 -

Contaminants, not the subtrate.. Common affliction of cubes and agaricus (button mushrooms)... double check your sterility and methods.

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Re: Culture no longer producing spores after third generation.
    #86256 -

Contaminants, not the subtrate.. Common affliction of cubes and agaricus (button mushrooms)... double check your sterility and methods.

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Re: Culture no longer producing spores after third generation.
    #86257 -

So your saying my mushrooms have contamination? What kind of contamination do I have that I can't see. The casing looks perfect, and there is no other color except the white of the mycelium - no cobweb mold either.

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Re: Culture no longer producing spores after third generation.
    #86258 -

I have the same problem with a strain I got as mycelium syringe, it is nearly sterile, producees nearly no spores = has cream white gills.....

I've seen some sterlie shrooms accur with my cams (only jar that fruited out of 10(cam3))

and workman told me that it occurs from time to time.....

hope that helped



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i'm back

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Re: Culture no longer producing spores after third generation.
    #86259 -

I have the same problem with a strain I got as mycelium syringe, it is nearly sterile, producees nearly no spores = has cream white gills.....

I've seen some sterlie shrooms accur with my cams (only jar that fruited out of 10(cam3))

and workman told me that it occurs from time to time.....

hope that helped



--------------------
i'm back

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Re: Culture no longer producing spores after third generation.
    #86260 -

I have the same problem with a strain I got as mycelium syringe, it is nearly sterile, producees nearly no spores = has cream white gills.....

I've seen some sterle shrooms accur with my cams (only jar that fruited out of 10(cam3))

and workman told me that it occurs from time to time.....

hope that helped



--------------------
i'm back

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Re: Culture no longer producing spores after third generation.
    #86261 -

So does anyone know the cause of sporeless mushrooms or is there no explanation except for maybe genetic degredation.

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Re: Culture no longer producing spores after third generation.
    #86262 -

Its either Strain degenration, or as the learner suggested some kind of contam. To save your strain you must work with agar.

trade_omlet

------------------
How do you like your eggs?
scrambled? OMLET?


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Re: Culture no longer producing spores after third generation.
    #86263 -

the brown streaks up the stem are definitly contams, bacteria.
but whether that caused sterility is another question,
which i do not know.

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Visit http://www.drooldonkey.org Global Psychedelic Community, Forums & Chat, Teks & Tips.



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Admin @ mycotopia.net
Mycotopia

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Re: Culture no longer producing spores after third generation.
    #86264 -

You don't need agar to save your strain...you can isolate strains from PF style jars by cutting out the best looking mycelium and putting it into sterilized malt-water solution. Once the malt turns a fuzzy white, you can innoculate multiple jars with then mycelium, which is genetically the same strain (provided you cut out only one strain). Keep in mind that the most rhizomorphic strain produces the most primordia, but isn't necessarily the best strain in terms of production...

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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