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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8679263 - 07/25/08 05:11 AM (16 years, 5 months ago) |
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Yeah, that glovebox will be better than nothing. It won't last long though.
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MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 7 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8679273 - 07/25/08 05:18 AM (16 years, 5 months ago) |
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Indeed thats what I meant. Lol. Thanks again.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8679275 - 07/25/08 05:20 AM (16 years, 5 months ago) |
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No worries, just making sure.;)
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plonkerism
Psychadelic Holiday Maker



Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8681895 - 07/25/08 09:09 PM (16 years, 5 months ago) |
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Ah ok, ill try to make as many as i can. I have 15 sterile syringes at the moment. I might make them up tomorrow with one print and keep them until it warms up a little. Can i keep the syringes for a month? Obviously in sterile, cool, dark area.
Still waiting on jars anyway. I cant find a place to buy them locally so have ordered some online. I have ordered some plastic pf jars from MycoPath that are PC-able etc: http://www.mycopath.com/index.php?main_page=product_info&cPath=4&products_id=39. Dont know if these are any good, i hear using glass is better than plastic but these were cheap and available.
For the glovebox ill replace the cardboard box with a similar sized plastic tub with lid, turn it upside down and so lid is on the bottom, open the lid to put materials etc inside. ?
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8681914 - 07/25/08 09:13 PM (16 years, 5 months ago) |
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Yes, the syringes will last for several months, no problem.
Don't bother buying jars, especially from overseas. If you really want the ones they use in America, there are a few places here that sell them. Drinking glasses with foil work fine for the PF tek though and any old jar works fine for grains.
A plastic tub is the way to go for a easy and sturdier glovebox. No need to turn it upside down though, although I'm sure it makes little difference.
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plonkerism
Psychadelic Holiday Maker



Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8681978 - 07/25/08 09:27 PM (16 years, 5 months ago) |
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Lol really? I feel quite silly... still ill try with these jars anyway, no point wasting them.
In regards to using drinking glasses, sealing them with just aluminium foil will be ok?
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8682124 - 07/25/08 10:07 PM (16 years, 5 months ago) |
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Yep, for the PF tek anyway. You can with grains too, but it's far riskier. Just use one layer of foil with four pin holes in it, secured with a rubberband and then place another two layers tightly on top.
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MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 7 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8682734 - 07/26/08 01:23 AM (16 years, 5 months ago) |
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Be sure to have a good layer of dry vermiculite on the top. Its in the tek, but some people decide to skip that part, and assume its ok. Its not. Its probably the most important step in preventing contaminates.
I would buy some jars. You can get 6-12 usually for like 7-10 dollars.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8682775 - 07/26/08 01:44 AM (16 years, 5 months ago) |
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Not here.;)
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MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 7 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8682865 - 07/26/08 02:25 AM (16 years, 5 months ago) |
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Damnit. I keep missing the whole Australia thing. Why are they so much? Are they hard to get? Try ordering some?
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8682878 - 07/26/08 02:33 AM (16 years, 5 months ago) |
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We're a giant island in the middle of nowhere. Shipping anything to us costs more, our population is one fifteeth of yours, so demand is considerably less and we have the hishest taxes in the world. All of those converge to make products expensive. As for those jars in particular, they aren't common at all - they're made in the US right? - so the few places they do sell them are very expensive, comparatively speaking.
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MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 7 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8682885 - 07/26/08 02:39 AM (16 years, 5 months ago) |
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I have no idea where they are made. I bought my 20 jars at once, and use quarts for my grains as of recently =]
Damn I didn't know all of that about Aus. I mean I knew its a big island, but I thought it had a larger population than that.
Anyway, what if I bought some jars for people over there and shipped them? Would it be that expensive to do? Like lets say I went out, and bought some...Then shipped them. Maybe I'm not aware of shipping things overseas. I haven't really done it...
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8683819 - 07/26/08 11:18 AM (16 years, 5 months ago) |
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Population = 20 million.
Go to the UPS site and see for yourself. I've had quite a few people offer to ship various things to me. Unfortunately, shipping often costs several time the price of the product, often more than $100.
It would be much cheaper to buy them from an Australian supplier who pays far less shipping on bulk quantities. In my opinion, it isn't really worth it for the most part. Jars are jars, especially if they're just for grains. It is nice to have them all of uniform shape and size, but not actually necessary.
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MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 7 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8683966 - 07/26/08 12:18 PM (16 years, 5 months ago) |
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Find someone who blows glass. Thats insane to pay that.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8685290 - 07/26/08 06:38 PM (16 years, 5 months ago) |
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I know.
Canning jars are not the only useful jar to use though. Any jar works fine at the end of the day.
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plonkerism
Psychadelic Holiday Maker



Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8691263 - 07/28/08 03:27 AM (16 years, 5 months ago) |
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OK, Stage 1 is complete! I am now the proud owner of 14 B+ syringes! Procedure i used is as follows:
1. Boiled water (tap water) for 30 minutes. 2. Open sterile syringes from packaging, attached needle also in separate sterile packaging. 3. Withdrew boiling water into syringes 4. Allowed to cool for approx 20hours 5. Constructed Glove box
After syringes cooled: 5. Sprayed room with Glen 20 liberally so surfaces were damp, allowed to dry for a few minutes before starting. 6. Disinfected Glove box with Glen20 and Isocol Isopropyl Rubbing Alcohol 7. Placed print, sterile packaged scalpel, glass (boiled and wiped down with isopropyl), and syringes into glovebox, close lid. 8. Squirted one syringes worth of sterile water into glass 9. Scraped entire print into glass 10. Ejected remaining syringes water into glass 11. Withdrew syringes one by one replacing needle cap after on each, trying to make sure no bubbles ended up in the syringe.
Hows that sound? Here are pics of my setup and how the syringes look afterward. The print was huge so the syringes have an obvious dark tint.
 
 

Completed Syringes:
 
Ahh, glad thats over! Next step BRF mix!
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MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 7 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8691324 - 07/28/08 05:15 AM (16 years, 5 months ago) |
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Good job. They should turn out fine.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8691617 - 07/28/08 09:00 AM (16 years, 5 months ago) |
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Quote:
MHbound said: Good job. They should turn out fine.
Hell Yea
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plonkerism
Psychadelic Holiday Maker



Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: Blutjager]
#8695751 - 07/29/08 02:04 AM (16 years, 5 months ago) |
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Thanks guys for your help so far!! Next is making the BRF cakes. I bought 10L of fairly fine grade verm today. Cost me $21. Didnt realise it would be so pricey!
Now im searching for BRF. CHecked my local IGA (aussie local supermakret) and they only had processed brown rice, or rice flour. Going to Woolworths now to see if they have it there.
I have some perfect drinking glasses to use. Im going to make 4 cakes from those and the other 10 when the jars i ordered arrive. Ill have to watch RRs videos again to refresh my memory. Its one syringe per jar right? And usually 1cc per hole, 2cc if you want faster colonisation time? That makes a max of 8cc per jar.. Isnt that wasting the other 2mL if you are using 1 'ringe per jar? This confuses me a bit. Im sure it will be cleared up when i watch the video again, but feel free to yell at me in the meantime
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themange
Senior Citizen



Registered: 06/11/06
Posts: 2,809
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8696090 - 07/29/08 06:05 AM (16 years, 5 months ago) |
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you dont need to put more than 1/2 cc per hole. when i do pftek i only make 2 injection sites and i put 1cc in each.
if you put 8cc's in one its going to oversaturate and cause bacterial contams.
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