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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: themange]
#8700784 - 07/30/08 02:51 AM (15 years, 7 months ago) |
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ok im going to go 4 holes 1/2 cc in each. Cheers for that!
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8700810 - 07/30/08 03:11 AM (15 years, 7 months ago) |
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For vermiculite, go on a hydroponics store. It's around $35 for 100L.
Try healthfood stores for brown rice flour. If you thought the vermiculite was pricey, you're going to be horrified at the price of BRF.
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8700844 - 07/30/08 03:42 AM (15 years, 7 months ago) |
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Oh forgot to metion i found some Organic BRF today from a healthfood store. 500g was approx $8! Dangit!
Im making up some cakes tonight so theyll be ready to inoculate tomorrow. Making 3 tonight as i only have 3 glasses that are the right size and shape. Ill upload some pics when im done..,
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8701114 - 07/30/08 07:25 AM (15 years, 7 months ago) |
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Just made up the jars. I used 1 1/2 cups verm, 3/4 cup of BRF, 3/4 cup water, and drinking glasses with straight sides which are a nice shape. Measured them to be just over a 1/2 pint when filled to the brim.
Mixed water first to wet verm then added BRF, just like in RR's video - wiped lip down, added dry layer of verm. Sealed with layer of aluminium foil with rubber band, placed 4 holes in this layer. Wrapped another 2 layers over the top. Boiled in large pot using a stove rack and a plate wrapped in foil to keep the jars off the bottom. Filled the pot with water to just underneath the plate, just beneath the bottom of the jars. Started timer for 90 mins after reaching full boil then reduced to low boil so still producing lots of steam. Ive left them in the pot now to cool, going to inoculate tomorrow night.
Im going to wait a while before doing the rest to see how this goes. I think its too cold here at the moment for a fast colonisation, but ill be trying to keep them warm in my room. I might buy a small aquarium heater, could be useful. Was planning to store the jars in the glovebox after i inoculate.
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8706105 - 07/31/08 04:49 AM (15 years, 7 months ago) |
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inoculated 3 cakes today. Tore the aluminium foil a bit while injecting. Covered with 2 layers of al foil. Didnt flame sterilise the needle as it didnt seem necessary (needle and syringe came pre-sterilised)... Hope thats ok..
Here are some pics of my jars after innoc. I have left them in my glove box for incubation. My main concern is i think i may have fucked up the BRF mix. I used 1.5cups verm to 3/4 cup water and 3/4 cup BRF. The resulting mixture seemed very floury. Im not sure if ive done this right, even though i followed instructions exactly.
Help maybe?! Do these look any good?
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8706311 - 07/31/08 07:28 AM (15 years, 7 months ago) |
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They look right to me. You don't want the mix to be particularly wet looking. Providing you have the dry verm layer, make sure you take the top layers of foil off so you only have the layer acting as the lid. It's normal for the foil to tear when using it instead of a lid. Make sure you flame the needle in between in each cake to prevent cross-contamination.
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rugergirl79
lεṡвïαṉ εχτɾøḋïṉ^
Registered: 03/27/08
Posts: 2,113
Loc: cardboard box
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8706373 - 07/31/08 08:14 AM (15 years, 7 months ago) |
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You are good to go..... let us know how it is coming along for you. kat
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The
Some Asshole
Registered: 07/09/08
Posts: 139
Last seen: 7 months, 8 days
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: rugergirl79]
#8706381 - 07/31/08 08:20 AM (15 years, 7 months ago) |
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those jars look a little to big, what size are they?
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8706393 - 07/31/08 08:25 AM (15 years, 7 months ago) |
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Quote:
plonkerism said: Measured them to be just over a 1/2 pint when filled to the brim.
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rugergirl79
lεṡвïαṉ εχτɾøḋïṉ^
Registered: 03/27/08
Posts: 2,113
Loc: cardboard box
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8706411 - 07/31/08 08:31 AM (15 years, 7 months ago) |
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I have used half pints, when i first started......took forever to colonize completely but eventually they did...lol
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8706424 - 07/31/08 08:37 AM (15 years, 7 months ago) |
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What are usually used? Aren't half pints the norm?
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rugergirl79
lεṡвïαṉ εχτɾøḋïṉ^
Registered: 03/27/08
Posts: 2,113
Loc: cardboard box
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8706607 - 07/31/08 09:59 AM (15 years, 7 months ago) |
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yah 1/2 pints are the bees knees...kat
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: rugergirl79]
#8706620 - 07/31/08 10:06 AM (15 years, 7 months ago) |
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Right. The imperial system does my head in, I don't understand why you don't all switch to the infinitely superior metric system.
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rugergirl79
lεṡвïαṉ εχτɾøḋïṉ^
Registered: 03/27/08
Posts: 2,113
Loc: cardboard box
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8706633 - 07/31/08 10:10 AM (15 years, 7 months ago) |
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Hey i have no problem with tha metrics>.....
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MHbound
Ballin Out At All Cost
Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 11 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: rugergirl79]
#8706747 - 07/31/08 10:48 AM (15 years, 7 months ago) |
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Bad idea not flaming the needle. Not smart.
I did it one time, and guess what??? Every jar that I didn't flame the needle for got contaminated, but every jar that I did flame it they were fine.
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: rugergirl79]
#8710624 - 08/01/08 02:18 AM (15 years, 7 months ago) |
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Chat. That sucks. I figure if these do contam, its only three jars and 1 syringe lost, no biggie. I figured that since the jars are already sterile, and i did this inside a glovebox that it would not allow much chance for contams to get in. The few seconds the needle was exposed to air while sucking up the spore water could have contam'd them though. I guess ill just have to wait and see
Tripsis: Yeah i guess its right, just looks different to others pics. So you say leave them to incubate with just the one layer of foil (with the holes in it) covering? Does not need any more protection? I had stored them with the al foil wrapped over the top covering the holes, but have now removed it, so holes are exposed. Is this what you mean?
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Edited by plonkerism (08/01/08 02:21 AM)
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8710626 - 08/01/08 02:19 AM (15 years, 7 months ago) |
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And yeah America needs to get the metric system. FFS i cant work like this! Cup this pint that. Too much... Litres and Millilitres are where its at!
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8710684 - 08/01/08 03:04 AM (15 years, 7 months ago) |
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Yeah mate, as long as you have the dry verm layer, that's all you need as a barrier against contaminants entering. You need to allow for sufficient gas exchange though, otherwise they'll stall.
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8722785 - 08/04/08 04:10 AM (15 years, 7 months ago) |
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Well had a look at the jars today and still no sign of germination. Its only been 4 days though and temps are not optimum, i still havent been able to buy that aquarium heater. It ranges from as low as 16c at night and hovers around 21-22C during the day. I keep a normal heater going in my room to try and keep temps up, but i turn it down a bit at night - otherwise its friggin hot in my room!
So i know this is going to hamper colonisation for the time being... Hopefully it doesnt stop it all together.. I really need to get that heater, but ive waxed all my cash this month and its still another 2 weeks till i get paid, noOOO!
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risndeath
Stranger
Registered: 07/25/08
Posts: 137
Last seen: 15 years, 1 month
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8722800 - 08/04/08 04:16 AM (15 years, 7 months ago) |
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I did the exact same thing you did, but i flame sterilized.
its been 11 days for me and no signs yet, hopefully you will have better results then I do
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