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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8611267 - 07/09/08 02:26 AM (15 years, 8 months ago) |
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Why not just make a spore syringe yourself?
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: chobumms]
#8611275 - 07/09/08 02:28 AM (15 years, 8 months ago) |
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Quote:
chobumms said: Dude, check this out, The first time I tried growing mushrooms I put some potting soil in foil 9X11's and cooked it in my barbecue pit. I took my first spore prints and scraped some on the soil, all three spore prints. After a week I had a small white growth thev size of half a dime, then it never got bigger. Then I did the PF tek with a PC that came with no rocker! on some Birdseed. Then I checked the incubator to find maggots in everything. Being a newbie is hard. The search function is your best friend.
Damn, not inspiring...
I totally expect to fail the first time, but im cool with that. A learning process it is, and one im looking forward to undertaking!
Quote:
tripsis said: Why not just make a spore syringe yourself?
That is something i havent researched and didnt know was possible from a print. Researching how to do it now. Do know any good methods?
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Edited by plonkerism (07/09/08 02:30 AM)
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MHbound
Ballin Out At All Cost
Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 11 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: wisp]
#8611286 - 07/09/08 02:31 AM (15 years, 8 months ago) |
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Like tripsis said...Make your own syringe, or use whole grains. If you use whole grains...This needs to be done in a very sterile environment though...You can simply scrape off some of the print into the jar. I have never done this, but you can use about 1/5-1/10th of the print.
You'll want to use some tyvek or polyfil with whole grains.
http://www.shroomery.org/8403/Psilocybers-Syringe-Tek
Thats how you make a syringe.
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Edited by MHbound (07/09/08 02:32 AM)
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BrandNewbie
Captain
Registered: 05/21/08
Posts: 2,932
Loc: U.S.A.
Last seen: 4 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8611306 - 07/09/08 02:34 AM (15 years, 8 months ago) |
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Good luck on your first grow mate! You might find some of this information useful:
http://www.shroomery.org/forums/showflat.php/Number/1910621#1910621
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
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gethyn
MR.STINK FINGER
Registered: 03/03/08
Posts: 758
Loc: first row at church
Last seen: 8 months, 28 days
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: BrandNewbie]
#8611323 - 07/09/08 02:39 AM (15 years, 8 months ago) |
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my Q tips made it in
-------------------- I RESEMBLE THE REST
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: BrandNewbie]
#8611492 - 07/09/08 03:28 AM (15 years, 8 months ago) |
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Quote:
BrandNewbie said: Good luck on your first grow mate! You might find some of this information useful:
http://www.shroomery.org/forums/showflat.php/Number/1910621#1910621
thanks mate
ok ive just ordered a box of sterile 18g 12cc syringes... but with 1in needle instead of 1.5. Im guessing this wont really matter as long as i get get it deep enough. Thats what she said ;P
Making a spore syringe doesnt seem difficult. One thing im not sure of is how sterile my environment is going to be. Im reading http://www.shroomery.org/8681/Captains-Oven-Tek. Hopefull that will work. Otherwise Ill be working in a kitchen which ill clean with good ol' Glen 20, and isopropyl. I have no hood or glovebox...
How sterile does this need to be? It seems that if even a little bit of 'dirty' air gets in somewhere, the whole process is down the sink. Many stories ive read on here seem to indicate that its not that fragile. Just how touchy is it?
I want to make as few infection points as possible since i dont have pc and im worried that its all gonna go balls up on me..
has anyone had exp with just boiling the jars, syringes etc?
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Edited by plonkerism (07/09/08 03:33 AM)
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BrandNewbie
Captain
Registered: 05/21/08
Posts: 2,932
Loc: U.S.A.
Last seen: 4 years, 9 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8611583 - 07/09/08 03:56 AM (15 years, 8 months ago) |
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Turn a "rubermaid" type big plastic bin upside down with just a small amount, (enough for your forearms to fit), of the bin hanging over the edge of an extremely clean table. Turn off your AC, fans, coolers, etc. Spray the inside of the bin, the table, the air, walls, floor and dog with Lysol or equivalent, (Glen20? I'm a Yank so I don't know what this is...). The idea behind the upside down bin, (you need to see through it so do not use an opaque bin), is that you are creating a "still air" box.
The problem is, it's like an operation at the hospital. The slightest microbe will cause an infection, (contamination). A microscopic pore/germ in your mix will "fight" the spores to be able to grow. The same environment that is perfect for spores to grow into mycelium, is perfect for thousands of other crap to grow. What your goal is, is to keep out ALL contaminates, (contaminates are EVERYTHING but spores), until the mycelium is strong enough to keep the contaminates from growing.
I know, it sucks.
You aren't going to get very far unless you read, read, read! I know this sounds harsh, but that doesn't make it any less true.
The fact is, (and I really hope you are successful), if you don't read the syringe making teks before you attempt to make your syringe, you will fail and all your money/time will have been wasted.
Please read up on this stuff first. You will be VERY glad you did. Not to mention, It'll keep you from staring at the mailbox all day!!!
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: BrandNewbie]
#8611611 - 07/09/08 04:09 AM (15 years, 8 months ago) |
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Quote:
BrandNewbie said: Turn a "rubermaid" type big plastic bin upside down with just a small amount, (enough for your forearms to fit), of the bin hanging over the edge of an extremely clean table. Turn off your AC, fans, coolers, etc. Spray the inside of the bin, the table, the air, walls, floor and dog with Lysol or equivalent, (Glen20? I'm a Yank so I don't know what this is...). The idea behind the upside down bin, (you need to see through it so do not use an opaque bin), is that you are creating a "still air" box.
Yes,like this >>
O and my sincere apologies to anyone that gets misled and thinks that is the best way to do things,the "Still air" upside down fish tank is still a great idea I just don't want anyone to think I would still be stupid enough to use polyfill in the holes in my jar lids covered with foil,I HATE that stuff and learned my lesson long ago
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: Blutjager]
#8611614 - 07/09/08 04:10 AM (15 years, 8 months ago) |
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O and sorry for the crappy picture as well,I took it with my old phone
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: BrandNewbie]
#8611639 - 07/09/08 04:24 AM (15 years, 8 months ago) |
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Im reading up as much as i can at the moment, trying to cram as much info into this head as possible . That is a good suggestion, will try it. Definately the easiest rather than making my own glovebox (which i know isnt really that hard, just more work). I like the oven tek also, but i have a large kitchen which has walls only 3 sides with one whole side open to living area which would be contaminant heaven. If i do your method, would a bathroom be a decent place or will that have bum particles everywhere? It would be a much smaller area to sterilise, and id be cleaning my house at the same time!
i have 50 syringes coming so plenty of practice and we are in the middle of winter here. By the time i get my act together it will be 20C to 28C spring days, that is a good temp i hear..
also will need some advice on fruiting chambers.. I know the info is here and will do my own research but to really get my head around it, its best just to ask someone who knows. Anyway ill get to that when/if it comes to it. Looks a fair way off from here!
edit: oo thanks for the pick. See stuff like that really helps a noob like me
Edited by plonkerism (07/09/08 04:26 AM)
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MHbound
Ballin Out At All Cost
Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 11 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8611649 - 07/09/08 04:28 AM (15 years, 8 months ago) |
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I do my work in a bathroom...Well I do my inoculating in bathrooms. I go in the smallest one, close the door, spray everything with lysol...Meaning the toilet paper holders, the toilet, the sink, the walls, everthing else, and then I go out shutting the door behind me. Give it 5-10 minutes to settle, and move in to do the work.
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8611662 - 07/09/08 04:39 AM (15 years, 8 months ago) |
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Put everything into the "Still air" box that will be needed and spray everything down with some bleach solution and than wait a few min,the rest of the room really does not matter all that much because nothing should be able to fall onto your work area but it wouldn't hurt to spray a bit of oust or Lysol.
Do NOT spray it in the glovebox and than flick a lighter.. even a few seconds seems like a long time when you are completely engulfed in flames and all you can smell is the hair burning off of your arms,by the way,in an enclosed area like a glove box its more akin to an explosion than a fire)
Rub an alcohol pad over your inoculation site,flame you needle just to be safe,cool it with a drop of spore solution dripping through the needle and than cover the inoculation hole with an alcohol soaked piece of micopore tape
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: Blutjager]
#8611681 - 07/09/08 04:58 AM (15 years, 8 months ago) |
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ok ive decided to make a glovebox now. Seems like good option, and its not that hard either. Ill have plenty of time to make one while i wait ..
im going to do both LC and make a few syringes. See which works better. Ill stick to one 'strain' (or at least one of the prints i got) for now and store the rest in ziplocks in cool place for next attempt.
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8611784 - 07/09/08 06:06 AM (15 years, 8 months ago) |
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im also interested in buying these. http://www.mushbox.com/substrate-jars.html P
Pre-packaged and pre-sterilised brf cakes..
I know the substrate is easy to obtain and make myself, but without a PC this seems like a good option for a noob. Also cuts out a process which can lead to contamination..
I wondering if they can be shipped to Aus. If they are sterilised, does that mean they can be imported or are they a big no no?
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MHbound
Ballin Out At All Cost
Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 11 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8611823 - 07/09/08 06:23 AM (15 years, 8 months ago) |
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I doubt it. Australia won't allow spore syringes, but allow spore prints. They will not allow cultivation supplies either.
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: MHbound]
#8611862 - 07/09/08 06:45 AM (15 years, 8 months ago) |
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Agreed. If found, they will not be let through.
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themange
Senior Citizen
Registered: 06/11/06
Posts: 2,809
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: BrandNewbie]
#8611873 - 07/09/08 06:55 AM (15 years, 8 months ago) |
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Quote:
BrandNewbie said: you are creating a "still air" box.
not a still airbox persay..since those are completely sealed. this would be more of a... clean-area-with-less-chance-of-any-nasties-falling-on-your-working-area-box
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Asric
Fueled By Satan
Registered: 06/18/07
Posts: 247
Last seen: 8 years, 7 months
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8612176 - 07/09/08 08:59 AM (15 years, 8 months ago) |
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Either way you are going to need to make a syringe from your print if you want to do cakes. I would suggest making a glove box from a clear tote to keep your work more sterile when making a spore syringe, or if you can go to your stove pull out the oven rack and run the oven as a poor mans flow hood. I have never tried scraping spores dirrectly into an LC but I imagine it would be more troublesome than shooting spores from a syringe into an LC and hoping for the best. Check out some of the syringe making teks on the site, you wont regret it. You can make LCs from cakes as you mentioned or you can make crazy amounts of LC off of fully colonized jars of grain.
EDIT: if you are going with BRF cakes you dont need to buy the expensive premade jars. BRF cakes can be steam sterilized. Check out the original PF tek on the cultivation part of the site.
-------------------- Looking to trade for: Wedges of: Golden Oyster, Pink Oyster, Shaggy Mane, Stropharia R. Prints of: Z strain, Treasure Coast, Exotics, Morels Have to trade: Wedges: Pearl Oyster, Wellington Oyster, Grey Oyster, Lion's Mane Prints: B+ Other stuff: Micro centrifuge tubes, Home made artisan bouillon in chicken and shellfish (lobster and shrimp), Homemade pecan smoked salt
Edited by Asric (07/09/08 09:01 AM)
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Monkeycyst
Stranger
Registered: 09/27/04
Posts: 237
Loc: Gimpsuit Cafe
Last seen: 11 years, 10 days
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: plonkerism]
#8612203 - 07/09/08 09:10 AM (15 years, 8 months ago) |
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You need to make a spore syringe or liquid culture.
-------------------- "Worry is preposterous. We don't know enough to worry."
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plonkerism
Psychadelic Holiday Maker
Registered: 06/27/08
Posts: 508
Loc: all up in your patch
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Re: So ive just ordered some spore prints... first time attempt at cultivating! [Re: Monkeycyst]
#8616038 - 07/10/08 02:19 AM (15 years, 8 months ago) |
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Yeah i know. Im happy with Psilocybers tek, going to do that AND the LC. oohh la di da! also found some subs today which ill be using the cardboard tek on, then ill be planting myc in every suitable substrate in sydney lol
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