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OfflineacenXA
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Prints and Microscope
    #859312 - 09/02/02 05:53 PM (14 years, 3 months ago)

any resources on where i can learn about spore prints and microscopic viewing?
How much does this ability narrow down identifying ?


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OfflineToxicManM
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Re: Prints and Microscope [Re: acenXA]
    #859432 - 09/02/02 06:51 PM (14 years, 3 months ago)

To learn how to do a spore print, try the first thread in this forum. If you're not sure which it is, try this link.

As far as using a microscope in mycology, there are entire textbooks on the topic. The book I use is How to Identify Mushrooms to Genus III: Microscopic Features by David Largent, David Johnson, and Roy Watling. It is published by Mad River Press with ISBN 0-916-422-09-7.


Spore prints are essential in the identification of a mushroom. The main families of mushrooms are partially defined based on the spore print color.

Microscopy is how the professionals identify mushrooms when they want to be sure. If you want a certain identification, you need to learn how to look for what they want you to see, and get a copy of the latest monograph for the genus of the mushroom you're identifying. On the other hand, you can do a pretty good job without using a microscope on the majority of mushrooms you'll be interested in. If you want to get into microscopy seriously, expect to spend significant time, effort, and money.


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Happy mushrooming!


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OfflineacenXA
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Re: Prints and Microscope [Re: ToxicMan]
    #859463 - 09/02/02 07:10 PM (14 years, 3 months ago)

thanks, i'll look into that book.
mostly i'm interested in the biology of fungus reproduction. i would like to be able to sorta track from spore begining to spore ending. it's the sorta thing i would just like to add to my general body of knowledge.
any tips on using the microscope? like adding color or introducing a liquid medium?


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Anonymous

Re: Prints and Microscope [Re: ToxicMan]
    #859479 - 09/02/02 07:17 PM (14 years, 3 months ago)

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Offlinezeronio
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Re: Prints and Microscope [Re: ToxicMan]
    #860509 - 09/03/02 02:49 AM (14 years, 3 months ago)

I've been thinking to get a good microscope for a long time, but those who seem good (oil immersion) are very expensive. What kind of microscope do you have and at what magnification is enough for viewing spores?
Can you view also hyphae, clamp connections, cystidia, etc.?


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OfflineToxicManM
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Re: Prints and Microscope [Re: zeronio]
    #860710 - 09/03/02 05:50 AM (14 years, 3 months ago)

I got an inexpensive student microscope from the local science supply (about $250). You can see spores and other structures reasonably well at 400x which isn't high enough to require oil immersion. The main things I use 1000x (oil immersion) for are looking at spore ornamentation and photography. The hyphae, clamp connections, and cystidia are visible at 400x.


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Anonymous

Re: Prints and Microscope [Re: ToxicMan]
    #861334 - 09/03/02 11:36 AM (14 years, 3 months ago)

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Re: Prints and Microscope [Re: acenXA]
    #861357 - 09/03/02 11:48 AM (14 years, 3 months ago)

I'm not sure what you mean by "spore beginning" or "spore ending". As far as liquid media, I use water about 80% of the time and Melzer's Reagent the other 20%. As far as adding color, the book lists the stains that are most commonly used. Melzer's is the one I use by far the most. I also use Methylene Blue (cheap, easy to get, one of the best stains out there), an eosin (Rose Bengal, but most will do), and a few miscellaneous stains that were available. Thus far I'm mostly experimenting with the stains to see what features each tends to bring out. Melzer's is still the best one on the list, because it only stains amyloid within the cells, and only adds a brownish color (at most) to any other features. They are fun to play with for photography - ooooo, look at the pretty colors!


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Anonymous

Re: Prints and Microscope [Re: ToxicMan]
    #861371 - 09/03/02 11:57 AM (14 years, 3 months ago)

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OfflineacenXA
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Re: Prints and Microscope [Re: ]
    #862315 - 09/03/02 08:23 PM (14 years, 3 months ago)

yea thats exactly what i meant whole life cycle. and thanks TM, i've got plenty to go and keep me busy. only one more question which is do you print right onto glass? becuase i tried to transfer from paper to the glass and it didnt turn out that great becuase i ended up with globs of spores whicch i couldnt seperate. mad for poor viewing.


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Anonymous

Re: Prints and Microscope [Re: acenXA]
    #862319 - 09/03/02 08:28 PM (14 years, 3 months ago)

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OfflineacenXA
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Re: Prints and Microscope [Re: ]
    #862335 - 09/03/02 08:43 PM (14 years, 3 months ago)

MM i meant for microscopic viewing. how to get the spores to the microscope glass.


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Anonymous

Re: Prints and Microscope [Re: acenXA]
    #862353 - 09/03/02 09:00 PM (14 years, 3 months ago)

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OfflineToxicManM
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Re: Prints and Microscope [Re: acenXA]
    #862829 - 09/04/02 05:37 AM (14 years, 3 months ago)

No, I don't do the spore print right onto the glass. There would be so many spores that you could hardly see them. I do the print on paper, then use a dissecting needle to transfer a tiny amount to the slide. If you can see a tiny dot of spore material you have plenty. Then add a drop of water and a cover slip and look at spores.


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Happy mushrooming!


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Amazon Shop for: Microscope

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