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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
First timer mistake - do you think my PF-TEK jars still have a chance?
    #8573297 - 06/28/08 02:04 AM (16 years, 6 months ago)

Hi all,

So I just inoculated my first batch of PF-TEK jars for my first grow. When opening the aluminum foil I realized that my syringes shipped with very thick needles that wouldn't fit through the holes I had made pre-sterilization in the lid. Basically I had to open the jars to inoculate one by one...

I have pretty much sprayed an entire bottle of Lysol into the room throughout the day and a few minutes before starting. I also sanitized the needle with alcohol and flame after every jar and wiped down the surfaces with alcohol multiple times throughout inoculation as well as spraying the still air with Lysol again midway through the jars.

Each jar was exposed to ambient air for ~20 seconds for the first few and as little as ~10 seconds in the later ones when I got the hang of it. I was also wearing a face mask, clean clothes, and had covered my hair for the process.

So my question is, did I completely F up, or just slightly? Do they still have a chance?

Ironic how my attempts to be super sanitized ended up completely the opposite way...

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Offlinedjblackout
supreme scratch masta


Registered: 05/03/08
Posts: 1,870
Last seen: 14 years, 4 months
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: bahaiDOTorg]
    #8573309 - 06/28/08 02:14 AM (16 years, 6 months ago)

i dont understand why you didnt put something in the holes to widen them?  like a tiny screwdriver..


--------------------
I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You

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InvisibleHoleSnype
I love me some me.
Male User Gallery


Registered: 04/27/06
Posts: 4,315
Loc: DF DUBS
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: djblackout]
    #8573313 - 06/28/08 02:16 AM (16 years, 6 months ago)

Quote:

djblackout said:
i dont understand why you didnt put something in the holes to widen them?  like a tiny screwdriver..




I was thinking the exact same thing.

It is really hard to answer his question. Only time will tell. Opening jars is the worst thing you can do.


--------------------
"I'm sofa king we todd did." ~ Rick James

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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: djblackout]
    #8573315 - 06/28/08 02:17 AM (16 years, 6 months ago)

You are right. I should have. I may have been in a slight state of panic cuz it was my first time and went much more intensely for me than when you pros do it I am sure.

I somehow figured a few seconds of exposure would be better than messing with the holes contamination wise. But I guess I was wrong?

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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: HoleSnype]
    #8573319 - 06/28/08 02:21 AM (16 years, 6 months ago)

Quote:

Opening jars is the worst thing you can do.




:frown:

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Offlinejadels215


Registered: 06/20/08
Posts: 235
Last seen: 1 year, 10 months
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: bahaiDOTorg]
    #8573344 - 06/28/08 02:35 AM (16 years, 6 months ago)

doesnt the layer of vermiculite act as a barrier between air borne contaminants?

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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: jadels215]
    #8573375 - 06/28/08 02:52 AM (16 years, 6 months ago)

Quote:

jadels215 said:
doesnt the layer of vermiculite act as a barrier between air borne contaminants?




I can only hope:

http://www.shroomery.org/forums/showflat.php/Number/5296250#Post5296250

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Offlinethepaintingaccid
Stranger


Registered: 03/27/08
Posts: 251
Last seen: 14 years, 2 months
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: bahaiDOTorg]
    #8573418 - 06/28/08 03:25 AM (16 years, 6 months ago)

yah the verm barrier will help. best thing is just to ride them out and see how they do. You flame the needle?

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Offlinepsg1
viewer
 User Gallery

Registered: 02/12/06
Posts: 150
Last seen: 12 years, 3 months
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: thepaintingaccid]
    #8573553 - 06/28/08 06:59 AM (16 years, 6 months ago)

So you dropped the solution onto the dry verm or injected through it? Those spores will have a tough time germinating in dry verm...


--------------------
.

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Offlinedjblackout
supreme scratch masta


Registered: 05/03/08
Posts: 1,870
Last seen: 14 years, 4 months
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: psg1]
    #8574755 - 06/28/08 03:56 PM (16 years, 6 months ago)

well atleast youll know what not to do next time


--------------------
I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You

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OfflineMarioTrip
since84
Male


Registered: 10/21/07
Posts: 2,207
Loc: 69 w/ you
Last seen: 16 years, 3 months
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: djblackout]
    #8574766 - 06/28/08 03:59 PM (16 years, 6 months ago)

Better luck next time but some of them may pull through :thumbup:


--------------------
I'm Livin' It, distribute it
The streets are inhibited
By cut throat niggas just like me
that out there getting dividends

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Offlinehoodbran
Dosser
Male User Gallery


Registered: 06/01/08
Posts: 1,570
Loc: Phloston Paradise
Last seen: 9 months, 8 days
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: MarioTrip]
    #8574850 - 06/28/08 04:31 PM (16 years, 6 months ago)

fingers crossed 4 u, keep us posted!


--------------------
Not all drugs are good, Some are great.




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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: thepaintingaccid]
    #8575041 - 06/28/08 05:35 PM (16 years, 6 months ago)

Quote:

thepaintingaccid said:
yah the verm barrier will help. best thing is just to ride them out and see how they do. You flame the needle?




Yes, between every jar I used alcohol on the metallic and plastic part of the needle followed by flame.

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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: psg1]
    #8575044 - 06/28/08 05:36 PM (16 years, 6 months ago)

Quote:

psg1 said:
So you dropped the solution onto the dry verm or injected through it? Those spores will have a tough time germinating in dry verm...





I made sure the needle was well into the wet layer before injecting.

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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: bahaiDOTorg]
    #8575045 - 06/28/08 05:38 PM (16 years, 6 months ago)

Thanks for the replies! I will post after a few days. I will probably need you guys' help in deciding whether any grow I get is healthy or contaminated.

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Offlinedavidcoperfield
magic!
Male


Registered: 05/04/08
Posts: 244
Loc: ohio
Last seen: 9 years, 9 months
Re: First timer mistake - do you think my PF-TEK jars still have a chance? [Re: bahaiDOTorg]
    #8575047 - 06/28/08 05:39 PM (16 years, 6 months ago)

they will be fine. I did that once years ago... big rookie mistake, as long as you flame the needle and make sure your environment is sterile you should be fine. :wink:


--------------------
If I'm free, it's because I'm always running.
Jimi Hendrix

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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Fruiting Chamber Questions [Re: davidcoperfield]
    #8576229 - 06/29/08 01:45 AM (16 years, 6 months ago)

Hello shroomers and thank you again for your previous insights.

While waiting for my jars to colonize (if at all), I am building my FC and have a couple of questions on what I have researched. I will be fruiting PF-TEK cakes directly in the FC (ie not casing):

1. My hydrometer shows a constant 95 % humidity in the chamber with my current setup using an amphibian humidifier that works simply by blowing fresh air into water first and then feeding it to the FC. Do you think I will benefit from using perlite to reach %100 humidity?

2. My goal is to not have to fan during the day. I have a powerful aquarium air-pump (for 100 gallon water tanks) with two air outlets. One outlet is pumping fresh air into a water chamber and subsequently into the FC. The other one will be connected to a long two-feet aquarium "bubbler" that will basically be sitting on the bottom of the FC. Non of the incoming air flow will be hitting any cakes directly. Can I rely on this large amount of incoming air along with air escaping from the lid for proper FAE without "fanning" daily? If not, would this be achieved with a few/lots of holes on the FC walls?

From what I can tell, the lid is very loose fitting and air can easily escape out from the top and the pump inputs a significant amount of air into the chamber from bottom that I feel I should be more than OK not "fanning" (by hand) daily; But then again I don't have the expertise, which is why I am asking for your.

3. If you were to pick a direction for air-flow, what would be best? (ie incoming fresh air into bottom of FC and out of the top or vice versa etc).

Basically my situation is that if I "need" to open my chamber to fan daily, my cakes will suffer, because I am gone for up to 20 hours at a time. So I am trying to make it as autonomous as possible FAE-wise.

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OfflineBallarat_Bandit
Techno-Drugs
Male

Registered: 03/28/08
Posts: 51
Last seen: 16 years, 20 days
Re: Fruiting Chamber Questions [Re: bahaiDOTorg]
    #8576244 - 06/29/08 01:56 AM (16 years, 6 months ago)

Just to throw my two cents, I know someone who did practically the same thing his first time, opening each jar. If you are as careful as you say, and only opened them for a few seconds each you should be fine. Success rate for my friend was about 50%, and he did alot worse than you did. No worries.

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InvisiblebahaiDOTorg
Wonderer


Registered: 06/25/08
Posts: 44
Re: Fruiting Chamber Questions [Re: Ballarat_Bandit]
    #8576388 - 06/29/08 03:32 AM (16 years, 6 months ago)

Quote:

Ballarat_Bandit said:
Just to throw my two cents, I know someone who did practically the same thing his first time, opening each jar. If you are as careful as you say, and only opened them for a few seconds each you should be fine. Success rate for my friend was about 50%, and he did alot worse than you did. No worries.




Thanks for posting this. Gives me hope. Anyone have any advice on questions two posts above?

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Offlinephoreverphishin
Stranger


Registered: 05/01/08
Posts: 267
Last seen: 15 years, 7 months
Re: Fruiting Chamber Questions [Re: bahaiDOTorg]
    #8576419 - 06/29/08 03:55 AM (16 years, 6 months ago)

If your ever in a similar situation again post on here people will help believe me.

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