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OfflineBrandNewbie
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Re: omgot all my stuff ready... final prep questions everybody! [Re: beengonetoolong]
    #8568093 - 06/26/08 06:17 PM (16 years, 6 months ago)

Quote:

beengonetoolong said:
Quote:

BrandNewbie said:
Quote:

wishywashy said:
i PC'd them for about an hour with 5 1/2 pint jars around 4:30 today. it's now 7:30. when's good to innoc? i wanna do it tonite :laugh:




I've always read to wait overnight to inoculate your PC'd jars. They can feel cool enough, but still be too hot in the middle.  It would suck to be so close to success, only to accidentally cook your spores because you didn't follow instructions!

I just plan on a tomorrow inoculation if I PC today, that way I'm sure my jars are cool enough.  :rockon:




Well. I beg to differ, but once the glass of the jars are cool, usually 4-6 hours in my experience(9-10 years.. no im not an expert, just some experience)... they are just fine to inoculate.    So if you "really" want to save 12 hours by innoculating 4-6 hours after pcooking and they have cooled.  I see no problem. 

Nothing wrong with waiting, either. :smile:





Please forgive my ignorance. I've read that you should wait, over and over. In fact I waited on my jars for that very reason. However, I (just this minute actually, that's why I'm back at this post) just found out from RR that my previous post is "bs". (I'm thinking he means bullshit here). Again, sorry for the misinformation. I got it from a site called The Shroomery.

P.S. You're an expert compared to me. I swear I was just trying to pass on what I've "learned".


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Answer: Because they're ugly and they stink.

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Offlinebeengonetoolong
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Re: omgot all my stuff ready... final prep questions everybody! [Re: BrandNewbie]
    #8568121 - 06/26/08 06:24 PM (16 years, 6 months ago)

Either way is fine.. just saying.. no need to wait for 24 hours or anything crazy like that.. but its fine either way

:smile:


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Offlinebeengonetoolong
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8568123 - 06/26/08 06:25 PM (16 years, 6 months ago)

Quote:

wishywashy said:
i could just poke my finger in the substrate to see if the middle's hot right?




No.


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Is there life after death? Trespass and find out.

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OfflineBrandNewbie
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8568146 - 06/26/08 06:31 PM (16 years, 6 months ago)

Quote:

wishywashy said:
i could just poke my finger in the substrate to see if the middle's hot right?




Can you say "contamination"?

I was retracting what I said out of ignorance earlier. Just listen to Beengonetoolong. I gave you shit info. (Swear it wasn't on purpose!)


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Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.

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InvisibleHoleSnype
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Re: omgot all my stuff ready... final prep questions everybody! [Re: BrandNewbie]
    #8568194 - 06/26/08 06:41 PM (16 years, 6 months ago)

If you leave the lid on your PC, like you should, it and the jars won't be cool enough to inoculate in 4 hours. You don't want to open it until it is fully cooled down to room temp.

If your jars cool off rapidly, it can cause the verm layer to get moist, and that is not good.


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"I'm sofa king we todd did." ~ Rick James

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Offlinewishywashy
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Re: omgot all my stuff ready... final prep questions everybody! [Re: HoleSnype]
    #8568939 - 06/26/08 09:42 PM (16 years, 6 months ago)

ok all in all i've given the jars 7 or so hours to cool off. couldn't wait any longer i wanted to do this tonight!. here's my procedure, and do critique as i'm gonna get good at this shit one day:

4:30pm after done with the PC, i took off the pressure regulator, it appeared to have rapidly depressurized, but i didn't put it in the fridge or anything.

couple times between, i take off the cover after depressurizing to see if jars cracked. none did:)

11:30pm i prepare to inoculate! jars smell funny, but first time for everything. i open the PC, jars still have tin foil on top. wearing rubber gloves, i put down some cotton balls for absorbing the excess spore fluid. fumbling with the damn syringe, i now figured out how to use it. i heated the syringe red hot for 2-3 out of 5 jars... i know it was minutes ago but i don't remmeber the number :rolleyes: . am i supposed to red-hot the needle after every jar? or even every inoc site for each jar? dunno, fuck it.

after red-hotting, i squirt out some fluid so it doesn't sound as it's steaming when it comes out, on to the cotton balls. wearing a shirt to cover the breath from my face, i take off the foil from each jar, squirting that yummy b+spore into each jar. i only heated the needle for 2 out of 5 jars, hope it doesn't mold me in the ass :confused: .

then, i put the foil back on each jar, wrap tight, and put it back in my pc, lock it up and put it away! i'll check back on it every few days to see how it's going.

OBSERVATIONS:
red hot needle? more like orange, but good enough i hope. criticize me plzzzzzz

one inoc site brought up a piece of verm into the needle, that's when i reheated the bastard. it's fine i hope? lolol

there is little to no air flow in the room i'm in, just as instructed. i'm pretty sure it went well, but time will tell, hopefully you guys will too!

wishwash


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life rolls on

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InvisibleHoleSnype
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8568970 - 06/26/08 09:50 PM (16 years, 6 months ago)

Sterilize the needle between jars.


Take the foil off and take them out of the PC. Put them on a shelf at room temp.

The foil can and will cause problems.


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"I'm sofa king we todd did." ~ Rick James

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Offlinewishywashy
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8569012 - 06/26/08 10:01 PM (16 years, 6 months ago)

gotcha... any need to seal the inoc points with tape or anything?


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InvisibleHoleSnype
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8569023 - 06/26/08 10:04 PM (16 years, 6 months ago)

As long as you have a verm barrier you are ok. Hopefully it's fine grade verm on top.

Jars need gas exchange.


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Offlinewishywashy
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8569033 - 06/26/08 10:06 PM (16 years, 6 months ago)

ferti-lome? thats the brand i got a 10qt bagthere's some big chunks strewn about, but most pieces are fine


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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8569041 - 06/26/08 10:08 PM (16 years, 6 months ago)

Just don't flip it. Keep it straight up so you don't disrupt the verm barrier.


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Offlinewishywashy
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8569073 - 06/26/08 10:19 PM (16 years, 6 months ago)

k thxxx. check back in the next few days i may get some pics of ma jars/contams.
i'll keep it posted! still got like 6 syringes left i should get more jars :tongue:


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Offlinewishywashy
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8570534 - 06/27/08 10:17 AM (16 years, 6 months ago)

day 1 of incubation: 12:16 pm, no signs of life yet, perhaps a speck of white splotchy. now, they have to be absolutely in darkness and at room temp. is a minute or so of light going to affect? i had to move them to a darker place today so they were out in the open for a bit.


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Edited by wishywashy (06/27/08 10:19 AM)

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OfflineMHbound
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8570671 - 06/27/08 11:16 AM (16 years, 6 months ago)

No a minute isn't going to hurt them. Some people on these boards don't keep them in complete dark as you should. It can affect the time. After about 3-5 days I check mine, and then after I see growth I check them everyday to make sure everything is going as it should. No problems there IMO.


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Offlinehxcjohn
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Re: omgot all my stuff ready... final prep questions everybody! [Re: MHbound]
    #8570911 - 06/27/08 12:40 PM (16 years, 6 months ago)

BrandNewbie: I don't think you advice was BS at all, just on the safe side. Is it going to mess anything up waiting a few more hours? No. So BrandNewbie, you keep on keeping on.
wishywashy: It seems you haven't read much, or even watched a video. You ask alot of questions you can answer yourself with a little studying. Good job on getting your first jars going. Try not to check them every 5 minutes. Oh also make some LC so those syringes go further.


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Disclaimer: I do not buy, sell, cultivate, manufacture, or use any illegal substance. Any postings I make on this forum are purely for entertainment purposes. Any pictures that I may post have been created by artificial means from images gathered from the internet and other sources. Any statements made should not be considered truthful.

Edited by hxcjohn (06/27/08 12:53 PM)

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Offlinewishywashy
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8583306 - 07/01/08 08:40 AM (16 years, 6 months ago)

been 4 days since inoc.... wondering if i did something wrong, no mycelium yet. when i made the BRF cakes, i just mixed in the flour with dry verm at the same time. RoadKill did it differently but that wouldn't fuck the whole process, rite?


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OfflineMHbound
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8584041 - 07/01/08 01:14 PM (16 years, 6 months ago)

As long as you sterilized no. 3-5 days is how long it generally takes to see growth. Just sit back. It could take a week.


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Offlinenumonkei
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Re: omgot all my stuff ready... final prep questions everybody! [Re: MHbound]
    #8584074 - 07/01/08 01:24 PM (16 years, 6 months ago)

You're going to need to develop some patience, guy. Sometimes it can take a week or two to show growth, depending on the particular strain and many other environmental conditions.

You did NOT seal the holes, correct? You don't want to do that, the verm layer will should keep out any contams that may work through the holes.

Gotta be patient buddy.




~Monk

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Offlinewishywashy
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Re: omgot all my stuff ready... final prep questions everybody! [Re: numonkei]
    #8587628 - 07/02/08 12:16 PM (16 years, 6 months ago)

day 4 after inoculation:

w00t i see nice splotches on each of my 5 jars <3

i'm gonna check the contam forums cuz i'm curious

so i see this process taking at most 3 weeks, but is it dependent on how much sporejuice you use? also, i want to wait till all of the jars are colonizing so i can dunk n roll them all at once.


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OfflineMHbound
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Re: omgot all my stuff ready... final prep questions everybody! [Re: wishywashy]
    #8587832 - 07/02/08 01:26 PM (16 years, 6 months ago)

Why don't you post them here, and we will tell you. You could also change your thread to be your new grow log. I would like to watch your stuff come along. :thumbup:


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