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Amazon Shop for: Agar, Malt Extract, Potato Dextrose

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Offlinepaddo
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question about agar
    #854395 - 08/31/02 06:09 AM (14 years, 3 months ago)

hi,
i'm gonna make some agar now,

but i have one question,

i'm goin to make Malt extract agar, so:

20 grams malt
2 grams yeast
20 grams agar

would it be good or bad to put some dextrose into to the mixture?

and if it is good , how much should i use?


thanks,
paddo.


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InvisibleTripsAreForKids
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Re: question about agar [Re: paddo]
    #854458 - 08/31/02 07:29 AM (14 years, 3 months ago)

That would be fine without the dextrose. IMHO its a waste


--------------------
Everything posted above was out of fun, none should be taken serious. I am currently under the influence so take that into consideration.


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InvisibleCLuB99
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Re: question about agar [Re: paddo]
    #854523 - 08/31/02 08:34 AM (14 years, 3 months ago)

is not good nor bad...just isn't necessary
if i can suggest you don't stuck with malt agar...in my experience isn't all that good..
PDA works way better, but my fav is OMYA, oatmeal malt yeast agar...works like a charm!


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Offlinepaddo
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Re: question about agar [Re: CLuB99]
    #854540 - 08/31/02 08:47 AM (14 years, 3 months ago)

Hi club,

Thanks for the tips!

This is my first time experimenting with agar.
Is malt extract agar realy that bad?
I hope it turns out ok....


what are the benefits of using PDA compared to MEA?

Thanks,
Paddo


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Offlineaural
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Re: question about agar [Re: paddo]
    #854571 - 08/31/02 09:16 AM (14 years, 3 months ago)

MYA is fine.Don't worry-it'll work great.Next time try a different recipe if you feel like it.


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InvisibleCLuB99
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Re: question about agar [Re: paddo]
    #854632 - 08/31/02 10:12 AM (14 years, 3 months ago)

with mya or even mypa (with peptone added) i was never able to get rizomorphic growth, even from a known culture...
the same culture thrives on pda or omya, but could be just my malt extract wich isn't all that good...


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Offlinepaddo
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Re: question about agar [Re: aural]
    #854641 - 08/31/02 10:18 AM (14 years, 3 months ago)

ok :smile:


I know that it is good to change the agar formula from time to time when one is cloning.
Because of the ability of the mushroom mycelium to produce digestive enzymes.
when changing the media , the strain's enzyme system remains broadly based and the mycelium is better suited for survival.

But I just inoculated the agar with a multispore mazatapec.

When everything is turning out ok im goin to transfer a rhizo agar wedge (sectoring) to a new agar plate also using the same agar formula.


Paddo 


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InvisibleCLuB99
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Re: question about agar [Re: paddo]
    #855021 - 08/31/02 02:41 PM (14 years, 3 months ago)

so looks like your hood is doing well :smile:


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Offlinepaddo
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Re: question about agar [Re: CLuB99]
    #855028 - 08/31/02 02:45 PM (14 years, 3 months ago)

hehehe :wink:

Yep its working perfectly, just finnished it today..

Gonna post some pics tomorrow of the flowhood :wink:



Paddo 


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InvisibleDarkmoon
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Re: question about agar [Re: paddo]
    #858267 - 09/02/02 06:02 AM (14 years, 3 months ago)

did you get any pics yet of your flowhood??  I would love to see them when you post them so let us know!!  :wink: 


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Offlinepaddo
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Re: question about agar [Re: Darkmoon]
    #858292 - 09/02/02 06:41 AM (14 years, 3 months ago)



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Amazon Shop for: Agar, Malt Extract, Potato Dextrose

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