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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Invisiblelegallyhomeless
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Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
Re: DON'T alcohol after heating up syringe needle? [Re: anarchOi]
    #8540898 - 06/19/08 01:50 PM (15 years, 7 months ago)

Quote:

anarchOi said:
homless you kind of remind me of the kid who said "DON'T STOMP ON ANTS! HOW WOULD YOU LIKE TO GET STOMPED ON IF YOU WERE AN ANT?!"




WHATS THAT SUPPOSE TO MEAN?!?! I LOVE ANTS!!! :rant: :rofl:


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OfflineNeobean
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Registered: 10/07/01
Posts: 975
Loc: Canada
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Re: DON'T alcohol after heating up syringe needle? [Re: legallyhomeless]
    #8540916 - 06/19/08 01:56 PM (15 years, 7 months ago)

Quote:

legallyhomeless said:
Quote:

anarchOi said:
homless you kind of remind me of the kid who said "DON'T STOMP ON ANTS! HOW WOULD YOU LIKE TO GET STOMPED ON IF YOU WERE AN ANT?!"




WHATS THAT SUPPOSE TO MEAN?!?! I LOVE ANTS!!! :rant: :rofl:




Its a shroomerite's way of flirting with you :P


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Offlinepraga
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Registered: 04/19/08
Posts: 32
Last seen: 15 years, 3 months
Re: DON'T alcohol after heating up syringe needle? [Re: Neobean]
    #8541140 - 06/19/08 03:25 PM (15 years, 7 months ago)

ah....maybe this is what I've been doing wrong. The alcohol in the shot glass idea seems like a novel approach. Anyone have an issue with that idea?


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Offlineunderdwg
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Registered: 06/13/08
Posts: 54
Loc: Gold Coast
Last seen: 13 years, 24 days
Re: DON'T alcohol after heating up syringe needle? [Re: praga]
    #8542338 - 06/19/08 09:01 PM (15 years, 7 months ago)

I had recently returned to the hobby after a short break. I have had lots of successes in the past and at one point my inoculation procedure was "on point". I could knock up 15-20 jars and never contam due to my innoc procedures. Somewhere along the way (when I switched to bags) I also picked up a nasty habit of wiping my needles after flaming. I think this was fine when I used 99% alcohol and a very clean cloth. However near the end of my attempts at bags last time I was getting a large amount of contams, bags that wouldn't finish ..etc. 

Just recently I used wronguy's bag tek (recipe) that many people have used and it has been very success full for them. However all 4 of my large spawn bags contam'd with trich (ouch). I mean not just a little either but A BAG FULL OF WHITE TRICH. At first I thought it was bad syringes but then I realized I had accidently used 70% rubbing alcohol and the rag I used to wipe was a rag I had used in the past to wipe off my big screen but forgot that was what it was used for.

Recently I knocked up 4 more bags and at RR's advice I did not wipe. So far(it has only been 6-7 days) everything looks good!! Starting to see myc growth. Things look promising!! I would highly recommend not having anything touch the syringe after flame sterilization unless you can guarantee the 100% sterility of it  (I like the alcohol in a shot glass idea).

Just flaming - allowing to cool for a slight second - and inoculating has proved the highest success rate for me in the past and proven again by my last innoc. (so far)


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Invisiblewickedsick
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Registered: 05/08/08
Posts: 701
Re: DON'T alcohol after heating up syringe needle? [Re: Sef]
    #8542345 - 06/19/08 09:02 PM (15 years, 7 months ago)

i use alchohol to cool my syringe and all my jars are producing :tongue::thumbup:


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[quote]RogerRabbit said:
You don't need to drill a lot of holes.  Some growers choose to fan several times per day instead.
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Offlineunderdwg
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Registered: 06/13/08
Posts: 54
Loc: Gold Coast
Last seen: 13 years, 24 days
Re: DON'T alcohol after heating up syringe needle? [Re: praga]
    #8542350 - 06/19/08 09:04 PM (15 years, 7 months ago)

one issue to keep in mind is that alcohol is FLAMMABLE .. doubt a flame-sterilized needle would be hot enough to ignite it by the time it reaches the shot glass but you never know. Caution advised  :psychsplit:


Edited by underdwg (06/19/08 09:05 PM)


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OfflineKagenical
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Registered: 05/27/08
Posts: 128
Last seen: 10 years, 2 months
Re: DON'T alcohol after heating up syringe needle? [Re: underdwg]
    #8542373 - 06/19/08 09:10 PM (15 years, 7 months ago)

Between innoculations and after flame sterilization, I just put the syringe cover over my needle... Y'know, the pink protective plastic covers that accompany luer lok syringes... Thing is, It's full of "vim thick bleach" :wink:.


Works wonders, I've never had a contaminate from this procedure.


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