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Sef
Warrior of Purgatory



Registered: 05/30/08
Posts: 137
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DON'T alcohol after heating up syringe needle?
#8540052 - 06/19/08 08:21 AM (15 years, 7 months ago) |
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While innoculating this last time, I decided to alcohol wipe the needle after heating it up "red hot" so the needle would cool before putting it in the jar. I just tend to hear thousands of spores screaming in pain if I dont cool the needle down somehow.
Well... I now have 36 triched out inoculation points (3 per jar). Just one mans experience on wiping with alcohol.
So! Any other ideas for cooling the needle down before poking, or is that just going to be a bad idea in general since it obviously defeats the purpose of flame sterilization to begin with?
-------------------- ~~~ Your question was probably answered below ~~~ Rye Spawning 50/50+ Casing and Casing Monotub OR Monotub When I say *I* in a post, it is simply for ease of posting. I am always, of course, referring to a friend of mine from highschool whom I haven't seen in 15 years.
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medicine man
Stranger

Registered: 02/28/08
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Re: DON'T alcohol after heating up syringe needle? [Re: Sef]
#8540071 - 06/19/08 08:28 AM (15 years, 7 months ago) |
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why do you think it was the needle? Couldn't it have been you substrate or your syringe/spores and not the needle?
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Sef
Warrior of Purgatory



Registered: 05/30/08
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Re: DON'T alcohol after heating up syringe needle? [Re: medicine man]
#8540080 - 06/19/08 08:32 AM (15 years, 7 months ago) |
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Quote:
medicine man said: why do you think it was the needle? Couldn't it have been you substrate or your syringe/spores and not the needle?
if it was the substrate, more than just the innoc points would have gotten it (it's ONLKY directly at the innoc points)
Suppose it could have been the syringe contents, but it was a batch of 3 B+ from ralphsters and the other 2 were fine.
-------------------- ~~~ Your question was probably answered below ~~~ Rye Spawning 50/50+ Casing and Casing Monotub OR Monotub When I say *I* in a post, it is simply for ease of posting. I am always, of course, referring to a friend of mine from highschool whom I haven't seen in 15 years.
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psg1
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Re: DON'T alcohol after heating up syringe needle? [Re: Sef]
#8540090 - 06/19/08 08:36 AM (15 years, 7 months ago) |
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Well, after wiping the needle with an alcohol wipe, your needle is as sterile as..... an alcohol wipe, which I would assume to be fairly contaminant free. I doubt it was your alcohol wiping that made the difference. Sounds like your syringe contents were FUBAR'd somehow. Whether it was on your end or not is anyones guess.
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RogerRabbit
Bans for Pleasure



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Re: DON'T alcohol after heating up syringe needle? [Re: Sef]
#8540101 - 06/19/08 08:40 AM (15 years, 7 months ago) |
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A red hot needle is totally sterile. An alcohol soaked towel is not sterile. Whether that's the problem or not, nobody knows, but I've recommended for years against 'cooling off' the needle. The first drop of spore solution does that job. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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xeallos
Sore wa himitsudesu!




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Re: DON'T alcohol after heating up syringe needle? [Re: RogerRabbit]
#8540163 - 06/19/08 09:00 AM (15 years, 7 months ago) |
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I've done inoculations after flaming the tip with and without alcohol wipes (even directly out of the packaging, shipped with the needle) and never noticed any substantial difference in contamination rates. I'd look elsewhere in your procedure for a more likely contamination vector.
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Mad River
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Registered: 04/29/08
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Re: DON'T alcohol after heating up syringe needle? [Re: Sef]
#8540270 - 06/19/08 09:55 AM (15 years, 7 months ago) |
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I wipe with alcohol when I do mine. I don't know if it's necessary, and may stop, but I have had zero contams.
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Neobean
Adept Mycologist


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Re: DON'T alcohol after heating up syringe needle? [Re: Mad River]
#8540286 - 06/19/08 10:03 AM (15 years, 7 months ago) |
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It's not necessary
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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legallyhomeless
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Re: DON'T alcohol after heating up syringe needle? [Re: Mad River]
#8540298 - 06/19/08 10:11 AM (15 years, 7 months ago) |
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Quote:
Mad River said: I wipe with alcohol when I do mine. I don't know if it's necessary, and may stop, but I have had zero contams.
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Digital Reality
Strangers in the dark


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Re: DON'T alcohol after heating up syringe needle? [Re: legallyhomeless]
#8540326 - 06/19/08 10:26 AM (15 years, 7 months ago) |
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What i do and have for a long time with no problems is just fill a shot glass with alcohol, flame the needle with a propane torch then dip the needle in the alcohol. I can see how there can be bacteria or whatever already on a paper towel that the alcohol might not kill which would get transferred to the needle. but i really don’t see much actually living in a bottle of alcohol. So a shot glass full has always done the trick for me. that way the needle comes in contact with nothing but alcohol.
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Mad River
Reverend, Churchof Todd



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Re: DON'T alcohol after heating up syringe needle? [Re: Digital Reality]
#8540380 - 06/19/08 10:51 AM (15 years, 7 months ago) |
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Does alcohol have any effect on spores at all? I'm not suggesting this, but if you put spores in alcohol would it kill them?
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JMR
Deviator



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Re: DON'T alcohol after heating up syringe needle? [Re: Sef]
#8540391 - 06/19/08 10:56 AM (15 years, 7 months ago) |
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How did you prepare your substrate did you pc it
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legallyhomeless
mooch


Registered: 07/01/05
Posts: 4,051
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Re: DON'T alcohol after heating up syringe needle? [Re: Mad River]
#8540396 - 06/19/08 10:58 AM (15 years, 7 months ago) |
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Quote:
Mad River said: Does alcohol have any effect on spores at all? I'm not suggesting this, but if you put spores in alcohol would it kill them?
Would you pour alcohol into an open wound on your brain?
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psg1
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Re: DON'T alcohol after heating up syringe needle? [Re: legallyhomeless]
#8540414 - 06/19/08 11:02 AM (15 years, 7 months ago) |
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Quote:
legallyhomeless said: Would you pour alcohol into an open wound on your brain?
Are we talking good 16yr scotch or.........
I guess it would depend on how bad one's day has been up to that point.
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Neobean
Adept Mycologist


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Re: DON'T alcohol after heating up syringe needle? [Re: legallyhomeless]
#8540417 - 06/19/08 11:04 AM (15 years, 7 months ago) |
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Quote:
legallyhomeless said:
Quote:
Mad River said: Does alcohol have any effect on spores at all? I'm not suggesting this, but if you put spores in alcohol would it kill them?
Would you pour alcohol into an open wound on your brain?
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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legallyhomeless
mooch


Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
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Re: DON'T alcohol after heating up syringe needle? [Re: psg1]
#8540421 - 06/19/08 11:05 AM (15 years, 7 months ago) |
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Ide say bad if your skull was cracked open exposing your brain. .....yes, 16yr scotch.
The point is no, you wouldnt, so why treat your spores like that.
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anarchOi
Ellenalien is fat.



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Re: DON'T alcohol after heating up syringe needle? [Re: legallyhomeless]
#8540518 - 06/19/08 11:41 AM (15 years, 7 months ago) |
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homless you kind of remind me of the kid who said "DON'T STOMP ON ANTS! HOW WOULD YOU LIKE TO GET STOMPED ON IF YOU WERE AN ANT?!"
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RogerRabbit
Bans for Pleasure



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Re: DON'T alcohol after heating up syringe needle? [Re: anarchOi]
#8540528 - 06/19/08 11:45 AM (15 years, 7 months ago) |
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Of course, we wouldn't put our open brain wound over a flame either, but my point was that after flame sterilizing, it isn't necessary to 'cool' the needle. I suspect his original problem was what was inside the syringe, not what was on the needle. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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nugjug
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Re: DON'T alcohol after heating up syringe needle? [Re: RogerRabbit]
#8540827 - 06/19/08 01:22 PM (15 years, 7 months ago) |
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I used to use project foam soaked in lysol as sort of a syringe condom when I did my grain inoculations. I recently did a batch of PF cakes for the first time in over a year to get quickly restarted in the hobby. I watched your DVD as a refresher course and flame sterilized and injected through micropore tape like you recommended. It is easier than trying to use a lysol covered needle when doing PF cakes as the inoculation points triple. When I was flame sterilizing the water still in the syringe kind of started boiling and pulled some substrate that had been lodged in the needle back into the syringe body. Is this because there was air in they syringe?
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Sef
Warrior of Purgatory



Registered: 05/30/08
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Re: DON'T alcohol after heating up syringe needle? [Re: nugjug]
#8540853 - 06/19/08 01:35 PM (15 years, 7 months ago) |
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Going to assume it was in the needle. Just can't see 36 individual perfect trich inoculations. I don't think I could have done a better job of inoculating trich into jars if I had been purposefully trying to grow it :P
(Yes, everything was PC fine, etc... same method I've been using) I did some rye berry noc's today in the same environment (after having been cleaned), so I'll see how those turn out.
-------------------- ~~~ Your question was probably answered below ~~~ Rye Spawning 50/50+ Casing and Casing Monotub OR Monotub When I say *I* in a post, it is simply for ease of posting. I am always, of course, referring to a friend of mine from highschool whom I haven't seen in 15 years.
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