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lipa


Registered: 07/24/07
Posts: 2,684
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Good pics of panaeolus mycelium
#8533695 - 06/17/08 01:32 PM (15 years, 10 months ago) |
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I am having a hard time finding GOOD pics of panaeolus mycelium, like with no glare. I don't care what kind, cyanescens whatever. Just would like it only 1/3-2/3 colonized across the plate. A cloned culture pic would be nice.
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MycoAu
5thKingdomCome


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Re: Good pics of panaeolus mycelium [Re: lipa]
#8535258 - 06/17/08 09:15 PM (15 years, 10 months ago) |
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P. foenisecii?
I've just transferred plate of it and can post pics in a few days. Somehow I figure it's not what you're after....
Unfortunately, I don't have any pans.
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wisp

Registered: 04/13/08
Posts: 5,304
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Re: Good pics of panaeolus mycelium [Re: MycoAu]
#8535377 - 06/17/08 09:53 PM (15 years, 10 months ago) |
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RogerRabbit
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Re: Good pics of panaeolus mycelium [Re: wisp]
#8535451 - 06/17/08 10:18 PM (15 years, 10 months ago) |
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Here's a scanning electron micrograph of a gill section of coplandia. You can see the four spores forming on each basidium, and a plurocystidia. I know it isn't a petri dish picture, but those are all on another hard drive.

Pan cyan(copelandia) mycelium can resemble mold in its early stages. Later, it's velvety white, but not rhizomorphic. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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legallyhomeless
mooch


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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
#8535513 - 06/17/08 10:36 PM (15 years, 10 months ago) |
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Great pic RR!
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Juke Adro
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Registered: 04/05/08
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I post some pics tomorrow arvo on pan myc on agar but im too busy atm
-------------------- Someone said: im actually not using ms, im using prints.
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: lipa]
#8536525 - 06/18/08 09:04 AM (15 years, 10 months ago) |
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Thank you all for your efforts. You are all great.
This is my comparison. It is from the panaeolus I found posted in the hunting forum from So Cal. Thought to be pan. tropicalis
I am on the second transfer and am isolating more colonies tonight, probably should have last night though. Anyways. These are of the samples I sent to workman for microscopy.
Germination occurred on MYA in 23 hours on the second run. First run was multi spore streak from loop. To many colonies so decided to go with dilution. Dilution was around 1/100. (I know! It looks like there was to many colonies for that dilution, It was hard to not pick up so many spores from my print). I did not gauge the first run but I think it was faster, due to heavy spore load. Growth occurs at a rate of about 2 - 2.5 mm per 12 hours and increased from a rate of 1 -1.5 mm for the first 1-2 days from inoculation. Contaminants were not too much of an issue and were fairly low for a wild print. Prints were also made on a place to place transfer on construction paper every 6 hours. Prints were dense and plentiful.



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RogerRabbit
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Re: Good pics of panaeolus mycelium [Re: lipa]
#8536830 - 06/18/08 11:17 AM (15 years, 10 months ago) |
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The top dish shows some sectoring mycelium, but the bottom dish is bacteria colonies. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
#8536877 - 06/18/08 11:30 AM (15 years, 10 months ago) |
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Quote:
RogerRabbit said: The top dish shows some sectoring mycelium, but the bottom dish is bacteria colonies. RR
Yes and no . Those might look like bacterial colonies ,but they are germinating spores "I promise". They previously looked like that when I made my first transfers. Yeah I though they were the first time around, but they are not. I wish I had a scope to show you up close. Maybe in the future. I know, the pictures make them look kinda sticky looking. It's because I let the water on the plate move over them.
Edited by lipa (06/18/08 11:37 AM)
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MycoAu
5thKingdomCome


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Re: Good pics of panaeolus mycelium [Re: lipa]
#8537080 - 06/18/08 12:50 PM (15 years, 10 months ago) |
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Using antibiotic MYA? or plain?
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: MycoAu]
#8537091 - 06/18/08 12:56 PM (15 years, 10 months ago) |
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Just plain. I don't bother with the stuff. Too $'y. You can't use the stuff when germinating spores anyways.
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MycoAu
5thKingdomCome


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Re: Good pics of panaeolus mycelium [Re: lipa]
#8538178 - 06/18/08 06:56 PM (15 years, 10 months ago) |
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I've yet to ever use it. Frankly, I've had good enough success without it by just using a few extra plates/samples. I'm surprised that you find it too expensive though. Which antibiotic do you find to be so expensive?
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RogerRabbit
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Re: Good pics of panaeolus mycelium [Re: lipa]
#8538257 - 06/18/08 07:28 PM (15 years, 10 months ago) |
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Quote:
lipa said: Just plain. I don't bother with the stuff. Too $'y. You can't use the stuff when germinating spores anyways.
I germinate spores on antibiotic agar all the time. It's preferred in fact. If those are mycelium colonies, the picture must be out of focus. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Juke Adro
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Registered: 04/05/08
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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
#8539546 - 06/19/08 02:55 AM (15 years, 10 months ago) |
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-------------------- Someone said: im actually not using ms, im using prints.
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Juke Adro
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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
#8539559 - 06/19/08 03:01 AM (15 years, 10 months ago) |
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and here is a pan myc riddled with contams which is ok cause im using them for experiments
-------------------- Someone said: im actually not using ms, im using prints.
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
#8539612 - 06/19/08 03:49 AM (15 years, 10 months ago) |
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Here's an old pic of some pan cyan mycelium just starting on PDA.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
#8540092 - 06/19/08 08:37 AM (15 years, 10 months ago) |
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Wow! how interesting. I never new that. I always thought it would hamper germination. Nice to know you can. I most often use charcoal agar for germination, this time I had a good 60 dishes already made up so I figured I better use them. I have never had the urge to buy some antibiotic solution. I have managed without it. thanks for the tip though.Of the many years that I have been doing this stuff. Granted the first 5 years were with cubes in po-dunk Oklahoma. I have never ever bought one bit of that stuff, because I never found a need for it and assumed it wouldn't work with germinating spores. Knowing that now, I can maybe use it knowing the price is worth it. I have a few prints that are dirty as hell and are such a pain in the ass, I find myself not even wanting to bother.
Yeah! not trying to say you don't know your bacteria and all. I am sure you do. I am pretty familiar with them as well. I have more plates that exhibit the same characteristics most indefinitely. I have subcultured from them and had no problems with it. It is definitely new to me thats for sure. They seem to germinate into a fuzzy ball like mass that gets areal and then starts to move across the plate. When they get wet it looks like your classic bacterial colonies until the culture grows out more.
Edited by lipa (06/19/08 09:02 AM)
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: BlimeyGrimey]
#8540095 - 06/19/08 08:38 AM (15 years, 10 months ago) |
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Thanks blimey! Thats what I am looking for. They get all puffy like that.
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
#8540102 - 06/19/08 08:40 AM (15 years, 10 months ago) |
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Man juke those are some rich plates. What is your recipe.
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
#8543624 - 06/20/08 08:34 AM (15 years, 10 months ago) |
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I tried to get better pics this morning. I chose a different plate to use for transfer because of there being to many colonies . Hope these are better for ya all. Look like pan mycelium?
Again i am thankful for your guy's input. And I got some antibiotic solution to try for the first time out of it. Thanks MycoAu!

Edited by lipa (06/20/08 08:43 AM)
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BlimeyGrimey
Collector of Spores




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Re: Good pics of panaeolus mycelium [Re: lipa]
#8543712 - 06/20/08 09:20 AM (15 years, 10 months ago) |
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Is that a spot of bacterial contam on the top left of the agar pics?
I found a pic of my last plate I posted alittle farther into colonization of the plate.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: BlimeyGrimey]
#8543763 - 06/20/08 09:33 AM (15 years, 10 months ago) |
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Yes that is bacteria. Nasty shit! Thanks for the photo.
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
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Re: Good pics of panaeolus mycelium [Re: lipa]
#8544114 - 06/20/08 11:46 AM (15 years, 10 months ago) |
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Your plates are looking good.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: BlimeyGrimey]
#8544148 - 06/20/08 11:57 AM (15 years, 10 months ago) |
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They are suspected to be Pan. tropicalis. You can see pics in my gallery under panaeolus. Workman is going to be working on assuring me of what they are.
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MycoAu
5thKingdomCome


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Re: Good pics of panaeolus mycelium [Re: lipa]
#8544293 - 06/20/08 12:41 PM (15 years, 10 months ago) |
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There is a spot in the top left of your picture (away from the majority of the growth) that appears to be bacterial.
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Juke Adro
I love peach fluff


Registered: 04/05/08
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Re: Good pics of panaeolus mycelium [Re: lipa]
#8547722 - 06/21/08 02:40 PM (15 years, 10 months ago) |
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Quote:
lipa said: Man juke those are some rich plates. What is your recipe.
chocolate malt agar
-------------------- Someone said: im actually not using ms, im using prints.
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lipa


Registered: 07/24/07
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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
#8547769 - 06/21/08 03:00 PM (15 years, 10 months ago) |
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Your kidding right! lol Really!
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Juke Adro
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Registered: 04/05/08
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Re: Good pics of panaeolus mycelium [Re: lipa]
#8547866 - 06/21/08 03:34 PM (15 years, 10 months ago) |
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nope not kidding, if it did not have the slimy texture I would eat it, its that good lol
-------------------- Someone said: im actually not using ms, im using prints.
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lipa


Registered: 07/24/07
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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
#8549020 - 06/21/08 10:19 PM (15 years, 10 months ago) |
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Such a funny guy! I'll have to try it. Thanks!
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CosmicString
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Re: Good pics of panaeolus mycelium [Re: Juke Adro]
#8549320 - 06/22/08 12:38 AM (15 years, 10 months ago) |
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Juke Adro,
The blue shroom in your sig is stunning, did you take the photo? Any information about it?
Thanks
CS
And the caterpillar is a little disturbing in that it brings back memories of an old Outer Limits show where the bugs had human like faces.
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happyday
Stranger

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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
#8549785 - 06/22/08 04:11 AM (15 years, 10 months ago) |
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lol Nice pic RR. If you stand back from a distance you can see the rim of the petri dish.
RR, We used gentamycin sulf. as antibiotic for years and thought it inhibited germination for P. mex A, while not for cubes and many others. We attempted this with same prints and syringes (we used both) on each run, and a high quality pc controlled incubator. We later switched to first germinating P. mex A spores on standard MEA, PYA, etc. and then cleaning it up later on genta s. plates. I know it's prefered to clean it up initially, but this was our conclusion for P mex A.
I will try this again to see if it was just a fluke and the spores chosen those days just happened to be of lower quality (i.e. dehydrated, aged).
Not sure but maybe it was Stamets who said running your mycelium continously on antibiotic could later cause your cultures to be less resistant against bad bacteria and less able to utilize their own defense mechanisms. That's perhaps the case with all organisms when given excessive amounts of antiotics.
btw Lipa, those two plates in these recent pics look like mycelium to me. At the beginning(first photos) the bottom two did look like possible bacteria, except the two sectoring plates above them. Now you can clearly see 3-D. However it might or might not be a polyculture. If they are result of spores from outdoors, then I find it hard to believe it's not a mix.
I would run with a clean cut from the plate on your first photos, attempt to fruit on a few different substrates, and then see what happens. Oh, what did the spores resemble under the microscope?
Edited by happyday (06/22/08 04:24 AM)
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lipa


Registered: 07/24/07
Posts: 2,684
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Re: Good pics of panaeolus mycelium [Re: happyday]
#8550131 - 06/22/08 08:57 AM (15 years, 10 months ago) |
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I am definite that some of the plates are harboring contaminants. I have transfered a lot of colonies into new plates and isolating for now. They are looking good though. I don't get my new microscope until Tuesday. I am soooo excited! Workman pointed me to a good little setup and the college is going to help me calibrate it so I can take measurements. Ye ha!
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MycoAu
5thKingdomCome


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Re: Good pics of panaeolus mycelium [Re: lipa]
#8551269 - 06/22/08 04:50 PM (15 years, 10 months ago) |
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Juke's pic of the caterpillar is from the movie "Labyrinth". Great movie. Villianous vile creature it is.
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Juke Adro
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Registered: 04/05/08
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Re: Good pics of panaeolus mycelium [Re: CosmicString]
#8552614 - 06/22/08 11:35 PM (15 years, 10 months ago) |
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Quote:
CosmicString said: Juke Adro,
The blue shroom in your sig is stunning, did you take the photo? Any information about it?
Thanks
CS
And the caterpillar is a little disturbing in that it brings back memories of an old Outer Limits show where the bugs had human like faces.
no i did not take the pic wish i did, the mushroom is a Entoloma hochstetteri
-------------------- Someone said: im actually not using ms, im using prints.
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panmanPE91
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Re: Good pics of panaeolus mycelium [Re: RogerRabbit]
#18074582 - 04/07/13 10:29 PM (11 years, 1 month ago) |
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in yours or anyones experience how did colonizing panaeolus substrates smell? particularly pf tek with manure in mason jars.
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Juke Adro
I love peach fluff


Registered: 04/05/08
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Re: Good pics of panaeolus mycelium [Re: panmanPE91]
#18074665 - 04/07/13 10:43 PM (11 years, 1 month ago) |
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Mushroom, Earthy.
In future mate if you can't find your answer by search, just make a new thread In mush cult This is advanced section and the thread is old as hell. You will get a lot more answers making a new thread
-------------------- Someone said: im actually not using ms, im using prints.
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